AB124971

Anti-RAD52 antibody [EPR3464(2)]

RabMAb
Recombinant
What is this?

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(11 Publications)

Rabbit Recombinant Monoclonal RAD52 antibody. Suitable for WB and reacts with Human samples. Cited in 11 publications.

View Alternative Names

DNA repair protein RAD52 homolog, RAD52

3 Images

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Western blot - Anti-RAD52 antibody [EPR3464(2)] (AB124971)

All lanes:

Western blot - Anti-RAD52 antibody [EPR3464(2)] (ab124971) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

PC3 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 46 kDa

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Lab

Western blot - Anti-RAD52 antibody [EPR3464(2)] (AB124971)

All lanes:

Western blot - Anti-RAD52 antibody [EPR3464(2)] (ab124971) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 46 kDa

Observed band size: 48 kDa

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OI-RD Scanning - Anti-RAD52 antibody [EPR3464(2)] (AB124971)

OI-RD Scanning

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OI-RD Scanning - Anti-RAD52 antibody [EPR3464(2)] (AB124971)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3464(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAD52 also known by alternate names such as DNA repair protein RAD52 homolog functions as an important player in DNA repair mechanisms. It aids in the homologous recombination and repair of DNA double-strand breaks. RAD52 has a molecular mass of approximately 49 kDa and is mainly expressed in the nucleus. Its ability to bind to DNA and facilitate strand annealing are important for its mechanical function. RAD52 expression varies across tissues but it is prominently observed in rapidly dividing cells and tissues with high rates of DNA repair activity.

Biological function summary

DNA repair protein RAD52 homolog engages significantly in maintaining genome stability. RAD52 forms complexes with other DNA repair proteins such as RAD51 and RPA to perform its role efficiently. The protein supports the loading of RAD51 onto single-stranded DNA a critical step in homologous recombination. By stabilizing RAD51 nucleoprotein filaments RAD52 promotes efficient repair and recombination processes necessary for cellular survival after DNA damage.

Pathways

The activities of RAD52 link to the homologous recombination and DNA damage response pathways. RAD52 operates alongside proteins like RAD51 and BRCA2 to mediate the repair of DNA double-strand breaks through homologous recombination. This relationship is important for maintaining genomic integrity especially during the S and G2 phases of the cell cycle. The synergy between RAD52 and other double-strand break repair proteins ensures effective resolution of DNA damage.

The malfunction or dysregulation of RAD52 connects to conditions like cancer and Fanconi anemia. Deficiencies or mutations in RAD52 can compromise DNA repair and lead to genomic instability a hallmark of cancer. Additionally in Fanconi anemia the interplay between RAD52 and the FA complex is impaired influencing the DNA repair capacity. The disrupted interaction with other repair proteins including those in the BRCA family further suggests RAD52's important role in disease pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.

See full target information RAD52

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Nature 629:697-703 PubMed38658755

2024

Mechanism of single-stranded DNA annealing by RAD52-RPA complex.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Chao Liang,Luke A Greenhough,Laura Masino,Sarah Maslen,Ilirjana Bajrami,Marcel Tuppi,Mark Skehel,Ian A Taylor,Stephen C West

iScience 27:108925 PubMed38323009

2024

RNA helicase DDX3 regulates RAD51 localization and DNA damage repair in Ewing sarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew E Randolph,Marwa Afifi,Aparna Gorthi,Rachel Weil,Breelyn A Wilky,Joshua Weinreb,Paul Ciero,Natalie Ter Hoeve,Paul J van Diest,Venu Raman,Alexander J R Bishop,David M Loeb

Molecular cell 82:3985-4000.e4 PubMed36265486

2022

TERRA and RAD51AP1 promote alternative lengthening of telomeres through an R- to D-loop switch.

Applications

Unspecified application

Species

Unspecified reactive species

Tribhuwan Yadav,Jia-Min Zhang,Jian Ouyang,Wendy Leung,Antoine Simoneau,Lee Zou

Nature communications 12:512 PubMed33479235

2021

Oncogenic herpesvirus KSHV triggers hallmarks of alternative lengthening of telomeres.

Applications

Unspecified application

Species

Unspecified reactive species

Timothy P Lippert,Paulina Marzec,Aurora I Idilli,Grzegorz Sarek,Aleksandra Vancevska,Mark Bower,Paul J Farrell,Päivi M Ojala,Niklas Feldhahn,Simon J Boulton

Molecular cell 81:1027-1042.e4 PubMed33453166

2021

Alternative lengthening of telomeres is a self-perpetuating process in ALT-associated PML bodies.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Min Zhang,Marie-Michelle Genois,Jian Ouyang,Li Lan,Lee Zou

Nucleic acids research 48:12778-12791 PubMed33275133

2020

The function of RAD52 N-terminal domain is essential for viability of BRCA-deficient cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kritika Hanamshet,Alexander V Mazin

Cell division 15:12 PubMed33292330

2020

Chromosomal aberration arises during somatic reprogramming to pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Liu,Conghui Li,Kang Zheng,Xiaofeng Zhao,Xiaofeng Xu,Aifen Yang,Min Yi,Huaping Tao,Binghua Xie,Mengsheng Qiu,Junlin Yang

Cell 175:558-570.e11 PubMed30245011

2018

Human Rad52 Promotes XPG-Mediated R-loop Processing to Initiate Transcription-Associated Homologous Recombination Repair.

Applications

Unspecified application

Species

Unspecified reactive species

Takaaki Yasuhara,Reona Kato,Yoshihiko Hagiwara,Bunsyo Shiotani,Motohiro Yamauchi,Shinichiro Nakada,Atsushi Shibata,Kiyoshi Miyagawa

Scientific reports 8:11525 PubMed30069034

2018

RDM1 plays an oncogenic role in human lung adenocarcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Tong,Jian Liu,Wangjun Yan,Wenjiao Cao,Shihui Shen,Kun Li,Lei Li,Guoping Niu

Cell death and differentiation 25:1063-1079 PubMed29511339

2018

p73 coordinates with Δ133p53 to promote DNA double-strand break repair.

Applications

Unspecified application

Species

Unspecified reactive species

Hongjian Gong,Yuxi Zhang,Kunpeng Jiang,Shengfan Ye,Shuming Chen,Qinghe Zhang,Jinrong Peng,Jun Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RAD52 antibody [EPR3464(2)]

Western blot - Anti-RAD52 antibody [EPR3464(2)] (AB124971)

Western blot - Anti-RAD52 antibody [EPR3464(2)] (AB124971)

OI-RD Scanning - Anti-RAD52 antibody [EPR3464(2)] (AB124971)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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