AB11055

Anti-RAD54 antibody [Rad54 4E3/1]

Name: Anti-RAD54 antibody [Rad54 4E3/1] (ab11055) | Abcam
Brand: Abcam Limited
SKU: AB11055
Price: 500 USD
Availability: InStock
Rating: 4 (4 reviews)

(4 Reviews)

(7 Publications)

Mouse Monoclonal RAD54 antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Full Length Protein corresponding to Human RAD54L.

View Alternative Names

RAD54A, RAD54L, DNA repair and recombination protein RAD54-like, RAD54 homolog, hHR54, hRAD54

1 Images

Flow Cytometry - Anti-RAD54 antibody [Rad54 4E3/1] (AB11055)

Flow Cyt

Unknown

Flow Cytometry - Anti-RAD54 antibody [Rad54 4E3/1] (AB11055)

Overlay histogram showing HeLA cells stained with ab11055 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11055, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Rad54 4E3/1

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IP, WB, ICC/IF, Flow Cyt

applications

Immunogen

Full Length Protein corresponding to Human RAD54L. The exact immunogen used to generate this antibody is proprietary information.

Q92698

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAD54 also known as DNA repair and recombination protein RAD54-like (Swiss-Prot Q9Y620) plays a mechanical role in DNA repair processes. It is part of the RAD52 epistasis group and shows DNA-dependent ATPase activity. RAD54 belongs to the DEAD-like helicase superfamily. It is approximately 82 kDa in mass and gets expressed in normal human tissues with higher levels in the testis. RAD54 has an ATPase activity modulating chromatin architecture making the DNA more accessible for repair enzymes.

Biological function summary

RAD54 contributes to homologous recombination an important mechanism for repairing double-strand breaks in DNA. It forms a complex with RAD51 facilitating RAD51-mediated homologous pairing and strand exchange. Through this interaction RAD54 promotes efficient DNA synapsis stabilizing the formation of joint molecules and protecting the DNA from further damage. RAD54 also unwinds DNA and removes RAD51 from dsDNA thereby resolving recombination intermediates.

Pathways

RAD54 functions in the homologous recombination repair pathway. This pathway involves several key proteins including BRCA1 and RAD51 which collaborate to repair double-strand DNA breaks and maintain genome stability. RAD54's work with these molecules ensures the accurate repair of damaged DNA. RAD54 also links to the Fanconi anemia pathway contributing to DNA damage response and crosslink repair.

RAD54 associates with increased cancer susceptibility due to its role in DNA repair. Mutations or dysregulation of RAD54 can lead to genomic instability resulting in cancer types like breast cancer. RAD54 links to BRCA1 in this context highlighting its importance in preserving DNA integrity. Studies also show connections between RAD54 and developmental disorders resulting from impaired DNA repair mechanisms that affect cell growth and division.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays an essential role in homologous recombination (HR) which is a major pathway for repairing DNA double-strand breaks (DSBs), single-stranded DNA (ssDNA) gaps, and stalled or collapsed replication forks (PubMed : 11459989, PubMed : 12205100, PubMed : 24798879, PubMed : 27264870, PubMed : 32457312, PubMed : 9774452). Acts as a molecular motor during the homology search and guides RAD51 ssDNA along a donor dsDNA thereby changing the homology search from the diffusion-based mechanism to a motor-guided mechanism. Also plays an essential role in RAD51-mediated synaptic complex formation which consists of three strands encased in a protein filament formed once homology is recognized. Once DNA strand exchange occured, dissociates RAD51 from nucleoprotein filaments formed on dsDNA (By similarity).

See full target information RAD54L

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell biology international 49:1274-1289 PubMed40616532

2025

RAD54L Is a Prognostic Biomarker and Demonstrate Correlation With Drug Sensitivity in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting You,Hui Tang,Hui Ge,Chunmei Bai,Jianfeng Zhou

Nature communications 11:2639 PubMed32457312

2020

The bromodomain containing protein BRD-9 orchestrates RAD51-RAD54 complex formation and regulates homologous recombination-mediated repair.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Zhou,Jinzhou Huang,Chao Zhang,Fei Zhao,Wootae Kim,Xinyi Tu,Yong Zhang,Somaira Nowsheen,Qian Zhu,Min Deng,Yuping Chen,Bo Qin,Kuntian Luo,Baohua Liu,Zhenkun Lou,Robert W Mutter,Jian Yuan

Cancer discovery 7:984-998 PubMed28588062

2017

Secondary Somatic Mutations Restoring and Associated with Acquired Resistance to the PARP Inhibitor Rucaparib in High-Grade Ovarian Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Olga Kondrashova,Minh Nguyen,Kristy Shield-Artin,Anna V Tinker,Nelson N H Teng,Maria I Harrell,Michael J Kuiper,Gwo-Yaw Ho,Holly Barker,Maria Jasin,Rohit Prakash,Elizabeth M Kass,Meghan R Sullivan,Gregory J Brunette,Kara A Bernstein,Robert L Coleman,Anne Floquet,Michael Friedlander,Ganessan Kichenadasse,David M O'Malley,Amit Oza,James Sun,Liliane Robillard,Lara Maloney,David Bowtell,Heidi Giordano,Matthew J Wakefield,Scott H Kaufmann,Andrew D Simmons,Thomas C Harding,Mitch Raponi,Iain A McNeish,Elizabeth M Swisher,Kevin K Lin,Clare L Scott

Cancer research 74:3546-55 PubMed24753542

2014

The RAD51-stimulatory compound RS-1 can exploit the RAD51 overexpression that exists in cancer cells and tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer M Mason,Hillary L Logan,Brian Budke,Megan Wu,Michal Pawlowski,Ralph R Weichselbaum,Alan P Kozikowski,Douglas K Bishop,Philip P Connell

Nucleic acids research 42:5616-32 PubMed24598253

2014

PARP3 affects the relative contribution of homologous recombination and nonhomologous end-joining pathways.

Applications

ICC/IF

Species

Human

Carole Beck,Christian Boehler,Josée Guirouilh Barbat,Marie-Elise Bonnet,Giuditta Illuzzi,Philippe Ronde,Laurent R Gauthier,Najat Magroun,Anbazhagan Rajendran,Bernard S Lopez,Ralph Scully,François D Boussin,Valérie Schreiber,Françoise Dantzer

The Journal of biological chemistry 285:13761-8 PubMed20215648

2010

Contributions of nucleotide excision repair, DNA polymerase eta, and homologous recombination to replication of UV-irradiated herpes simplex virus type 1.

Applications

Species

Human

Isabella Muylaert,Per Elias

Molecular biology of the cell 19:2926-35 PubMed18463164

2008

Rad54B targeting to DNA double-strand break repair sites requires complex formation with S100A11.

Applications

Unspecified application

Species

Human

Ulrike Murzik,Peter Hemmerich,Stefanie Weidtkamp-Peters,Tobias Ulbricht,Wendy Bussen,Julia Hentschel,Ferdinand von Eggeling,Christian Melle

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RAD54 antibody [Rad54 4E3/1]

Flow Cytometry - Anti-RAD54 antibody [Rad54 4E3/1] (AB11055)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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