AB156575

Anti-RAMP1 antibody [EPR10867]

Name: Anti-RAMP1 antibody [EPR10867] Rabbit monoclonal (ab156575) | Abcam
Brand: Abcam Limited
SKU: AB156575
Price: 325 USD
Availability: InStock
Rating: 4 (3 reviews)

RabMAb
Recombinant
What is this?

(3 Reviews)

(17 Publications)

Rabbit Recombinant Monoclonal RAMP1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 17 publications.

View Alternative Names

Receptor activity-modifying protein 1, Calcitonin-receptor-like receptor activity-modifying protein 1, CRLR activity-modifying protein 1, RAMP1

4 Images

Lab

Western blot - Anti-RAMP1 antibody [EPR10867] (AB156575)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RAMP1 antibody [EPR10867] (ab156575) at 1/1000 dilution

All lanes:

Human uterus lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 17 kDa

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Unknown

Western blot - Anti-RAMP1 antibody [EPR10867] (AB156575)

All lanes:

Western blot - Anti-RAMP1 antibody [EPR10867] (ab156575) at 1/1000 dilution

Lane 1:

Human uterus lysate at 10 µg

Lane 2:

Human fetal brain lysate at 10 µg

Lane 3:

Caco-2 (Human colorectal adenocarcinoma cell line) cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 17 kDa

false

Lab

Western blot - Anti-RAMP1 antibody [EPR10867] (AB156575)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RAMP1 antibody [EPR10867] (ab156575) at 1/5000 dilution

Lane 1:

Mouse brain lysates at 15 µg

Lane 2:

Rat brain lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 17 kDa

false

CiteAb

Western blot - Anti-RAMP1 antibody [EPR10867] (AB156575)

RAMP1 western blot using anti-RAMP1 antibody [EPR10867] ab156575. Publication image and figure legend from Muschter, D., Beiderbeck, A. S., et al., 2019, Int J Mol Sci, PubMed 30682804.

ab156575 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab156575 please see the product overview.

The impact of mechanical loading on sensory neuropeptide receptor protein expression of RAW264.7 cells. Receptor protein expression of NK1R (A), CRLR (B) and Ramp1 (C) was analyzed using the Western Blot of lysates from cells loaded for 4 h/day on 1, 2 and 3 consecutive days. Expression of β-actin served as endogenous loading control (=100% line). Mann–Whitney test * p < 0.05; ** p < 0.01; *** p < 0.001. n = 7–8; (D) Representative Western Blot pictures for the CRLR (~53 kDa), NK1R (~46 kDa), Ramp1 (~17 kDa) and β-actin (~37 kDa, endogenous control) of control cells and cells loaded for 1, 2 and 3 consecutive days (presenting 2 lanes for each condition). NK1R—neurokinin receptor 1, CRLR—calcitonin receptor-like receptor, Ramp1—receptor activity modifying protein 1.

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Carrier free

Anti-RAMP1 antibody [EPR10867] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10867

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Accessory protein that interacts with and modulates the function of G-protein coupled receptors including calcitonin gene-related peptide type 1 receptor (CALCRL) and calcitonin receptor (CALCR) (PubMed : 33602864, PubMed : 9620797, PubMed : 35324283, PubMed : 38603770). Required for the transport of CALCRL to the plasma membrane (PubMed : 9620797). Together with CALCRL, form the receptor complex for the calcitonin gene-related peptides CGRP1/CALCA and CGRP2/CALCB (PubMed : 33602864, PubMed : 9620797). Together with CALCR, form the AMYR1 receptor complex for amylin/IAPP and CGRP1/CALCA (PubMed : 35324283, PubMed : 38603770).

See full target information RAMP1

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceuticals (Basel, Switzerland) 17: PubMed39338366

2024

Intermedin Alleviates Diabetic Cardiomyopathy by Up-Regulating CPT-1β through Activation of the Phosphatidyl Inositol 3 Kinase/Protein Kinase B Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhao,Ling Han,Ya-Rong Zhang,Shi-Meng Liu,Deng-Ren Ji,Rui Wang,Yan-Rong Yu,Mo-Zhi Jia,San-Bao Chai,Hui-Fang Tang,Wei Huang,Yong-Fen Qi

Heliyon 10:e35862 PubMed39224276

2024

Spinal RAMP1-mediated neuropathic pain sensitisation in the aged mice through the modulation of CGRP-CRLR pain signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Qin,Xuemei Chen,Zhangjie Yu,Xiaoxin Zhou,Yihao Wang,Qi Li,Wanbing Dai,Yizhe Zhang,Sa Wang,Yinghui Fan,Jie Xiao,Diansan Su,Yingfu Jiao,Weifeng Yu

Journal of clinical and translational hepatology 12:357-370 PubMed38638379

2024

RAMP1 Protects Hepatocytes against Ischemia-reperfusion Injury by Inhibiting the ERK/YAP Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yongsheng Tang,Zenan Yuan,Xu Lu,Yingqiu Song,Shuguang Zhu,Chunhui Qiu,Qi Zhang,Binsheng Fu,Changchang Jia,Hua Li

International journal of molecular sciences 23: PubMed36555690

2022

Characterization of Antibodies against Receptor Activity-Modifying Protein 1 (RAMP1): A Cautionary Tale.

Applications

Unspecified application

Species

Unspecified reactive species

Erica R Hendrikse,Tayla A Rees,Zoe Tasma,Michael L Garelja,Andrew Siow,Paul W R Harris,John B Pawlak,Kathleen M Caron,Elizabeth S Blakeney,Andrew F Russo,Levi P Sowers,Thomas A Lutz,Christelle Le Foll,Christopher S Walker,Debbie L Hay

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 9:e2202620 PubMed36047655

2022

Sensory Nerve Maintains Intervertebral Disc Extracellular Matrix Homeostasis Via CGRP/CHSY1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Hu,Xiao Lv,Leixin Wei,Yunhao Wang,Genjiang Zheng,Chen Yang,Fazhi Zang,Jianxi Wang,Jing Li,Xiaodong Wu,Zhihao Yue,Qiangqiang Xiao,Zengwu Shao,Wen Yuan,Jinsong Li,Peng Cao,Chen Xu,Huajiang Chen

Cell communication and signaling : CCS 20:52 PubMed35413847

2022

Receptor activity-modifying protein 1 regulates mouse skin fibroblast proliferation via the Gαi3-PKA-CREB-YAP axis.

Applications

Unspecified application

Species

Unspecified reactive species

Siyuan Yin,Ru Song,Jiaxu Ma,Chunyan Liu,Zhenjie Wu,Guoqi Cao,Jian Liu,Guang Zhang,Huayu Zhang,Rui Sun,Aoyu Chen,Yibing Wang

Nature communications 13:646 PubMed35115501

2022

Schwann cell endosome CGRP signals elicit periorbital mechanical allodynia in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Francesco De Logu,Romina Nassini,Alan Hegron,Lorenzo Landini,Dane D Jensen,Rocco Latorre,Julia Ding,Matilde Marini,Daniel Souza Monteiro de Araujo,Paulina Ramírez-Garcia,Michael Whittaker,Jeffri Retamal,Mustafa Titiz,Alessandro Innocenti,Thomas P Davis,Nicholas Veldhuis,Brian L Schmidt,Nigel W Bunnett,Pierangelo Geppetti

Frontiers in immunology 12:722884 PubMed34512650

2021

Substance P and Alpha-Calcitonin Gene-Related Peptide Differentially Affect Human Osteoarthritic and Healthy Chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Sabine Stöckl,Annett Eitner,Richard J Bauer,Matthias König,Brian Johnstone,Susanne Grässel

Frontiers in pharmacology 12:685631 PubMed34234676

2021

Salmon Calcitonin Attenuates Some Behavioural Responses to Nicotine in Male Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Cajsa Aranäs,Jesper Vestlund,Sarah Witley,Christian E Edvardsson,Aimilia Lydia Kalafateli,Elisabet Jerlhag

Journal of the Endocrine Society 5:bvaa199 PubMed33506161

2021

Circulating Levels of Calcitonin Gene-Related Peptide Are Lower in COVID-19 Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Ochoa-Callejero,Josune García-Sanmartín,Pablo Villoslada-Blanco,María Íñiguez,Patricia Pérez-Matute,Elisabet Pujadas,Mary E Fowkes,Rachel Brody,José A Oteo,Alfredo Martínez

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RAMP1 antibody [EPR10867]

Western blot - Anti-RAMP1 antibody [EPR10867] (AB156575)

Western blot - Anti-RAMP1 antibody [EPR10867] (AB156575)

Western blot - Anti-RAMP1 antibody [EPR10867] (AB156575)

Western blot - Anti-RAMP1 antibody [EPR10867] (AB156575)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (17)

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