AB166859

Anti-Rad53 antibody [EL7.E1]

Name: Anti-Rad53 antibody [EL7.E1] (ab166859) | Abcam
Brand: Abcam Limited
SKU: AB166859
Price: 405 USD
Availability: InStock

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(30 Publications)

Mouse Monoclonal RAD53 antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 30 publications.

View Alternative Names

MEC2, SAD1, SPK1, YPL153C, P2588, RAD53, Serine/threonine-protein kinase RAD53, CHEK2 homolog, Serine-protein kinase 1

3 Images

Lab

Western blot - Anti-Rad53 antibody [EL7.E1] (AB166859)

Western Blot showing ab166859 detecting both unphosphorylated and phosphorylated Rad53.

Blocking buffer : 3% Milk

Developed using the ECL technique.

Performed under reducing conditions.

Additional bands at : 55 kDa. Identity unknown.

All lanes:

Western blot - Anti-Rad53 antibody [EL7.E1] (ab166859) at 1 µg/mL

Lane 1:

S. Cerevisiae untreated at 5 µL

Lane 2:

S. Cerevisiae MMS treated at 5 µL

Secondary

All lanes:

HRP conjugated Goat Anti-Mouse IgG (H+L) at 1/5000 dilution

Predicted band size: 92 kDa

Observed band size: 100 kDa

false

Exposure time: 4min

Lab

Western blot - Anti-Rad53 antibody [EL7.E1] (AB166859)

Western Blot showing ab166859 detecting both unphosphorylated and phosphorylated Rad53.

All lanes:

Western blot - Anti-Rad53 antibody [EL7.E1] (ab166859) at 1 µg/mL

Lane 1:

TCA preps from S. cerevisiae cells at 15 µg

Lane 2:

TCA preps from S. cerevisiae cells treated with Hydroxyurea (HU) at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/10000 dilution

Predicted band size: 92 kDa

Observed band size: 50 kDa,92 kDa

true

Exposure time: 20min

CiteAb

Western blot - Anti-Rad53 antibody [EL7.E1] (AB166859)

Rad53 western blot using anti-Rad53 antibody [EL7.E1] ab166859. Publication image and figure legend from Vanoli, F., Fumasoni, M., et al., 2010, PLoS Genet, PubMed 21085632.

ab166859 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab166859 please see the product overview.

RPA, promoting the strand invasion step of homologous recombination, is required for template switch replication.(A) sgs1 (HY1461) and sgs1 rfa1-t11 (HY1459) were synchronized in G2 with nocodazole and released in medium containing MMS 0.033% at 28°C. The replication intermediates were digested with NcoI and analyzed with the ARS305 probe. (B) Exponentially growing wild-type (W303-1A) and rfa1-t11 (HY1464) cells were treated for 2 and 4 hours with MMS 0.02%. Western blot analysis was performed to detect Rad53 phosphorylation.

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

EL7.E1

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Saccharomyces cerevisiae

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rad53 also known as Chk2 is a serine/threonine-protein kinase with a molecular mass of approximately 89 kDa. It plays an essential role in the DNA damage response. It is primarily expressed in the nucleus of eukaryotic cells. Rad53 phosphorylates substrates involved in the cell cycle and DNA repair processes. This protein acts as a signal transducer in the DNA damage checkpoint pathway helping to maintain genomic stability.

Biological function summary

Rad53 regulates the cell cycle by mediating the response to DNA damage. It is part of the conserved cell cycle checkpoint signaling network. This protein interacts with several other checkpoint proteins including Mec1 and Tel1. It modulates the replication fork stabilization and recovery during DNA replication stress. The ability of Rad53 to detect and respond to DNA damage events is key for proper cell cycle progression and the prevention of chromosomal aberrations.

Pathways

Rad53 remains a central component of the DNA damage checkpoint pathway and the replication checkpoint pathway. Rad53 works closely with Mec1 which is an upstream kinase that activates Rad53 in response to DNA damage. The pathway involves other proteins like Dun1 and Sml1 which Rad53 regulates to initiate a proper cellular response. By engaging in these pathways Rad53 contributes to DNA repair and replication fork stability.

Rad53 plays a critical role in cancer and genetic instability disorders. Dysfunctional Rad53 activity can lead to unchecked cell proliferation contributing to the development of cancer. It interacts with proteins such as p53 which is often mutated in cancers. Mutations affecting Rad53 or its associated signaling pathway components can lead to reduced DNA repair capacity increasing susceptibility to genomic instability disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Controls S-phase checkpoint as well as G1 and G2 DNA damage checkpoints. Phosphorylates proteins on serine, threonine, and tyrosine. Prevents entry into anaphase and mitotic exit after DNA damage via regulation of the Polo kinase CDC5. Seems to be involved in the phosphorylation of RPH1.

See full target information RAD53

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Publications (30)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:984 PubMed40604292

2025

Continuous nuclear envelope surveillance is required for DNA double strand break repair.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Medina-Suárez,Félix Machín

eLife 13: PubMed39656839

2024

Prolonged cell cycle arrest in response to DNA damage in yeast requires the maintenance of DNA damage signaling and the spindle assembly checkpoint.

Applications

Unspecified application

Species

Unspecified reactive species

Felix Y Zhou,David P Waterman,Marissa Ashton,Suhaily Caban-Penix,Gonen Memisoglu,Vinay V Eapen,James E Haber

Nature communications 15:10532 PubMed39627228

2024

Stabilization of expandable DNA repeats by the replication factor Mcm10 promotes cell viability.

Applications

Unspecified application

Species

Unspecified reactive species

Chiara Masnovo,Zohar Paleiov,Daniel Dovrat,Laurel K Baxter,Sofia Movafaghi,Amir Aharoni,Sergei M Mirkin

iScience 27:110250 PubMed39021806

2024

Msc1 is a nuclear envelope protein that reinforces DNA repair in late mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Medina-Suárez,Jessel Ayra-Plasencia,Lara Pérez-Martínez,Falk Butter,Félix Machín

microPublication biology 2024: PubMed38287928

2024

The CRISPR/Cas9 system forms a condensate in the yeast nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Medina-Suárez,Félix Machín

Nature communications 14:1227 PubMed36869098

2023

Genetic requirements for repair of lesions caused by single genomic ribonucleotides in S phase.

Applications

Unspecified application

Species

Unspecified reactive species

Natalie Schindler,Matthias Tonn,Vanessa Kellner,Jia Jun Fung,Arianna Lockhart,Olga Vydzhak,Thomas Juretschke,Stefanie Möckel,Petra Beli,Anton Khmelinskii,Brian Luke

Nature communications 13:2374 PubMed35501303

2022

Mre11-Rad50 oligomerization promotes DNA double-strand break repair.

Applications

Unspecified application

Species

Unspecified reactive species

Vera M Kissling,Giordano Reginato,Eliana Bianco,Kristina Kasaciunaite,Janny Tilma,Gea Cereghetti,Natalie Schindler,Sung Sik Lee,Raphaël Guérois,Brian Luke,Ralf Seidel,Petr Cejka,Matthias Peter

Genetics 219: PubMed34849883

2021

Rad9-mediated checkpoint activation is responsible for elevated expansions of GAA repeats in CST-deficient yeast.

Applications

Unspecified application

Species

Unspecified reactive species

Ekaterina Spivakovsky-Gonzalez,Erica J Polleys,Chiara Masnovo,Jorge Cebrian,Adrian M Molina-Vargas,Catherine H Freudenreich,Sergei M Mirkin

DNA repair 95:102939 PubMed32777450

2020

Checkpoint adaptation in recombination-deficient cells drives aneuploidy and resistance to genotoxic agents.

Applications

Unspecified application

Species

Unspecified reactive species

Olga Vydzhak,Katharina Bender,Julia Klermund,Anke Busch,Stefanie Reimann,Brian Luke

Cell reports 30:2094-2105.e9 PubMed32075754

2020

Sen1 Is Recruited to Replication Forks via Ctf4 and Mrc1 and Promotes Genome Stability.

Applications

Unspecified application

Species

Unspecified reactive species

Rowin Appanah,Emma Claire Lones,Umberto Aiello,Domenico Libri,Giacomo De Piccoli

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Rad53 antibody [EL7.E1]

Western blot - Anti-Rad53 antibody [EL7.E1] (AB166859)

Western blot - Anti-Rad53 antibody [EL7.E1] (AB166859)

Western blot - Anti-Rad53 antibody [EL7.E1] (AB166859)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (30)

Product promise