Rabbit Polyclonal RAF1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 29 publications.
Images
Publications
Filter by
- Molecular nutrition & food research 67:e22005772023Enhanced Hypoglycemic Bioactivity via RAS/Raf-1/MEK/ERK Signaling Pathway by Combining Capsaicin and QUERCETIN from Chili Peppers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSi Mi,Wenxuan Zhu,Xiangnan Zhang,Yuhang Wang,Tong Li,Xianghong WangPubMed 36907948
- Cell death discovery 8:2712022GPR110 promotes progression and metastasis of triple-negative breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHye-Jung Nam,Yeon-Ju Kim,Jae-Hyeok Kang,Su-Jae LeePubMed 35614051
- Oncology letters 24:2232022BPTF activates the MAPK pathway through coexpression with Raf1 to promote proliferation of T-cell lymphoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongyu Bai,Yong Zhou,Fayan Shen,Dehong Gao,Wenhao Suo,Haiping Zhang,Heng LiPubMed 35720479
- Annals of translational medicine 10:3142022RAF1 mediates the FSH signaling pathway as a downstream molecule to stimulate estradiol synthesis and secretion in mouse ovarian granulosa cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuan Luo,Hui Liu,Hongzhou Guo,Longjie Sun,Kemian Gou,Sheng CuiPubMed 35433952
- BMC cancer 22:2982022Correlation of LAGE3 with unfavorable prognosis and promoting tumor development in HCC via PI3K/AKT/mTOR and Ras/RAF/MAPK pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun Li,Hui XiongPubMed 35313850
- Genetics and molecular biology 44:e202004632021Overexpression of RAPGEF3 enhances the therapeutic effect of dezocine in treatment of neuropathic pain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXue Liu,Li Song,Xiaojun Ma,Yong Liu,Hui Huang,Yongsheng Xu,Wei YanPubMed 34807222
- Experimental & molecular medicine 53:1356-13652021MicroRNA-338-3p as a novel therapeutic target for intervertebral disc degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHua Jiang,Abu Moro,Jiaqi Wang,Dihua Meng,Xinli Zhan,Qingjun WeiPubMed 34531509
- Molecular medicine reports 23:2021Diagnostic value of microRNA‑125b in peripheral blood mononuclear cells for pulmonary tuberculosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaolu Sun et. al.PubMed 33537800
- Scientific reports 10:111582020Blocking C-Raf alleviated high-dose small-volume radiation-induced epithelial mesenchymal transition in mice lung.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhen-Yu Hong et. al.PubMed 32636458
- International journal of molecular medicine 46:965-9762020Downregulation of microRNA‑143 promotes osteogenic differentiation of human adipose‑derived mesenchymal stem cells through the k‑Ras/MEK/ERK signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYiming Zhang et. al.PubMed 32582994
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Predicted | Predicted | Predicted |
| Chicken | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Cow, Xenopus tropicalis | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Cow, Xenopus tropicalis | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Cow, Xenopus tropicalis | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.
Alternative names
RAF, RAF1, RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, Raf-1, cRaf
Recommended products
Rabbit Polyclonal RAF1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 29 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Raf1 also known as c-Raf or Raf-1 is a serine/threonine-protein kinase that plays an important role in cell division differentiation and survival. Its molecular weight is approximately 74 kDa. Raf1 is part of the MAPK/ERK signaling pathway and is ubiquitously expressed in many tissues including the brain heart and liver. As a component of the signaling cascade its activation translates extracellular signals into intracellular responses.
- Biological function summary
Raf1 regulates important cellular processes by activating downstream kinases in response to external stimuli. Raf1 forms a complex with other proteins such as Ras facilitating its role as an essential component of the MAPK/ERK pathway. Upon activation by Ras Raf1 phosphorylates and activates MEK1 and MEK2 which in turn activate the ERK1 and ERK2. This signaling axis is involved in controlling gene expression and cellular proliferation.
- Pathways
Raf1 is integrally involved in the MAPK/ERK pathway which is critical for transducing signals from growth factors and mitogens. It relates closely with proteins such as Ras MEK and ERK in this pathway. The pathway is important for regulating cellular responses to various stimuli and is particularly involved in processes such as cell cycle control and apoptosis.
- Associated diseases and disorders
Raf1 has associations with certain types of cancer and Noonan syndrome. In various cancers mutations or dysregulation of Raf1 or related components such as Ras can lead to uncontrolled cellular proliferation. In Noonan syndrome Raf1 mutations result in anomalies in the Ras/MAPK pathway which can impact normal development. Raf1’s interactions in these pathways indicate its relevance as a potential target for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Western blot - Anti-Raf1 antibody (ab137435)
Raf1 protein may migrate to higher size when phosphorylated and appears as doublet due to different phosphorylation status.
7.5% SDS gel.
Running condition: 80V, 15min; 140V, 40 min.
Transfer condition: Semi-dry, 18 V, 60 min (NC membrane).
Blocking condition: 5% non-fat milk in TBST, RT, 60 min.
Primary antibody incubation: 4°C overnight.
Washing condition: 5 ml TBST, 4 x 5 min.
Exposure: Trident plus Western HRP Substrate.
All lanes: Western blot - Anti-Raf1 antibody (ab137435) at 1/1000 dilution
Lane 1: Untreated (–) HeLa whole cell extracts starved for 24 hours at 30 µg
Lane 2: 200nM PMA treated (+) HeLa whole cell extracts starved for 24 hours at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 73 kDa
Observed band size: 150 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raf1 antibody (ab137435)
Immunohistochemical analysis of paraffin-embedded mouse muscle tissue labeling Raf1 with ab137435 at 1/500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15min. Nuclear staining is observed.
This image is courtesy of a customer review submitted by Dimitra Kalamida.Immunocytochemistry/ Immunofluorescence - Anti-Raf1 antibody (ab137435)
Immunocytochemistry/Immunofluorescence analysis A549 cells labeling Raf1 with ab137435 at 1/200 dilution. The cells were fixed with Paraformaldehyde and permeabilized with 0.1% v/v Triton X-100 pH 7.4. CF568 Donkey anti-rabbit IgG(H+L) polyclonal was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raf1 antibody (ab137435)
Immunohistochemical analysis of formalin-fixed paraffin-embedded human endometrial carcinoma tissue section labeling Raf1 with ab137435 at 1/500.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raf1 antibody (ab137435)
Immunohistochemical analysis of parafin-embedded A549 xenograft labelling Raf1 with ab137435 at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Raf1 antibody (ab137435)
Immunofluorescence analysis of paraformaldehyde-fixed A431 cells labelling Raf1 with ab137435 at 1/200 dilution. The image in the lower panel is merged with a DNA probe.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com