Anti-RAGE antibody

• WB

AbReview6956****

Western blot - Anti-RAGE antibody (AB37647)

The band at 52kDa is a tubulin loading control.

All lanes:

Western blot - Anti-RAGE antibody (ab37647) at 1/500 dilution

Lane 1:

HUVEC whole cell lysate at 20 µg

Lane 2:

EVC304 whole cell lysate at 20 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit

Predicted band size: 42 kDa

Observed band size: 17 kDa,40 kDa

true

Exposure time: 4min

This image is courtesy of an anonymous Abreview

• Flow Cyt

AbReview12603****

Flow Cytometry - Anti-RAGE antibody (AB37647)

ab37647 staining human Jurkat T cells by Flow Cytometry. The cells were prepared in PBS with 0.2% BSA. The primary antibody diluted 1/100 and incubated with sample for 30 minutes at 0°C. The secondary antibody was Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG, diluted 1/200.

Specimen tube 006 is negative control

This image is courtesy of an anonymous Abreview

• Flow Cyt

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Flow Cytometry - Anti-RAGE antibody (AB37647)

RAW 264.7 cells were stained with anti-RAGE (1ug/million cells) for 1hr at 4 deg C followed by staining with anti-rabbit IgG-PE conjugate. Cells were analyzed by flow cytometry. Unstained cells or cells stained with secondary antibody alone are represented in the background

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-RAGE antibody (AB37647)

ICC/IF image of ab37647 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37647, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• WB

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Western blot - Anti-RAGE antibody (AB37647)

Bovine RAGE is slightly larger than human and mouse RAGE - 44kDa compared with 42kDa

All lanes:

Western blot - Anti-RAGE antibody (ab37647) at 1/2000 dilution

Lane 1:

Bovine lung extract

Lane 2:

Mouse lung extract

Lane 3:

HeLa cells expressing human soluble RAGE

Secondary

All lanes:

anti-rabbit IgG alkaline phosphatase conjugate

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

• ICC/IF

AbReview16881****

Immunocytochemistry/ Immunofluorescence - Anti-RAGE antibody (AB37647)

ab37647 at a 1/100 dilution staining RAGE in bovine endothelial cells by Immunocytochemistry/ Immunofluorescence. Fixed in PFA, permeabilized with Triton X-100. Blocked using 10% serum for 20 minutes at room temperature. Secondary used at 1/200 polyclonal Goat snti-rabbit conjugated to Alexa Fluor 568 (red). Nuclear staining (blue).

This image was kindly supplied by Dr Fabien Gosselet by Abreview