AB223479

Anti-RALB antibody

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(3 Publications)

Rabbit Polyclonal RALB antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Ras-related protein Ral-B.

View Alternative Names

Ras-related protein Ral-B, RALB

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RALB antibody (AB223479)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RALB antibody (AB223479)

Paraffin-embedded human ovarian cancer tissue stained for RALB using ab223479 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Ras-related protein Ral-B.

P11234

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RALB also known as Ras-related protein Ral-B is a member of the small GTPase superfamily with a mass of about 23 kDa. It functions mechanically as a molecular switch cycling between an active GTP-bound state and an inactive GDP-bound state. Its principally known roles involve endocytosis exocytosis and regulation of cell proliferation. RALB expresses in multiple tissues with higher levels observed in the brain lungs and pancreas.

Biological function summary

RALB plays important roles in membrane trafficking and cellular processes. It forms complexes with exocyst components facilitating the tethering and fusion of vesicles with target membranes. RALB interacts with various effector proteins such as Sec5 and RalBP1 to modulate cytoskeletal dynamics and vesicle trafficking. Through these activities RALB influences cell migration cell division and even tumorigenesis.

Pathways

RALB contributes significantly to the regulation of the Ras signaling pathway and the exocyst pathway. It interacts closely with the Ras family of proteins and can be activated by signals upstream in these pathways. In particular RALB's role within the exocyst pathway facilitates the targeting and delivery of secretory vesicles impacting processes like synaptic transmission and immune response. RALB also links with proteins like RALA and RAS coordinating diverse cellular activities.

RALB links to cancer progression and invasion due to its role in cell migration and proliferation. Its activity relates to conditions like pancreatic and bladder cancer where it can affect tumor growth and metastasis. Studies have shown that RALB activity influences autophagy and apoptotic pathways interacting with proteins such as mTOR and p53 which are key players in cancer development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking (PubMed : 10393179, PubMed : 17875936, PubMed : 18756269). Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles (By similarity). Required both to stabilize the assembly of the exocyst complex and to localize functional exocyst complexes to the leading edge of migrating cells (By similarity). Required for suppression of apoptosis (PubMed : 17875936). In late stages of cytokinesis, upon completion of the bridge formation between dividing cells, mediates exocyst recruitment to the midbody to drive abscission (PubMed : 18756269). Involved in ligand-dependent receptor mediated endocytosis of the EGF and insulin receptors (PubMed : 10393179).

See full target information Ras-related protein Ral-B

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Breast cancer research : BCR 23:65 PubMed34118960

2021

The small G-protein RalA promotes progression and metastasis of triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Katie A Thies,Matthew W Cole,Rachel E Schafer,Jonathan M Spehar,Dillon S Richardson,Sarah A Steck,Manjusri Das,Arthur W Lian,Alo Ray,Reena Shakya,Sue E Knoblaugh,Cynthia D Timmers,Michael C Ostrowski,Arnab Chakravarti,Gina M Sizemore,Steven T Sizemore

Archives of biochemistry and biophysics 704:108719 PubMed33290747

2020

MicroRNA-139 inhibits pancreatic-cancer carcinogenesis by suppressing RalB via the Ral/RAC/PI3K pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Wang,Yan Zheng,Qiao Chen,Yongmei Dai,Ting Li

JCI insight 5: PubMed30938713

2019

Axl-mediated activation of TBK1 drives epithelial plasticity in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria H Cruz,Emily N Arner,Wenting Du,Alberto E Bremauntz,Rolf A Brekken

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-RALB antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RALB antibody (AB223479)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise