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AB76465

Anti-RAIDD antibody [EPR1639]

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(2 Publications)

Rabbit Recombinant Monoclonal RAIDD antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

RAIDD, CRADD, Death domain-containing protein CRADD, Caspase and RIP adapter with death domain, RIP-associated protein with a death domain

1 Images
Western blot - Anti-RAIDD antibody [EPR1639] (AB76465)
  • WB

Unknown

Western blot - Anti-RAIDD antibody [EPR1639] (AB76465)

All lanes:

Western blot - Anti-RAIDD antibody [EPR1639] (ab76465) at 1/5000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

MCF7 cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 23 kDa

Observed band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1639

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB52621

Anti-RAIDD antibody [EPR1640Y]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAIDD antibody [EPR1640Y] (AB52621)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB300617

Anti-RIP antibody [EPR24883-85]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry - Anti-RIP antibody [EPR24883-85] (AB300617)

  • 4
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB179520

Anti-Caspase-2 antibody [EPR16796]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-Caspase-2 antibody [EPR16796] (AB179520)

  • 0
  • 0
Primary Antibodies

AB75846

Anti-TRIB3 antibody [EPR3151Y]

RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-TRIB3 antibody [EPR3151Y] (AB75846)

  • 5
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAIDD also known as RIP-associated ICH-1/CED-3-homologous protein with a death domain (RIPDD) is an adaptor protein with a molecular mass of approximately 22 kDa. It is important for the formation of multiprotein signaling complexes. RAIDD contains a caspase recruitment domain and a death domain which facilitate its function in apoptotic pathways. It expresses in various tissues with a significant presence in immune and neural tissues where it has roles in regulating cell death and inflammation responses.
Biological function summary

RAIDD interacts closely with other proteins to regulate apoptosis. It forms part of the death-inducing signaling complex (DISC) a structure that recruits and activates initiator caspases like caspase-2. By acting as a bridge between receptor-interacting protein (RIP) and caspases RAIDD transmits signals that lead to programmed cell death. This regulation is central to cellular homeostasis and responses to damaging stimuli.

Pathways

RAIDD stands as a significant player in the apoptotic and inflammatory pathways. Apoptosis is well-documented where RAIDD serves as a connecting link to caspase-2 activation through the DISC. The second pathway of relevance is the TNF-receptor signaling pathway where RAIDD associates with RIP to influence downstream signaling events affecting expression of genes involved in immune response and cell cycle control.

RAIDD's role in apoptosis and inflammation connects it to cancer and neurodegenerative diseases. In cancer RAIDD is involved in pathways that regulate tumor cell survival and death. Its interaction with proteins like caspase-2 can impact the fate of cancer cells potentially leading to abnormal cell proliferation or apoptosis resistance. In neurodegenerative conditions like Alzheimer's disease RAIDD's association with pro-inflammatory pathways and cell death mechanisms implicates it in neural cell injury and disease progression often in conjunction with RIP and caspases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein that associates with PIDD1 and the caspase CASP2 to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis (PubMed : 15073321, PubMed : 16652156, PubMed : 17159900, PubMed : 17289572, PubMed : 9044836). Also recruits CASP2 to the TNFR-1 signaling complex through its interaction with RIPK1 and TRADD and may play a role in the tumor necrosis factor-mediated signaling pathway (PubMed : 8985253).
See full target information CRADD
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 10:102 PubMed30718480

2019

Pigment epithelium-derived factor mediates retinal ganglion cell neuroprotection by suppression of caspase-2.

Applications

Unspecified application

Species

Unspecified reactive species

Vasanthy Vigneswara,Zubair Ahmed

American journal of human genetics 99:1117-1129 PubMed27773430

2016

Mutations in CRADD Result in Reduced Caspase-2-Mediated Neuronal Apoptosis and Cause Megalencephaly with a Rare Lissencephaly Variant.

Applications

Unspecified application

Species

Unspecified reactive species

Nataliya Di Donato,Ying Y Jean,A Murat Maga,Briana D Krewson,Alison B Shupp,Maria I Avrutsky,Achira Roy,Sarah Collins,Carissa Olds,Rebecca A Willert,Agnieszka M Czaja,Rachel Johnson,Jessi A Stover,Steven Gottlieb,Deborah Bartholdi,Anita Rauch,Amy Goldstein,Victoria Boyd-Kyle,Kimberly A Aldinger,Ghayda M Mirzaa,Anke Nissen,Karlla W Brigatti,Erik G Puffenberger,Kathleen J Millen,Kevin A Strauss,William B Dobyns,Carol M Troy,Robert N Jinks
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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