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AB184571

Anti-Ramp4-2 antibody [EPR14338]

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(3 Publications)

Rabbit Recombinant Monoclonal Ramp4-2 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 3 publications.

View Alternative Names

C13orf21, SERP2, Stress-associated endoplasmic reticulum protein 2, Ribosome-associated membrane protein RAMP4-2

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Ramp4-2 antibody [EPR14338] (AB184571)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Ramp4-2 antibody [EPR14338] (AB184571)

Immunofluorescent analysis of 4% paraformaldehyde-fixed SW480 cells labeling Ramp4-2 with ab184571 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 diution. Counter stained with Dapi (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ramp4-2 antibody [EPR14338] (AB184571)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ramp4-2 antibody [EPR14338] (AB184571)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Ramp4-2 with ab184571 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Western blot - Anti-Ramp4-2 antibody [EPR14338] (AB184571)
  • WB

Supplier Data

Western blot - Anti-Ramp4-2 antibody [EPR14338] (AB184571)

All lanes:

Western blot - Anti-Ramp4-2 antibody [EPR14338] (ab184571) at 1/100000 dilution

Lane 1:

LnCaP cell lysate at 10 µg

Lane 2:

SW480 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 7 kDa

Observed band size: 7 kDa

false

Western blot - Anti-Ramp4-2 antibody [EPR14338] (AB184571)
  • WB

Supplier Data

Western blot - Anti-Ramp4-2 antibody [EPR14338] (AB184571)

All lanes:

Western blot - Anti-Ramp4-2 antibody [EPR14338] (ab184571) at 1/50000 dilution

All lanes:

293T cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 7 kDa

Observed band size: 7 kDa

false

  • Carrier free

    Anti-Ramp4-2 antibody [EPR14338] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14338

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ramp4-2 also known as Ribosome-associated membrane protein 4-2 plays a role in the endoplasmic reticulum (ER) where it assists in protein synthesis and translocation. It is a small protein with a mass of approximately 8 kDa. Ramp4-2 is widely expressed in many tissues indicating its important function in cellular activities. This protein helps facilitate the movement of nascent polypeptides across the ER membrane ensuring proper folding and function of synthesized proteins.
Biological function summary

Ramp4-2 facilitates the assembly and stabilization of proteins as part of a ribosome-nascent chain complex. It ensures efficient translocation of polypeptides into the ER lumen where they fold and mature. By being part of this complex Ramp4-2 aids in maintaining the efficient functioning of protein translocation machinery thereby supporting overall protein homeostasis in cells. Its involvement in such cellular processes highlights its essential role in maintaining proper cellular function and protein balance.

Pathways

The Ramp4-2 protein engages with several other elements in the secretory pathway particularly the translocation pathway at the ER. It interacts closely with signal recognition particle (SRP) to guide ribosome-mRNA complexes to the ER. It also has connections to the complex involving Sec61 a translocon channel which facilitates polypeptide translocation into the ER. This participation indicates its importance in the ER stress response pathway acting alongside other components to ensure proper folding and processing of secreted and membrane-bound proteins.

Ramp4-2 has links to certain pathologies where protein misfolding or ER stress plays a role. Dysregulation or mutations in proteins associated with Ramp4-2 may contribute to neurodegenerative diseases such as Alzheimer's disease given the protein's integral role in protein folding and homeostasis. Likewise it may intersect with disorders related to metabolic dysregulation as it participates in pathways important for maintaining cellular proteostasis. Ramp4-2's interactions with proteins involved in these diseases highlight its potential relevance in therapeutic research and intervention strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Interacts with target proteins during their translocation into the lumen of the endoplasmic reticulum. Protects unfolded target proteins against degradation during ER stress. May facilitate glycosylation of target proteins after termination of ER stress. May modulate the use of N-glycosylation sites on target proteins.
See full target information SERP2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 30:522 PubMed40995140

2025

ARHGEF6 downregulation as a key mediator of tumor cell apoptosis in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zhang,Dandan Zhou,Xuting Shen,Qinqin Song,Liuyang Yang,Wenbo Li,Xinxin Zhao,Wenxiang Fang,Bian Liu,Chencen Xing,Jisong Liu,Haoshu Fang,Qilun Zhang

Methods in molecular biology (Clifton, N.J.) 2845:109-126 PubMed39115661

2024

Methods for Monitoring ER-Phagy.

Applications

Unspecified application

Species

Unspecified reactive species

Marisa Di Monaco,Marian Peteri,Jin Rui Liang

eLife 13: PubMed38896445

2024

Structural analysis of the dynamic ribosome-translocon complex.

Applications

Unspecified application

Species

Unspecified reactive species

Aaron J O Lewis,Frank Zhong,Robert J Keenan,Ramanujan S Hegde
View all publications
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Product promise

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