AB233438

Anti-Raftlin antibody

Name: Anti-Raftlin antibody (ab233438) | Abcam
Brand: Abcam Limited
SKU: AB233438

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(3 Publications)

Rabbit Polyclonal Raftlin antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RFTN1 aa 250-550.

View Alternative Names

KIAA0084, MIG2, RFTN1, Raftlin, Cell migration-inducing gene 2 protein, Raft-linking protein

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raftlin antibody (AB233438)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raftlin antibody (AB233438)

Formalin-fixed, paraffin-embedded human liver tissue stained for Raftlin using ab233438 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raftlin antibody (AB233438)

Formalin-fixed, paraffin-embedded human kidney tissue stained for Raftlin using ab233438 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raftlin antibody (AB233438)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for Raftlin using ab233438 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raftlin antibody (AB233438)

Formalin-fixed, paraffin-embedded human prostate tissue stained for Raftlin using ab233438 at 10 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-Raftlin antibody (AB233438)

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Western blot - Anti-Raftlin antibody (ab233438) at 3 µg/mL

All lanes:

Recombinant human Raftlin protein

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 63 kDa

false

Supplier Data

Western blot - Anti-Raftlin antibody (AB233438)

All lanes:

Western blot - Anti-Raftlin antibody (ab233438) at 3 µg/mL

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 63 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RFTN1 aa 250-550. The exact immunogen used to generate this antibody is proprietary information.

Q14699

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Raftlin also known as RFTN1 is an important glycoprotein in cellular mechanics. It has a molecular mass of approximately 180 kDa. Raftlin is broadly expressed in various cell types including immune and epithelial cells. It plays a role mainly in cell membrane components often found in lipid rafts contributing to signal transduction processes.

Biological function summary

Raftlin plays a significant role in immune system functioning. It is important in the formation and stability of lipid rafts specialized membrane microdomains that facilitate efficient signaling. Raftlin associates with the B-cell receptor complex stabilizing complex formation and affecting antibody-mediated immune responses. Through these interactions Raftlin influences the cellular signaling cascade essential for proper immune defense.

Pathways

Raftlin integrates into key immune signaling routes such as the B cell receptor (BCR) signaling pathway. It modulates the recruitment and assembly of signaling molecules influencing pathways like NF-kB essential for immune cell activation. Raftlin interacts with proteins like Lyn and Syk important for signal propagation within these pathways enhancing immune response regulation.

Raftlin is linked to immune-related conditions such as autoimmune diseases. It associates with proteins like CD19 and plays a part in dysregulated BCR signaling which can contribute to pathologies such as lupus. Alterations in Raftlin expression or function can impact immune tolerance and lead to improper immune system activation thereby driving disease processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in protein trafficking via association with clathrin and AP2 complex (PubMed : 21266579, PubMed : 27022195). Upon bacterial lipopolysaccharide stimulation, mediates internalization of TLR4 to endosomes in dendritic cells and macrophages; and internalization of poly(I : C) to TLR3-positive endosomes in myeloid dendritic cells and epithelial cells; resulting in activation of TICAM1-mediated signaling and subsequent IFNB1 production (PubMed : 21266579, PubMed : 27022195). Involved in T-cell antigen receptor-mediated signaling by regulating tyrosine kinase LCK localization, T-cell dependent antibody production and cytokine secretion (By similarity). May regulate B-cell antigen receptor-mediated signaling (PubMed : 12805216). May play a pivotal role in the formation and/or maintenance of lipid rafts (PubMed : 12805216).

See full target information RFTN1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

British journal of pharmacology 182:5391-5408 PubMed40660634

2025

Scorpion venom heat-resistant synthetic peptide improves cognitive dysfunction of APP/PS1 mice through microglial retromer complex.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Zhang,Yu-Xia Wu,Xiao-Gang Zhang,Song-Yu Guo,Jia He,Na-Na Hu,Yue-Lin Huang,Yue Kong,Qi-Fa Li,Ao-Ran Sui,Bao-Hang Zhu,Hua Piao,Jie Zhao,Shao Li

Biomolecules 12: PubMed36139155

2022

Expression of Major Lipid Raft Protein Raftlin in Chronic Rhinosinusitis with Nasal Polyps in Smoking and Non-Smoking Patients Correlated with Interleukin-17 and Tumor Necrosis Factor-α Levels.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Tsai Lin,Ming-Hsien Tsai,Yan-Ye Su,Wei-Chih Chen,Shun-Chen Huang,Chih-Yen Chien

BMC cancer 21:257 PubMed33750346

2021

Multi-omics analysis of genomics, epigenomics and transcriptomics for molecular subtypes and core genes for lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Zhao,Yakun Gao,Xiaodong Xu,Jiwu Zhou,He Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Raftlin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raftlin antibody (AB233438)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raftlin antibody (AB233438)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raftlin antibody (AB233438)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raftlin antibody (AB233438)

Western blot - Anti-Raftlin antibody (AB233438)

Western blot - Anti-Raftlin antibody (AB233438)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise