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AB95955

Anti-RAG2 antibody

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(2 Publications)

Rabbit Polyclonal RAG2 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RAG2_HUMAN aa 250 to C-terminus.

View Alternative Names

V(D)J recombination-activating protein 2, RAG-2, RAG2

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-RAG2 antibody (AB95955)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RAG2 antibody (AB95955)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling RAG2 protein at nucleus using ab95955 at a 1/500 dilution (Green).
Anti phalloidin antibody was used at 1/200 dilution (Red)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAG2 antibody (AB95955)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAG2 antibody (AB95955)

ab95955, at a 1/100 dilution, staining RAG2 in paraffin embedded DLD1 Xenograft by Immunohistochemistry.

Western blot - Anti-RAG2 antibody (AB95955)
  • WB

Supplier Data

Western blot - Anti-RAG2 antibody (AB95955)

Samples were separated by 0.075 SDS-PAGE.

All lanes:

Western blot - Anti-RAG2 antibody (ab95955) at 1/1000 dilution

All lanes:

Rat thymus lysate at 50 µg

Predicted band size: 59 kDa

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Western blot - Anti-RAG2 antibody (AB95955)
  • WB

Unknown

Western blot - Anti-RAG2 antibody (AB95955)

All lanes:

Western blot - Anti-RAG2 antibody (ab95955) at 1/1000 dilution

All lanes:

MOLT4 whole cell lysate at 30 µg

Predicted band size: 59 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RAG2_HUMAN aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P55895

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAG2 also known as Recombination Activating Gene 2 plays an important role in the mechanism of V(D)J recombination aiding in the assembly of T-cell receptors and immunoglobulins. It acts together with RAG1 as a heterodimer complex. RAG2 lacks enzymatic activity but assists in this complex by binding to RAG1 facilitating recombinase activity. The molecular weight of RAG2 is around 56 kDa and it is mainly expressed in developing lymphocytes within the thymus and bone marrow.
Biological function summary

RAG2 contributes to lymphocyte development by enabling the production of diverse antigen receptor repertoires. It acts as a non-catalytic partner with RAG1 forming a complex necessary for the cleavage of DNA at specific recombination signal sequences. This complexity is fundamental in generating the variability of antibodies and T-cell receptors. RAG2 alters the chromatin structure enhancing accessibility to the recombination substrates and ensuring efficient gene rearrangement.

Pathways

RAG2 is essential in the adaptive immune response being vital to V(D)J recombination in lymphocyte development. It operates extensively in the antigen receptor gene assembly pathway influencing B cell development and T cell maturation. Throughout these processes RAG2 works closely with proteins like RAG1 where both are co-dependent for functional recombinase activity essential for immune system adaptability.

Mutations or malfunctions in RAG2 can lead to immunodeficiency syndromes such as severe combined immunodeficiency (SCID) and Omenn syndrome. These disorders often stem from defective or inefficient V(D)J recombination causing improper immune cell function. RAG2's relationship with RAG1 is critical as defects in this complex impair lymphocyte development leading to reduced immune response capability aligned with these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps : a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends : 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3 (By similarity).
See full target information RAG2_HUMAN
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 206:2271-2276 PubMed33941655

2021

Cutting Edge: TCR-β Selection Is Required at the CD4CD8 Stage of Human T Cell Development.

Applications

Unspecified application

Species

Unspecified reactive species

Edward L Y Chen,Patrick M Brauer,Elisa C Martinez,Xiaotian Huang,Ning Yu,Michele K Anderson,Yang Li,Juan Carlos Zúñiga-Pflücker

The Journal of clinical investigation 124:1672-84 PubMed24614102

2014

CDK4 deficiency promotes genomic instability and enhances Myc-driven lymphomagenesis.

Applications

WB

Species

Human

Yuanzhi Lu,Yongsheng Wu,Xiaoling Feng,Rulong Shen,Jing H Wang,Mohammad Fallahi,Weimin Li,Chunying Yang,William Hankey,Weiqiang Zhao,Ramesh K Ganju,Ming O Li,John L Cleveland,Xianghong Zou
View all publications
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Product promise

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