AB137435

Anti-Raf1 antibody

Name: Anti-Raf1 antibody (ab137435) | Abcam
Brand: Abcam Limited
SKU: AB137435
Price: 400 USD
Availability: InStock
Rating: 5 (2 reviews)

Lab Essentials
What is this?

(2 Reviews)

(38 Publications)

Rabbit Polyclonal RAF1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 38 publications.

View Alternative Names

RAF, RAF1, RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, Raf-1, cRaf

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-Raf1 antibody (AB137435)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Raf1 antibody (AB137435)

Immunofluorescence analysis of paraformaldehyde-fixed A431 cells labelling Raf1 with ab137435 at 1/200 dilution. The image in the lower panel is merged with a DNA probe.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raf1 antibody (AB137435)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raf1 antibody (AB137435)

Immunohistochemical analysis of parafin-embedded A549 xenograft labelling Raf1 with ab137435 at 1/500 dilution.

ICC/IF

AbReview43171****

Immunocytochemistry/ Immunofluorescence - Anti-Raf1 antibody (AB137435)

Immunocytochemistry/Immunofluorescence analysis A549 cells labeling Raf1 with ab137435 at 1/200 dilution. The cells were fixed with Paraformaldehyde and permeabilized with 0.1% v/v Triton X-100 pH 7.4. CF568 Donkey anti-rabbit IgG(H+L) polyclonal was used as the secondary antibody.

This image is courtesy of an AbReview submitted by Dimitra Kalamida.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raf1 antibody (AB137435)

Immunohistochemical analysis of formalin-fixed paraffin-embedded human endometrial carcinoma tissue section labeling Raf1 with ab137435 at 1/500.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raf1 antibody (AB137435)

Immunohistochemical analysis of paraffin-embedded mouse muscle tissue labeling Raf1 with ab137435 at 1/500 dilution. Antigen Retrieval : EDTA based buffer, pH 8.0, 15min. Nuclear staining is observed.

Supplier Data

Western blot - Anti-Raf1 antibody (AB137435)

Raf1 protein may migrate to higher size when phosphorylated and appears as doublet due to different phosphorylation status.

7.5% SDS gel.

Running condition : 80V, 15min; 140V, 40 min.

Transfer condition : Semi-dry, 18 V, 60 min (NC membrane).

Blocking condition : 5% non-fat milk in TBST, RT, 60 min.

Primary antibody incubation : 4°C overnight.

Washing condition : 5 ml TBST, 4 x 5 min.

Exposure : Trident plus Western HRP Substrate.

All lanes:

Western blot - Anti-Raf1 antibody (ab137435) at 1/1000 dilution

Lane 1:

Untreated (–) HeLa whole cell extracts starved for 24 hours at 30 µg

Lane 2:

200nM PMA treated (+) HeLa whole cell extracts starved for 24 hours at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 73 kDa

Observed band size: 150 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Raf1 also known as c-Raf or Raf-1 is a serine/threonine-protein kinase that plays an important role in cell division differentiation and survival. Its molecular weight is approximately 74 kDa. Raf1 is part of the MAPK/ERK signaling pathway and is ubiquitously expressed in many tissues including the brain heart and liver. As a component of the signaling cascade its activation translates extracellular signals into intracellular responses.

Biological function summary

Raf1 regulates important cellular processes by activating downstream kinases in response to external stimuli. Raf1 forms a complex with other proteins such as Ras facilitating its role as an essential component of the MAPK/ERK pathway. Upon activation by Ras Raf1 phosphorylates and activates MEK1 and MEK2 which in turn activate the ERK1 and ERK2. This signaling axis is involved in controlling gene expression and cellular proliferation.

Pathways

Raf1 is integrally involved in the MAPK/ERK pathway which is critical for transducing signals from growth factors and mitogens. It relates closely with proteins such as Ras MEK and ERK in this pathway. The pathway is important for regulating cellular responses to various stimuli and is particularly involved in processes such as cell cycle control and apoptosis.

Raf1 has associations with certain types of cancer and Noonan syndrome. In various cancers mutations or dysregulation of Raf1 or related components such as Ras can lead to uncontrolled cellular proliferation. In Noonan syndrome Raf1 mutations result in anomalies in the Ras/MAPK pathway which can impact normal development. Raf1’s interactions in these pathways indicate its relevance as a potential target for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases : ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

See full target information RAF1

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Publications (38)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 17:105264 PubMed40697228

2025

Nav1.6 drives colorectal cancer proliferation and invasion through MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Ming Zhao,Wan-Ying Hong,Jian-Guang Xu,Shui-Quan Lin,Ming-Sheng Liu,Li-Hui Wang,Xu-Li Jiang,Ming Sang,Yang-Bo Lv

CNS neuroscience & therapeutics 31:e70172 PubMed39753851

2025

Integrated Mendelian Randomization and Single-Cell Transcriptomics Analysis Identifies Critical Blood Biomarkers and Potential Mechanisms in Epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Jianwei Shi,Jing Xie,Yanfeng Yang,Bin Fu,Zuliang Ye,Ting Tang,Quanlei Liu,Jinkun Xu,Penghu Wei,Yongzhi Shan,Guoguang Zhao

BMC biology 22:176 PubMed39183304

2024

Casein kinase 1α mediates estradiol secretion via CYP19A1 expression in mouse ovarian granulosa cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Luo,Di Zhang,Jiaming Zheng,Hui Liu,Longjie Sun,Hongzhou Guo,Lei Wang,Sheng Cui

Cancer innovation 3:e117 PubMed38947754

2024

MAPK4 facilitates angiogenesis by inhibiting the ERK pathway in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Chen,Jing Yang,Yufang Liu,Xu Zhao,Juanjuan Zhao,Lin Tang,Mengmeng Guo,Ya Zhou,Chao Chen,Dongmei Li,Zhenke Wen,Guiyou Liang,Lin Xu

Theranostics 14:3583-3602 PubMed38948067

2024

Extracellular vesicles from apoptotic BMSCs ameliorate osteoporosis via transporting regenerative signals.

Applications

Unspecified application

Species

Unspecified reactive species

Maojiao Li,Qi Tang,Chengcheng Liao,Zhuo Wang,Siyuan Zhang,Qingqing Liang,Cheng Liang,Xiaodong Liu,Jingyi Zhang,Weidong Tian,Li Liao

Molecular nutrition & food research 67:e2200577 PubMed36907948

2023

Enhanced Hypoglycemic Bioactivity via RAS/Raf-1/MEK/ERK Signaling Pathway by Combining Capsaicin and QUERCETIN from Chili Peppers.

Applications

Unspecified application

Species

Unspecified reactive species

Si Mi,Wenxuan Zhu,Xiangnan Zhang,Yuhang Wang,Tong Li,Xianghong Wang

Cell death discovery 8:271 PubMed35614051

2022

GPR110 promotes progression and metastasis of triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hye-Jung Nam,Yeon-Ju Kim,Jae-Hyeok Kang,Su-Jae Lee

Oncology letters 24:223 PubMed35720479

2022

BPTF activates the MAPK pathway through coexpression with Raf1 to promote proliferation of T-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dongyu Bai,Yong Zhou,Fayan Shen,Dehong Gao,Wenhao Suo,Haiping Zhang,Heng Li

Annals of translational medicine 10:314 PubMed35433952

2022

RAF1 mediates the FSH signaling pathway as a downstream molecule to stimulate estradiol synthesis and secretion in mouse ovarian granulosa cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Luo,Hui Liu,Hongzhou Guo,Longjie Sun,Kemian Gou,Sheng Cui

BMC cancer 22:298 PubMed35313850

2022

Correlation of LAGE3 with unfavorable prognosis and promoting tumor development in HCC via PI3K/AKT/mTOR and Ras/RAF/MAPK pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Li,Hui Xiong

View all publications

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