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AB241499

Anti-RAI14 antibody

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(2 Publications)

Rabbit Polyclonal RAI14 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RAI14 aa 1-50.

View Alternative Names

KIAA1334, NORPEG, RAI14, Ankycorbin, Ankyrin repeat and coiled-coil structure-containing protein, Novel retinal pigment epithelial cell protein, Retinoic acid-induced protein 14

2 Images
Immunoprecipitation - Anti-RAI14 antibody (AB241499)
  • IP

Supplier Data

Immunoprecipitation - Anti-RAI14 antibody (AB241499)

RAI14 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241499 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241499 at 1 μg/ml.

Lane 1 : ab241499 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-RAI14 antibody (ab241499)

Predicted band size: 110 kDa

false

Western blot - Anti-RAI14 antibody (AB241499)
  • WB

Supplier Data

Western blot - Anti-RAI14 antibody (AB241499)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-RAI14 antibody (ab241499) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate at 50 µg

Predicted band size: 110 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human RAI14 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9P0K7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was affinity purified using an epitope specific to RAI14 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAI14 also known as Ankycorbin or Norpeg is a protein that plays a role in cytoskeletal organization and cellular architecture. The protein has a molecular mass of approximately 110 kDa. Researchers have found RAI14 to be expressed in several tissues including testis heart and retina. In these tissues RAI14 functions to maintain cell structure and stability by interacting with actin filaments which are important components of the cell cytoskeleton. This interaction suggests RAI14’s involvement in regulating actin dynamics.
Biological function summary

Researchers believe RAI14 contributes to cellular processes by participating in complex formation with other proteins like actin. It modulates the arrangement of the actin cytoskeleton which affects cell shape movement and mechanical stability. The involvement of RAI14 in actin dynamics indicates its role in providing cellular elasticity and adaptability important for cells undergoing morphological changes. Studies also suggest that RAI14 might influence processes like cell adhesion which is key for maintaining tissue integrity.

Pathways

Scientists link RAI14 to actin-related pathways and cellular morphogenesis. It works alongside proteins such as filamin A and vinculin that interact with the actin cytoskeleton to manage cell shape and mechanical responses. RAI14’s function in these pathways is important for understanding how cells migrate and how they respond to mechanical stress. These processes are important for tissue development and repair highlighting the significance of RAI14 in maintaining cellular homeostasis.

Scientists have associated RAI14 with conditions affecting cell structure and motility. For instance RAI14 dysregulation has been observed in certain types of cancer where changes in cell adhesion and migration contribute to tumor progression. Additionally abnormalities in the expression or function of actin-related proteins like RAI14 can also lead to retinal diseases due to its expression in retinal tissue. These connections highlight the importance of RAI14 in understanding and potentially targeting cytoskeletal disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in actin regulation at the ectoplasmic specialization, a type of cell junction specific to testis. Important for establishment of sperm polarity and normal spermatid adhesion. May also promote integrity of Sertoli cell tight junctions at the blood-testis barrier.
See full target information RAI14
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 15:1613458 PubMed40823069

2025

Polyamine metabolism related gene index prediction of prognosis and immunotherapy response in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ruoya Wang,Shouliang Cai,Qing Gao,Yidong Chen,Xue Han,Fangjian Shang,Chunyan Liang,Guolian Zhu,Bo Chen

International journal of molecular sciences 23: PubMed36233342

2022

RAI14 Promotes Melanoma Progression by Regulating the FBXO32/c-MYC Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Xu,Pengfei Shi,Fanwei Xia,Xuan Zhao,Junfan Chen,Rui Geng,Hongjuan Cui,Liqun Yang
View all publications
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Product promise

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