AB52621

Anti-RAIDD antibody [EPR1640Y]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal RAIDD antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

RAIDD, CRADD, Death domain-containing protein CRADD, Caspase and RIP adapter with death domain, RIP-associated protein with a death domain

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAIDD antibody [EPR1640Y] (AB52621)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAIDD antibody [EPR1640Y] (AB52621)

ab52621 at a 1/50 dilution staining Human RAIDD in heart muscle using Immunohistochemistry Paraffin embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

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Western blot - Anti-RAIDD antibody [EPR1640Y] (AB52621)

All lanes:

Western blot - Anti-RAIDD antibody [EPR1640Y] (ab52621) at 1/1000 dilution

All lanes:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 23 kDa

Observed band size: 23 kDa

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CiteAb

Western blot - Anti-RAIDD antibody [EPR1640Y] (AB52621)

RAIDD western blot using anti-RAIDD antibody [EPR1640Y] ab52621. Publication image and figure legend from Jelínek, M., Balusikova, K., et al., 2013, Cancer Cell Int, PubMed 23672670.

ab52621 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab52621 please see the product overview.

Effect of the inhibition of RAIDD expression on the growth and survival of SK-BR-3 and MCF-7 cells after paclitaxel treatment. (A) Efficiency of RAIDD suppression by two employed specific siRNA, i.e. A and B, in SK-BR-3 and MCF-7 cells is shown. Levels of RAIDD were determined using western blot analysis and relevant antibodies (see “Materials and Methods”). Actin levels were used to confirm equal protein loading. (B) The effect of specific RAIDD siRNA on the growth and survival of SK-BR-3 and MCF-7 cells after paclitaxel (T) treatment (100 nM for SK-BR-3 cells and 300 nM for MCF-7 cells) is presented. The cells were seeded at 7 × 103 cells/200 μl of medium per well and prepared as described (see “Materials and Methods”). After 0, 48 and 96 h of incubation, the number of living cells was determined (see “Materials and Methods”). Each column represents the mean of 2 separate cultures ± SEM.

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1640Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAIDD also known as RIP-associated ICH-1/CED-3-homologous protein with a death domain (RIPDD) is an adaptor protein with a molecular mass of approximately 22 kDa. It is important for the formation of multiprotein signaling complexes. RAIDD contains a caspase recruitment domain and a death domain which facilitate its function in apoptotic pathways. It expresses in various tissues with a significant presence in immune and neural tissues where it has roles in regulating cell death and inflammation responses.

Biological function summary

RAIDD interacts closely with other proteins to regulate apoptosis. It forms part of the death-inducing signaling complex (DISC) a structure that recruits and activates initiator caspases like caspase-2. By acting as a bridge between receptor-interacting protein (RIP) and caspases RAIDD transmits signals that lead to programmed cell death. This regulation is central to cellular homeostasis and responses to damaging stimuli.

Pathways

RAIDD stands as a significant player in the apoptotic and inflammatory pathways. Apoptosis is well-documented where RAIDD serves as a connecting link to caspase-2 activation through the DISC. The second pathway of relevance is the TNF-receptor signaling pathway where RAIDD associates with RIP to influence downstream signaling events affecting expression of genes involved in immune response and cell cycle control.

RAIDD's role in apoptosis and inflammation connects it to cancer and neurodegenerative diseases. In cancer RAIDD is involved in pathways that regulate tumor cell survival and death. Its interaction with proteins like caspase-2 can impact the fate of cancer cells potentially leading to abnormal cell proliferation or apoptosis resistance. In neurodegenerative conditions like Alzheimer's disease RAIDD's association with pro-inflammatory pathways and cell death mechanisms implicates it in neural cell injury and disease progression often in conjunction with RIP and caspases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adapter protein that associates with PIDD1 and the caspase CASP2 to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis (PubMed : 15073321, PubMed : 16652156, PubMed : 17159900, PubMed : 17289572, PubMed : 9044836). Also recruits CASP2 to the TNFR-1 signaling complex through its interaction with RIPK1 and TRADD and may play a role in the tumor necrosis factor-mediated signaling pathway (PubMed : 8985253).

See full target information CRADD

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular metabolism 16:139-149 PubMed29935921

2018

Loss of Tbk1 kinase activity protects mice from diet-induced metabolic dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria H Cruz,Emily N Arner,Katherine W Wynne,Philipp E Scherer,Rolf A Brekken

American journal of human genetics 99:1117-1129 PubMed27773430

2016

Mutations in CRADD Result in Reduced Caspase-2-Mediated Neuronal Apoptosis and Cause Megalencephaly with a Rare Lissencephaly Variant.

Applications

Unspecified application

Species

Unspecified reactive species

Nataliya Di Donato,Ying Y Jean,A Murat Maga,Briana D Krewson,Alison B Shupp,Maria I Avrutsky,Achira Roy,Sarah Collins,Carissa Olds,Rebecca A Willert,Agnieszka M Czaja,Rachel Johnson,Jessi A Stover,Steven Gottlieb,Deborah Bartholdi,Anita Rauch,Amy Goldstein,Victoria Boyd-Kyle,Kimberly A Aldinger,Ghayda M Mirzaa,Anke Nissen,Karlla W Brigatti,Erik G Puffenberger,Kathleen J Millen,Kevin A Strauss,William B Dobyns,Carol M Troy,Robert N Jinks

American journal of translational research 6:538-47 PubMed25360218

2014

Regulation of CRADD-caspase 2 cascade by histone deacetylase 1 in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Shen,Wanfen Tang,Jie Sun,Lifeng Feng,Hongchuan Jin,Xian Wang

Cancer cell international 13:42 PubMed23672670

2013

Caspase-2 is involved in cell death induction by taxanes in breast cancer cells.

Applications

Species

Unspecified reactive species

Michael Jelínek,Kamila Balušíková,Dana Kopperová,Vlasta Nĕmcová-Fürstová,Jan Šrámek,Julie Fidlerová,Ilaria Zanardi,Iwao Ojima,Jan Kovář

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RAIDD antibody [EPR1640Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAIDD antibody [EPR1640Y] (AB52621)

Western blot - Anti-RAIDD antibody [EPR1640Y] (AB52621)

Western blot - Anti-RAIDD antibody [EPR1640Y] (AB52621)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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