AB236314

Anti-RALA antibody

Name: Anti-RALA antibody (ab236314) | Abcam
Brand: Abcam Limited
SKU: AB236314

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(2 Publications)

Rabbit Polyclonal RALA antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RALA aa 1 to C-terminus.

View Alternative Names

RAL, RALA, Ras-related protein Ral-A

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RALA antibody (AB236314)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RALA antibody (AB236314)

Paraffin-embedded human adrenal gland tissue stained for RALA using ab236314 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-RALA antibody (AB236314)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RALA antibody (AB236314)

4% formaldehyde-fixed, 0.2 Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling RALA using ab236314 (green) at 1/100 dilution in ICC/IF.

Cells were blocked in 10% normal goat serum and incubated with primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (H+L). Counter stained with DAPI (blue).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RALA aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P11233

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/20 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Saguinus oedipus": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RALA also known as Ras-related protein Ral-A or RalA is a small GTPase involved in a variety of cellular processes. It has a molecular mass of approximately 23 kDa. RALA switches between inactive GDP-bound and active GTP-bound states facilitating the transduction of signals within the cell. This protein is found in a range of tissues with expression in the brain lung and kidney being notable. The ability of RALA to shuttle between membrane compartments highlights its mechanical versatility in cellular signaling.

Biological function summary

The interaction of RALA with effector proteins drives key processes like vesicle trafficking cytoskeletal dynamics and gene expression. RALA forms part of a signaling complex in its active state that mediates these functions. This protein is vital in the neurotransmitter release ensuring efficient communication between neurons. Additionally RALA impacts cell movement by influencing actin filament organization showing its role in cell motility.

Pathways

The engagement of RALA in Ral signaling and the phosphatidylinositol 3-kinase (PI3K) pathway showcases its importance. In Ral signaling RALA interacts closely with proteins like RalBP1 influencing endocytosis and exocytosis activities. In the PI3K pathway RALA helps regulate cell proliferation and survival showing interactions with proteins such as AKT1. These pathways highlight the integration of RALA into broad signal transduction networks important for cellular homeostasis.

The altered regulation of RALA links it to cancer and neuronal disorders. In cancer the dysregulation of RALA can drive tumorigenesis often acting alongside proteins like KRAS in promoting oncogenic signaling. In neuronal disorders improper functioning of RALA has been associated with synaptic dysfunctions potentially affecting proteins like Synapsin I which are important to synaptic vesicle trafficking. Understanding RALA's role in these contexts helps in developing targeted therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors (PubMed : 18756269, PubMed : 19306925, PubMed : 20005108, PubMed : 21822277, PubMed : 30500825). Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling (PubMed : 20005108). Key regulator of LPAR1 signaling and competes with GRK2 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells (PubMed : 19306925). During mitosis, supports the stabilization and elongation of the intracellular bridge between dividing cells. Cooperates with EXOC2 to recruit other components of the exocyst to the early midbody (PubMed : 18756269). During mitosis, also controls mitochondrial fission by recruiting to the mitochondrion RALBP1, which mediates the phosphorylation and activation of DNM1L by the mitotic kinase cyclin B-CDK1 (PubMed : 21822277).

See full target information RALA

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 20:1004-1023 PubMed38250155

2024

Inactivation of MST1/2 Controls Macrophage Polarization to Affect Macrophage-Related Disease via YAP and Non-YAP Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Yina An,Shuyu Tan,Pu Zhang,Jingjing Yang,Kezhi Wang,Ruicheng Zheng,Lu Qiao,Yang Wang,Yanjun Dong

Frontiers in immunology 13:1046121 PubMed36483553

2022

Immune and non-immune cell subtypes identify novel targets for prognostic and therapeutic strategy: A study based on intratumoral heterogenicity analysis of multicenter scRNA-seq datasets in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyu Fan,Jian Lu,Delei Niu,Yue Zhang,Bin Wang,Bei Zhang,Zugui Zhang,Xinjiai He,Nan Peng,Biao Li,Huilong Fang,Zheng Gong,Li Zhang

View all publications

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Product promise

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Anti-RALA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RALA antibody (AB236314)

Immunocytochemistry/ Immunofluorescence - Anti-RALA antibody (AB236314)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise