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AB128196

Anti-Rag A antibody

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Rabbit Polyclonal Rag A antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RRAGA.

View Alternative Names

Ras-related GTP-binding protein A, Rag A, RagA, Adenovirus E3 14.7 kDa-interacting protein 1, FIP-1, RRAGA

3 Images
Western blot - Anti-Rag A antibody (AB128196)
  • WB

Supplier Data

Western blot - Anti-Rag A antibody (AB128196)

All lanes:

Western blot - Anti-Rag A antibody (ab128196) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

MCF7 cell lysate at 20 µg

Lane 3:

MOLT4 cell lysate at 20 µg

Lane 4:

NIH-3T3 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 37 kDa

false

Western blot - Anti-Rag A antibody (AB128196)
  • WB

Unknown

Western blot - Anti-Rag A antibody (AB128196)

All lanes:

Western blot - Anti-Rag A antibody (ab128196) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 10 µg

Predicted band size: 37 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-Rag A antibody (AB128196)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Rag A antibody (AB128196)

HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.5% TritonX 100 and reacted with anti-Rag A antibody at 1/100 dilution. As the 2nd antibody, anti-rabbit IgG antibody conjugated with Alexa Fluor 647 was used at 1/1,000 dilution. DNA was stained with DAPI and the merged image was shown on right.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human RRAGA.

Q7L523

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mammals": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
pH: 6 - 8.5 Preservative: 0.05% Sodium azide Constituents: 99% Whole serum
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rag A is a small GTPase also known as Ras-related GTP-binding protein A. It plays a role in cellular growth and proliferation. The protein operates by binding to GTP and GDP functioning as a molecular switch. Rag A's molecular weight is approximately 42 kDa. It is expressed in many tissues throughout the body indicating its widespread role in cellular processes.
Biological function summary

Rag A interacts with amino acids and nutrient signaling by activating the mTORC1 complex a central regulator of cell growth. It ensures correct localization of mTORC1 to the lysosomal surface facilitating its activation. The mTORC1 complex includes other proteins such as RAPTOR and mLST8 which collectively modulate cell metabolism and protein synthesis. Rag A's participation is essential for sensing nutrients and the energy state within cells.

Pathways

With regards to pathways Rag A is involved in the mTOR signaling pathway and the autophagy-lysosome pathway. These two pathways are important to maintaining cellular homeostasis and responding to nutrient availability. Rag A directly interacts with Rag C another protein in this pathway to relay the presence of amino acids to mTORC1. Through these interactions Rag A aids in the regulation of cellular growth energy management and metabolism in response to environmental cues.

Rag A has connections to conditions like cancer and metabolic disorders. Abnormal activation of the mTOR pathway often involving Rag A can lead to uncontrolled cell proliferation seen in cancers. Additionally the protein's role in nutrient sensing links Rag A to metabolic disorders like diabetes. Alterations in Rag A activity can influence the mTORC1 pathway and misregulate associated proteins such as TSC2 contributing to disease development and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade (PubMed : 20381137, PubMed : 24095279, PubMed : 25936802, PubMed : 31601708, PubMed : 31601764, PubMed : 38103557). Forms heterodimeric Rag complexes with RagC/RRAGC or RagD/RRAGD and cycles between an inactive GDP-bound and an active GTP-bound form : RagA/RRAGA is in its active form when GTP-bound RagA/RRAGA forms a complex with GDP-bound RagC/RRAGC (or RagD/RRAGD) and in an inactive form when GDP-bound RagA/RRAGA heterodimerizes with GTP-bound RagC/RRAGC (or RagD/RRAGD) (PubMed : 20381137, PubMed : 24095279, PubMed : 25936802, PubMed : 31601708, PubMed : 31601764, PubMed : 32868926). In its GTP-bound active form, promotes the recruitment of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB (PubMed : 20381137, PubMed : 25936802, PubMed : 31601708, PubMed : 31601764). Involved in the RCC1/Ran-GTPase pathway (PubMed : 9394008). May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death (PubMed : 8995684).. (Microbial infection) May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death.
See full target information RRAGA
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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