AB248831

Anti-RAI14 antibody [EPR8518] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal RAI14 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

KIAA1334, NORPEG, RAI14, Ankycorbin, Ankyrin repeat and coiled-coil structure-containing protein, Novel retinal pigment epithelial cell protein, Retinoic acid-induced protein 14

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAI14 antibody [EPR8518] - BSA and Azide free (AB248831)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAI14 antibody [EPR8518] - BSA and Azide free (AB248831)

This data was developed using ab137118, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling RAI14 with ab137118 antibody at a dilution of 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAI14 antibody [EPR8518] - BSA and Azide free (AB248831)

This data was developed using ab137118, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human testis tissue labelling RAI14 with ab137118 antibody at a dilution of 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-RAI14 antibody [EPR8518] - BSA and Azide free (AB248831)

This data was developed using ab137118, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RAI14 antibody [EPR8518] (<a href='/en-us/products/primary-antibodies/rai14-antibody-epr8518-ab137118'>ab137118</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 110 kDa

Observed band size: 110 kDa

false

Unconjugated

Anti-RAI14 antibody [EPR8518]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8518

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab248831 is the carrier-free version of ab137118.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAI14 also known as Ankycorbin or Norpeg is a protein that plays a role in cytoskeletal organization and cellular architecture. The protein has a molecular mass of approximately 110 kDa. Researchers have found RAI14 to be expressed in several tissues including testis heart and retina. In these tissues RAI14 functions to maintain cell structure and stability by interacting with actin filaments which are important components of the cell cytoskeleton. This interaction suggests RAI14’s involvement in regulating actin dynamics.

Biological function summary

Researchers believe RAI14 contributes to cellular processes by participating in complex formation with other proteins like actin. It modulates the arrangement of the actin cytoskeleton which affects cell shape movement and mechanical stability. The involvement of RAI14 in actin dynamics indicates its role in providing cellular elasticity and adaptability important for cells undergoing morphological changes. Studies also suggest that RAI14 might influence processes like cell adhesion which is key for maintaining tissue integrity.

Pathways

Scientists link RAI14 to actin-related pathways and cellular morphogenesis. It works alongside proteins such as filamin A and vinculin that interact with the actin cytoskeleton to manage cell shape and mechanical responses. RAI14’s function in these pathways is important for understanding how cells migrate and how they respond to mechanical stress. These processes are important for tissue development and repair highlighting the significance of RAI14 in maintaining cellular homeostasis.

Scientists have associated RAI14 with conditions affecting cell structure and motility. For instance RAI14 dysregulation has been observed in certain types of cancer where changes in cell adhesion and migration contribute to tumor progression. Additionally abnormalities in the expression or function of actin-related proteins like RAI14 can also lead to retinal diseases due to its expression in retinal tissue. These connections highlight the importance of RAI14 in understanding and potentially targeting cytoskeletal disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in actin regulation at the ectoplasmic specialization, a type of cell junction specific to testis. Important for establishment of sperm polarity and normal spermatid adhesion. May also promote integrity of Sertoli cell tight junctions at the blood-testis barrier.

See full target information RAI14

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RAI14 antibody [EPR8518] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAI14 antibody [EPR8518] - BSA and Azide free (AB248831)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAI14 antibody [EPR8518] - BSA and Azide free (AB248831)

Western blot - Anti-RAI14 antibody [EPR8518] - BSA and Azide free (AB248831)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise