Anti-Rad53 antibody

• WB

AbReview33397****

Western blot - Anti-Rad53 antibody (AB104232)

Blocking : 4% milk for 1 hour

Phosphorylation of Rad53 induced by phleomycin treatment see PubmedID : 152856

All lanes:

Western blot - Anti-Rad53 antibody (ab104232) at 1/2000 dilution

Lane 1:

Saccharomyces cerevisiae whole cell lysate from exponentially growing cells- TCA prep. at 20 µg

Lane 2:

Saccharomyces cerevisiae whole cell lysate from exponentially growing cells treated with 20mg/ml phleomycin for 1 hour - TCA prep at 20 µg

Lane 3:

Saccharomyces cerevisiae whole cell lysate from exponentially growing cells- TCA prep at 20 µg

Lane 4:

Saccharomyces cerevisiae whole cell lysate from exponentially growing cells arrested in G2 phase with nocodazole- TCA prep. at 20 µg

Lane 5:

Saccharomyces cerevisiae whole cell lysate from exponentially growing cells arrested in G2 phase with nocodazole and treated with 20mg/ml phleomycin- TCA prep. at 20 µg

Secondary

All lanes:

Amersham anti-rabbit IgG conjugated to HRP at 1/10000 dilution

Predicted band size: 92 kDa

Observed band size: 92 kDa,>92 kDa

true

Exposure time: 2min

This image is courtesy of an abreview submitted by Michela Clerici, University of Milano-Biococca

• WB

Collaborator

Western blot - Anti-Rad53 antibody (AB104232)

All lanes:

Western blot - Anti-Rad53 antibody (ab104232) at 1/2000 dilution

Lane 1:

TCA preps from exponentially growing WT yeast cells.

Lane 2:

TCA preps from exponentially growing WT yeast cells treated with Hydroxyurea (HU).

Lane 3:

TCA preps from exponentially growing rad53delta yeast cells.

Secondary

All lanes:

1/10000 dilution

Predicted band size: 92 kDa

true

Exposure time: 30s

This image is courtesy of Ilaria Guerini, Gurdon Institute, United Kingdom

• WB

AbReview30978****

Western blot - Anti-Rad53 antibody (AB104232)

All lanes:

Western blot - Anti-Rad53 antibody (ab104232) at 1/1000 dilution

Lanes 1 and 5:

Alpha-factor arrested WT cells.

Lane 2:

WT cells 20' after release in S-phase.

Lane 3:

WT cells 40' after release in S-phase.

Lane 4:

WT cells 60' after release in S-phase.

Lane 6:

WT cells 20' after release in S-phase in 200mM HU.

Lane 7:

WT cells 40' after release in S-phase in 200mM HU.

Lane 8:

WT cells 60' after release in S-phase in 200mM HU.

Lanes 9 and 13:

Alpha-factor arrested rad53delta cells.

Lane 10:

rad53delta cells 20' after release in S-phase.

Lane 11:

rad53delta cells 40' after release in S-phase.

Lane 12:

rad53delta cells 60' after release in S-phase.

Lane 14:

rad53delta cells 20' after release in S-phase in 200mM HU.

Lane 15:

rad53delta cells 40' after release in S-phase in 200mM HU.

Lane 16:

rad53delta cells 60' after release in S-phase in 200mM HU.

Secondary

All lanes:

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/donkey-rabbit-igg-h-l-hrp-ab16284'>ab16284</a>) at 1/2000 dilution

Predicted band size: 92 kDa

Observed band size: 92 kDa

true

Exposure time: 2min

• WB

AbReview32873****

Western blot - Anti-Rad53 antibody (AB104232)

Western blot showing ab104232 detecting Rad53 in both its unphosphorylated and phosphorylated status. ab104232 was used at 1 : 2000 dilution.

All lanes:

Western blot - Anti-Rad53 antibody (ab104232)

Predicted band size: 92 kDa

true

Exposure time: 1min