AB250772

Anti-Ramp4-2 antibody [EPR14338] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Ramp4-2 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C13orf21, SERP2, Stress-associated endoplasmic reticulum protein 2, Ribosome-associated membrane protein RAMP4-2

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Ramp4-2 antibody [EPR14338] - BSA and Azide free (AB250772)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Ramp4-2 antibody [EPR14338] - BSA and Azide free (AB250772)

This data was developed using ab184571, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed SW480 cells labeling Ramp4-2 with ab184571 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 diution. Counter stained with Dapi (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ramp4-2 antibody [EPR14338] - BSA and Azide free (AB250772)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ramp4-2 antibody [EPR14338] - BSA and Azide free (AB250772)

This data was developed using ab184571, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Ramp4-2 with ab184571 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Ramp4-2 antibody [EPR14338] - BSA and Azide free (AB250772)

This data was developed using ab184571, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Ramp4-2 antibody [EPR14338] (<a href='/en-us/products/primary-antibodies/ramp4-2-antibody-epr14338-ab184571'>ab184571</a>) at 1/100000 dilution

Lane 1:

LnCaP cell lysate at 10 µg

Lane 2:

SW480 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 7 kDa

Observed band size: 7 kDa

false

Supplier Data

Western blot - Anti-Ramp4-2 antibody [EPR14338] - BSA and Azide free (AB250772)

This data was developed using ab184571, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Ramp4-2 antibody [EPR14338] (<a href='/en-us/products/primary-antibodies/ramp4-2-antibody-epr14338-ab184571'>ab184571</a>) at 1/50000 dilution

All lanes:

293T cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 7 kDa

Observed band size: 7 kDa

false

Unconjugated

Anti-Ramp4-2 antibody [EPR14338]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14338

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab250772 is the carrier-free version of ab184571.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ramp4-2 also known as Ribosome-associated membrane protein 4-2 plays a role in the endoplasmic reticulum (ER) where it assists in protein synthesis and translocation. It is a small protein with a mass of approximately 8 kDa. Ramp4-2 is widely expressed in many tissues indicating its important function in cellular activities. This protein helps facilitate the movement of nascent polypeptides across the ER membrane ensuring proper folding and function of synthesized proteins.

Biological function summary

Ramp4-2 facilitates the assembly and stabilization of proteins as part of a ribosome-nascent chain complex. It ensures efficient translocation of polypeptides into the ER lumen where they fold and mature. By being part of this complex Ramp4-2 aids in maintaining the efficient functioning of protein translocation machinery thereby supporting overall protein homeostasis in cells. Its involvement in such cellular processes highlights its essential role in maintaining proper cellular function and protein balance.

Pathways

The Ramp4-2 protein engages with several other elements in the secretory pathway particularly the translocation pathway at the ER. It interacts closely with signal recognition particle (SRP) to guide ribosome-mRNA complexes to the ER. It also has connections to the complex involving Sec61 a translocon channel which facilitates polypeptide translocation into the ER. This participation indicates its importance in the ER stress response pathway acting alongside other components to ensure proper folding and processing of secreted and membrane-bound proteins.

Ramp4-2 has links to certain pathologies where protein misfolding or ER stress plays a role. Dysregulation or mutations in proteins associated with Ramp4-2 may contribute to neurodegenerative diseases such as Alzheimer's disease given the protein's integral role in protein folding and homeostasis. Likewise it may intersect with disorders related to metabolic dysregulation as it participates in pathways important for maintaining cellular proteostasis. Ramp4-2's interactions with proteins involved in these diseases highlight its potential relevance in therapeutic research and intervention strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Interacts with target proteins during their translocation into the lumen of the endoplasmic reticulum. Protects unfolded target proteins against degradation during ER stress. May facilitate glycosylation of target proteins after termination of ER stress. May modulate the use of N-glycosylation sites on target proteins.

See full target information SERP2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 16:4716-4727 PubMed39678812

2024

Enhancing the radiosensitivity of colorectal cancer cells by reducing spermine synthase through promoting autophagy and DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Bin Guo,Yue-Ming Wu,Zhi-Zhao Lin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ramp4-2 antibody [EPR14338] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-Ramp4-2 antibody [EPR14338] - BSA and Azide free (AB250772)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ramp4-2 antibody [EPR14338] - BSA and Azide free (AB250772)

Western blot - Anti-Ramp4-2 antibody [EPR14338] - BSA and Azide free (AB250772)

Western blot - Anti-Ramp4-2 antibody [EPR14338] - BSA and Azide free (AB250772)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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