AB326656

Anti-Raf1 (phospho S43) antibody [SR2182]

Recombinant
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Rabbit Recombinant Monoclonal RAF1 phospho S43 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

RAF, RAF1, RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, Raf-1, cRaf

3 Images

Supplier Data

Western blot - Anti-Raf1 (phospho S43) antibody [SR2182] (AB326656)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : MCF7 whole cell lysates
Lane 2 : MCF7 treated with 200nM PMA for 30min whole cell lysates
Lane 3 : MCF7 treated with Lambda Protein Phosphatase for 30min whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size : 73 kDa
Observed band size : 65-75 kDa

Exposure time : 7 seconds

All lanes:

Western blot - Anti-Raf1 (phospho S43) antibody [SR2182] (ab326656) at 1/1000 dilution

Lane 1:

MCF7 whole cell lysates at 20 µg

Lane 2:

MCF7 treated with 200nM PMA for 30min whole cell lysates at 20 µg

Lane 3:

MCF7 treated with Lambda Protein Phosphatase for 30min whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 73 kDa

Observed band size: 65-75 kDa

false

Exposure time: 7s

Supplier Data

Western blot - Anti-Raf1 (phospho S43) antibody [SR2182] (AB326656)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : MCF7 whole cell lysates
Lane 2 : Hela whole cell lysates
Lane 3 : HepG2 whole cell lysates
Lane 4 : 293T whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size : 73 kDa
Observed band size : 65-75 kDa

Exposure time : 10 seconds

All lanes:

Western blot - Anti-Raf1 (phospho S43) antibody [SR2182] (ab326656) at 1/1000 dilution

Lane 1:

MCF7 whole cell lysates at 20 µg

Lane 2:

Hela whole cell lysates at 20 µg

Lane 3:

HepG2 whole cell lysates at 20 µg

Lane 4:

293T whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 73 kDa

Observed band size: 65-75 kDa

false

Exposure time: 10s

Supplier Data

Western blot - Anti-Raf1 (phospho S43) antibody [SR2182] (AB326656)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : 3T3 whole cell lysates
Lane 2 : Mouse Heart lysates
Lane 3 : Rat Brain lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size : 73 kDa
Observed band size : 65-75 kDa

Exposure time : 10 seconds

All lanes:

Western blot - Anti-Raf1 (phospho S43) antibody [SR2182] (ab326656) at 1/1000 dilution

Lane 1:

3T3 whole cell lysates at 20 µg

Lane 2:

Mouse Heart lysates at 20 µg

Lane 3:

Rat Brain lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 73 kDa

Observed band size: 65-75 kDa

false

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SR2182

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases : ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

See full target information RAF1 pS43

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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