AB251318

Anti-RAET1E antibody [EPR17443] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal RAET1E antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

LETAL, N2DL4, ULBP4, UNQ1867/PRO4303, RAET1E, Retinoic acid early transcript 1E, Lymphocyte effector toxicity activation ligand, NKG2D ligand 4, RAE-1-like transcript 4, UL16-binding protein 4, N2DL-4, NKG2DL4

4 Images

Supplier Data

Immunoprecipitation - Anti-RAET1E antibody [EPR17443] - BSA and Azide free (AB251318)

This data was developed using ab200662, the same antibody clone in a different buffer formulation.

RAET1E was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab200662 at 1/100 dilution.

Lane 1 : HeLa whole cell lysate 10 μg (Input).
Lane 2 : ab200662 IP in HeLa whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab200662 in HeLa whole cell lysate.

Western blot was performed using ab200662 at 1/2000 dilution.

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-RAET1E antibody [EPR17443] (<a href='/en-us/products/primary-antibodies/raet1e-antibody-epr17443-ab200662'>ab200662</a>)

Predicted band size: 30 kDa

false

Supplier Data

Western blot - Anti-RAET1E antibody [EPR17443] - BSA and Azide free (AB251318)

This data was developed using ab200662, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RAET1E antibody [EPR17443] (<a href='/en-us/products/primary-antibodies/raet1e-antibody-epr17443-ab200662'>ab200662</a>) at 1/5000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma) cell lysate at 20 µg

Lane 2:

HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µg

Lane 3:

MCF-7 (Human breast adenocarcinoma cell line) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 30 kDa

Observed band size: 30 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-RAET1E antibody [EPR17443] - BSA and Azide free (AB251318)

This data was developed using ab200662, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RAET1E antibody [EPR17443] (<a href='/en-us/products/primary-antibodies/raet1e-antibody-epr17443-ab200662'>ab200662</a>) at 1/1000 dilution

All lanes:

Human fetal brain tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 30 kDa

Observed band size: 30 kDa

false

Exposure time: 1min

Supplier Data

Western blot - Anti-RAET1E antibody [EPR17443] - BSA and Azide free (AB251318)

This data was developed using ab200662, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RAET1E antibody [EPR17443] (<a href='/en-us/products/primary-antibodies/raet1e-antibody-epr17443-ab200662'>ab200662</a>) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cells) cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) cell lysate at 10 µg

Lane 4:

NIH/3T3 (Mouse embyro fibroblast cells) cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 30 kDa

Observed band size: 30 kDa

false

Exposure time: 10s

Unconjugated

Anti-RAET1E antibody [EPR17443]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17443

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab251318 is the carrier-free version of ab200662.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAET1E also known as UL16-binding protein 4 (ULBP4) is a member of the MHC class I-related molecules. It has a molecular mass of approximately 31 kDa. RAET1E is part of the family of proteins that acts as a ligand for the NKG2D receptor found on the surface of natural killer (NK) cells. In terms of expression RAET1E is present on the surface of various cell types especially in stressed transformed or virus-infected cells where it signals cellular distress to immune cells.

Biological function summary

RAET1E plays a role in immune surveillance. It is involved in the activation of NK cells when it binds to the NKG2D receptor. This interaction is part of the body's defense mechanism against transformed or infected cells. RAET1E operates in isolation rather than as part of a larger protein complex which allows it to independently initiate the immune response when detecting harmful cells.

Pathways

RAET1E functions within the innate immune response pathway. It works closely with the NKG2D signaling pathway which is important for the activation of cytotoxic activities in immune cells. RAET1E through its binding to NKG2D facilitates the destruction of malignant cells. This connection places RAET1E alongside other similar NKG2D ligands such as MIC and other ULBP family proteins which contribute to the activation of NK and T cells.

RAET1E is linked to various cancer types and infectious diseases. Its expression on the surface of tumor cells can signal NK cells to target and destroy these potentially harmful cells. In certain cancers the loss or reduction of RAET1E expression may allow tumor cells to escape immune detection. In viral infections the upregulation of RAET1E on infected cells assists in the clearance of viruses by NK cells. Both in cancer and viral infections proteins like RAET1E and NKG2D play an important part in the immune system's defensive strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds and activates the KLRK1/NKG2D receptor, mediating natural killer cell cytotoxicity.

See full target information RAET1E

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular cancer research : MCR 21:1107-1119 PubMed37409971

2023

TRIM50 Inhibits Gastric Cancer Progression by Regulating the Ubiquitination and Nuclear Translocation of JUP.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajia Hu,Runjie Huang,Chengcai Liang,Yingnan Wang,Min Wang,Yanxing Chen,Chenyi Wu,Jinling Zhang,Zekun Liu,Qi Zhao,Zexian Liu,Feng Wang,Shuqiang Yuan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RAET1E antibody [EPR17443] - BSA and Azide free

Immunoprecipitation - Anti-RAET1E antibody [EPR17443] - BSA and Azide free (AB251318)

Western blot - Anti-RAET1E antibody [EPR17443] - BSA and Azide free (AB251318)

Western blot - Anti-RAET1E antibody [EPR17443] - BSA and Azide free (AB251318)

Western blot - Anti-RAET1E antibody [EPR17443] - BSA and Azide free (AB251318)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Proteins & Peptides

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise