Rabbit Polyclonal Rad9 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human RAD9A.
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- Cellular signalling 86:1100912021FOXP1 and NDRG1 act differentially as downstream effectors of RAD9-mediated prostate cancer cell functions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSunil K Panigrahi,Constantinos G Broustas,Ping Q Cuiper,Renu K Virk,Howard B LiebermanPubMed 34298089
- Nature communications 11:1232020Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClémence Mooser et. al.PubMed 31913317
- Molecular cell 74:571-583.e82019MDC1 Interacts with TOPBP1 to Maintain Chromosomal Stability during Mitosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPia-Amata Leimbacher et. al.PubMed 30898438
- Scientific reports 9:3512019Three Prime Repair Exonuclease 1 (TREX1) expression correlates with cervical cancer cells growth in vitro and disease progression in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBruna Prati et. al.PubMed 30674977
- Carcinogenesis 40:164-1722018RAD9A promotes metastatic phenotypes through transcriptional regulation of anterior gradient 2 (AGR2).Applications:Unspecified applicationReactive species:Unspecified reactive speciesConstantinos G Broustas,Kevin M Hopkins,Sunil K Panigrahi,Li Wang,Renu K Virk,Howard B LiebermanPubMed 30295739
- Genes & development 31:46-582017PHF11 promotes DSB resection, ATR signaling, and HR.
- Nucleic acids research 43:4531-462015Regulation of NEIL1 protein abundance by RAD9 is important for efficient base excision repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSunil K Panigrahi et. al.PubMed 25873625
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human RAD9A. The exact immunogen used to generate this antibody is proprietary information. Database link Q99638
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Bat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Ferret | Predicted | Predicted | Predicted |
| Gorilla | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Pig, Ferret, Rhesus monkey, Gorilla, Bat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Pig, Ferret, Rhesus monkey, Gorilla, Bat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Pig, Ferret, Rhesus monkey, Gorilla, Bat | Dilution info - | Notes - |
Target data
Function
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair (PubMed:10713044, PubMed:17575048, PubMed:20545769, PubMed:21659603, PubMed:31135337). The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex (PubMed:21659603). Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER) (PubMed:21659603). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates (PubMed:21659603). The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase (PubMed:21659603). RAD9A possesses 3'->5' double stranded DNA exonuclease activity (PubMed:10713044).
Alternative names
Cell cycle checkpoint control protein RAD9A, hRAD9, DNA repair exonuclease rad9 homolog A, RAD9A
Recommended products
Rabbit Polyclonal Rad9 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human RAD9A.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human RAD9A. The exact immunogen used to generate this antibody is proprietary information. Database link Q99638
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Rad9 also known as RAD9A is an important component in the cell cycle checkpoint control and DNA repair mechanisms. The Rad9 protein has a molecular weight of approximately 48 kDa. It functions as a part of the 9-1-1 complex a sensor of DNA damage during cell cycle progression. Rad9 is expressed mainly in the nucleus of cells where it participates in recognizing and binding to DNA at sites of damage. Through its interaction with other checkpoint proteins it facilitates the recruitment and activation of enzymes involved in DNA repair.
- Biological function summary
The Rad9 protein plays a central role in maintaining genomic stability. It acts as a DNA damage sensor and is part of the heterotrimeric 9-1-1 complex consisting of Rad9 Rad1 and Hus1. This complex helps signal the presence of DNA damage to halt the cell cycle allowing time for repair mechanisms to resolve issues. Rad9 helps prevent mutations that can lead to cell death or cancer development by ensuring effective DNA repair processes.
- Pathways
The Rad9 protein functions in the DNA damage response and cell cycle checkpoint pathways. As part of these pathways it interacts with proteins such as ATR and Chk1 which are responsible for transmitting signals that initiate cell cycle arrest and DNA repair. The 9-1-1 complex with Rad9 as a critical component links the detection of DNA lesions to the activation of downstream effector proteins facilitating an appropriate cellular response to DNA damage.
- Associated diseases and disorders
Rad9 has been linked to cancer and other conditions involving genomic instability. Alterations in Rad9 function can impair the DNA damage response leading to the accumulation of mutations that predispose cells to cancerous transformations. Furthermore the protein's relationship with BRCA1 a well-known tumor suppressor gene highlights its role in breast cancer susceptibility. Disruptions in the Rad9 associated pathways may also contribute to other disorders where DNA repair is compromised such as certain types of anemias and other syndromes characterized by genomic instability.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-Rad9 antibody (ab70810)
All lanes: Western blot - Anti-Rad9 antibody (ab70810) at 0.1 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Lane 4: 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 43 kDa
Observed band size: 55 kDa
Exposure time: 3min
Immunoprecipitation - Anti-Rad9 antibody (ab70810)
1mg whole cell lysate from HeLa cells was immunoprecipitated using ab70810 at 3ug/mg of lysate (lane 1) or a control rabbit Ig (lane 2). For the subsequent western blot, 20% of the immunoprecipitate was loaded per lane, and probed with ab70810 at 1ug/ml.
Detection: chemiluminescence with exposure time of 30 seconds.All lanes: Immunoprecipitation - Anti-Rad9 antibody (ab70810)
Predicted band size: 43 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad9 antibody (ab70810)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Rad9 with ab70810 at 1/1000 (1µg/ml). Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad9 antibody (ab70810)
IHC image of ab70810 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab70810, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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