Rabbit Polyclonal RAG2 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RAG2_HUMAN aa 250 to C-terminus.
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Publications
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- Journal of immunology (Baltimore, Md. : 1950) 206:2271-22762021Cutting Edge: TCR-β Selection Is Required at the CD4CD8 Stage of Human T Cell Development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEdward L Y Chen,Patrick M Brauer,Elisa C Martinez,Xiaotian Huang,Ning Yu,Michele K Anderson,Yang Li,Juan Carlos Zúñiga-PflückerPubMed 33941655
- The Journal of clinical investigation 124:1672-842014CDK4 deficiency promotes genomic instability and enhances Myc-driven lymphomagenesis.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RAG2_HUMAN aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P55895
Reactivity data
Select an application| IHC-P | ICC/IF | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Cow | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow, Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow, Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3 (By similarity).
Alternative names
V(D)J recombination-activating protein 2, RAG-2, RAG2
Recommended products
Rabbit Polyclonal RAG2 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RAG2_HUMAN aa 250 to C-terminus.
Alternative names
V(D)J recombination-activating protein 2, RAG-2, RAG2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RAG2_HUMAN aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P55895
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RAG2 also known as Recombination Activating Gene 2 plays an important role in the mechanism of V(D)J recombination aiding in the assembly of T-cell receptors and immunoglobulins. It acts together with RAG1 as a heterodimer complex. RAG2 lacks enzymatic activity but assists in this complex by binding to RAG1 facilitating recombinase activity. The molecular weight of RAG2 is around 56 kDa and it is mainly expressed in developing lymphocytes within the thymus and bone marrow.
- Biological function summary
RAG2 contributes to lymphocyte development by enabling the production of diverse antigen receptor repertoires. It acts as a non-catalytic partner with RAG1 forming a complex necessary for the cleavage of DNA at specific recombination signal sequences. This complexity is fundamental in generating the variability of antibodies and T-cell receptors. RAG2 alters the chromatin structure enhancing accessibility to the recombination substrates and ensuring efficient gene rearrangement.
- Pathways
RAG2 is essential in the adaptive immune response being vital to V(D)J recombination in lymphocyte development. It operates extensively in the antigen receptor gene assembly pathway influencing B cell development and T cell maturation. Throughout these processes RAG2 works closely with proteins like RAG1 where both are co-dependent for functional recombinase activity essential for immune system adaptability.
- Associated diseases and disorders
Mutations or malfunctions in RAG2 can lead to immunodeficiency syndromes such as severe combined immunodeficiency (SCID) and Omenn syndrome. These disorders often stem from defective or inefficient V(D)J recombination causing improper immune cell function. RAG2's relationship with RAG1 is critical as defects in this complex impair lymphocyte development leading to reduced immune response capability aligned with these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-RAG2 antibody (ab95955)
All lanes: Western blot - Anti-RAG2 antibody (ab95955) at 1/1000 dilution
All lanes: MOLT4 whole cell lysate at 30 µg
Predicted band size: 59 kDa
Immunocytochemistry/ Immunofluorescence - Anti-RAG2 antibody (ab95955)
ab95955, at a 1/200 dilution, staining RAG2 in paraformaldehyde fixed HeLa by Immunofluorescence analysis.
The image on the right was co-stained using Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAG2 antibody (ab95955)
ab95955, at a 1/100 dilution, staining RAG2 in paraffin embedded DLD1 Xenograft by Immunohistochemistry.
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