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Rabbit Recombinant Monoclonal RALBP1 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

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Images

Western blot - Anti-RALBP1 antibody [EPR6472] (AB133549), expandable thumbnail
  • Western blot - Anti-RALBP1 antibody [EPR6472] (AB133549), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-RALBP1 antibody [EPR6472] (AB133549), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RALBP1 antibody [EPR6472] (AB133549), expandable thumbnail
  • OI-RD Scanning - Anti-RALBP1 antibody [EPR6472] (AB133549), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Not recommended
Tested
Mouse
Predicted
Not recommended
Predicted
Not recommended
Predicted
Rat
Predicted
Not recommended
Predicted
Not recommended
Predicted

Tested
Tested

Species
Human
Dilution info
1/250 - 1/500
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10000 - 1/50000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/1000
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Multifunctional protein that functions as a downstream effector of RALA and RALB (PubMed:7673236). As a GTPase-activating protein/GAP can inactivate CDC42 and RAC1 by stimulating their GTPase activity (PubMed:7673236). As part of the Ral signaling pathway, may also regulate ligand-dependent EGF and insulin receptors-mediated endocytosis (PubMed:10910768, PubMed:12775724). During mitosis, may act as a scaffold protein in the phosphorylation of EPSIN/EPN1 by the mitotic kinase cyclin B-CDK1, preventing endocytosis during that phase of the cell cycle (PubMed:12775724). During mitosis, also controls mitochondrial fission as an effector of RALA (PubMed:21822277). Recruited to mitochondrion by RALA, acts as a scaffold to foster the mitotic kinase cyclin B-CDK1-mediated phosphorylation and activation of DNM1L (PubMed:21822277). Could also function as a primary ATP-dependent active transporter for glutathione conjugates of electrophiles. May also actively catalyze the efflux of a wide range of substrates including xenobiotics like doxorubicin (DOX) contributing to cell multidrug resistance.

Alternative names

RLIP, RLIP1, RLIP76, RALBP1, RalA-binding protein 1, RalBP1, 76 kDa Ral-interacting protein, Dinitrophenyl S-glutathione ATPase, Ral-interacting protein 1, DNP-SG ATPase

Recommended products

Rabbit Recombinant Monoclonal RALBP1 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR6472
Purification technique
Affinity purification Protein A
Dissociation constant
5.42 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RALBP1 also known as ralA binding protein 1 or RLIP76 is a multifunctional protein involved in various cellular processes. It has a molecular mass of approximately 76 kDa. RALBP1 often associates with cell membranes and is expressed ubiquitously in different tissues indicating its widespread role in cellular functioning. The protein interacts with small GTPases such as RalA playing critical roles in signal transduction processes.

Biological function summary

RALBP1 serves as an effector protein involved in several cellular activities including endocytosis and transport of glutathione conjugates. It functions as part of a complex with partner proteins facilitating the movement of substrates across cellular membranes. This role is essential for maintaining cellular homeostasis as it helps to eliminate toxic compounds and regulate intracellular signals.

Pathways

RALBP1 participates in the Rho family small GTPase signaling and the Ras signaling pathways. Within these pathways the protein coordinates with RalA and other GTPases to control the trafficking of vesicles and stress response mechanisms. Through these interactions RALBP1 plays an important role in cytoskeletal reorganization and cellular response to extracellular cues.

Associated diseases and disorders

RALBP1 has associations with cancer and metabolic conditions. Alterations in its function or expression levels may contribute to oncogenic processes as RALBP1 interacts with factors like the epidermal growth factor receptor (EGFR) which is often dysregulated in cancerous cells. Moreover its role in detoxification processes implicates RALBP1 in metabolic disorders potentially affecting the body's capability to manage oxidative stress.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-RALBP1 antibody [EPR6472] (ab133549), expandable thumbnail

    Western blot - Anti-RALBP1 antibody [EPR6472] (ab133549)

    Lanes 1-3: Merged signal (red and green). Green - ab133549 observed at 95 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.

    ab133549 Anti-RALBP1 antibody [EPR6472] was shown to specifically react with PDE10A in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human RALBP1 knockout HeLa cell line ab265404 (knockout cell lysate Human RALBP1 knockout HeLa cell lysate ab258167) was used. Wild-type and PDE10A knockout samples were subjected to SDS-PAGE. ab133549 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-RALBP1 antibody [EPR6472] (ab133549) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: RALBP1 knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human RALBP1 knockout HeLa cell line (Human RALBP1 knockout HeLa cell line ab265404)

    Lane 3: Daudi cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 76 kDa

    Observed band size: 95 kDa

  • Western blot - Anti-RALBP1 antibody [EPR6472] (ab133549), expandable thumbnail

    Western blot - Anti-RALBP1 antibody [EPR6472] (ab133549)

    All lanes: Western blot - Anti-RALBP1 antibody [EPR6472] (ab133549) at 1/10000 dilution

    Lane 1: MCF7 cell lysate at 10 µg

    Lane 2: HT-29 cell lysate at 10 µg

    Lane 3: HeLa cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 76 kDa

    Observed band size: 95 kDa

  • Flow Cytometry (Intracellular) - Anti-RALBP1 antibody [EPR6472] (ab133549), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-RALBP1 antibody [EPR6472] (ab133549)

    Overlay histogram showing HEK293 cells stained with ab133549 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133549, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RALBP1 antibody [EPR6472] (ab133549), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RALBP1 antibody [EPR6472] (ab133549)

    Immunohistochemical analysis of paraffin embedded Human lung tissue labelling RALBP1 with ab133549 antibody at a dilution of 1/250.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-RALBP1 antibody [EPR6472] (ab133549), expandable thumbnail

    OI-RD Scanning - Anti-RALBP1 antibody [EPR6472] (ab133549)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

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