AB233762

Anti-Ran antibody [8D1H12]

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(2 Publications)

Mouse Monoclonal RAN antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human, Rat, African green monkey, Transfected cell lysate - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RAN.

View Alternative Names

ARA24, OK/SW-cl.81, RAN, GTP-binding nuclear protein Ran, Androgen receptor-associated protein 24, GTPase Ran, Ras-like protein TC4, Ras-related nuclear protein

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ran antibody [8D1H12] (AB233762)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ran antibody [8D1H12] (AB233762)

Paraffin-embedded human esophageal cancer tissue stained for Ran using ab233762 at 1/200 dilution in immunohistochemical analysis. DAB staining.

Flow Cytometry - Anti-Ran antibody [8D1H12] (AB233762)

Flow Cyt

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Flow Cytometry - Anti-Ran antibody [8D1H12] (AB233762)

Flow cytometric analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Ran using ab233762 at 1/200 dilution (green) compared to a negative control (red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ran antibody [8D1H12] (AB233762)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ran antibody [8D1H12] (AB233762)

Paraffin-embedded human rectum cancer tissue stained for Ran using ab233762 at 1/200 dilution in immunohistochemical analysis. DAB staining.

Western blot - Anti-Ran antibody [8D1H12] (AB233762)

WB

Supplier Data

Western blot - Anti-Ran antibody [8D1H12] (AB233762)

All lanes:

Western blot - Anti-Ran antibody [8D1H12] (ab233762) at 1/500 dilution

Lane 1:

A431 (human epidermoid carcinoma cell line) cell lysate

Lane 2:

C6 (Rat glial tumor cell line) cell lysate

Lanes 3 and 6:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

Lane 4:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 5:

COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate

Predicted band size: 24 kDa

false

Western blot - Anti-Ran antibody [8D1H12] (AB233762)

WB

Supplier Data

Western blot - Anti-Ran antibody [8D1H12] (AB233762)

All lanes:

Western blot - Anti-Ran antibody [8D1H12] (ab233762) at 1/500 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

RAN (AA: 1-216)-hIgGFc transfected HEK-293 cell lysate

Predicted band size: 24 kDa

false

Western blot - Anti-Ran antibody [8D1H12] (AB233762)

WB

Supplier Data

Western blot - Anti-Ran antibody [8D1H12] (AB233762)

Expected MW is 50.4 kDa.

All lanes:

Western blot - Anti-Ran antibody [8D1H12] (ab233762) at 1/500 dilution

All lanes:

Recombinant human Ran protein

Predicted band size: 24 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8D1H12

Isotype

IgG1

Carrier free

No

Reacts with

Rat, Human, African green monkey

Applications

Flow Cyt, WB, IHC-P

Immunogen

Recombinant Full Length Protein corresponding to Human RAN.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "African green monkey": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell lysate - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ran also known as Ras-related nuclear protein is a small GTP-binding protein with a mass of approximately 24 kDa. It localizes mainly in the nucleus and cytoplasm of cells and is highly expressed in actively dividing cells including various types of tumors. Ran plays a mechanical role in nucleocytoplasmic transport by facilitating the transport of proteins and ribonucleic acids through the nuclear pore complex. This protein belongs to the Ras superfamily of small GTPases which cycle between active GTP-bound and inactive GDP-bound states.

Biological function summary

Ran is a central part of the nucleocytoplasmic transport complex and directly participates in the regulation of genome organization and cell cycle progression. Ran's GTP-bound state helps maintain nuclear import and export balance supporting chromatin condensation and mitotic spindle assembly. This protein's complex involvement in the chromosomal region maintenance highlights its importance in cellular homeostasis emphasizing its role in organisms' growth and development.

Pathways

The mechanical functions of Ran impact the Ran GTPase pathway known for regulating nucleocytoplasmic transport and mitotic spindle organization. In the Ran cycle the protein works closely with RCC1 its guanine nucleotide exchange factor and RanGAP1 the GTPase-activating protein. These interactions are essential during cell division particularly for spindle assembly. Ran also aligns with pathways involving importins and exportins affecting protein export and nuclear import rates vital for cellular cyclic processes.

Ran has implications in cancer and neurodegenerative conditions. The protein's overexpression or dysfunction often correlate with various cancers due to its role in cell division and genetic material organization making it a focal point for cancer research. Additionally changes in Ran's regulatory pathways may influence neurodegenerative diseases by affecting nucleocytoplasmic transport in neurons. In these disorders Ran interacts with proteins such as CRM1 and others involved in the transport machinery highlighting its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs (PubMed : 10400640, PubMed : 17209048, PubMed : 26272610, PubMed : 27306458, PubMed : 8276887, PubMed : 8636225, PubMed : 8692944, PubMed : 8896452, PubMed : 9351834, PubMed : 9428644, PubMed : 9822603). Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis (PubMed : 11336674, PubMed : 26272610, PubMed : 29040603, PubMed : 7819259, PubMed : 8636225, PubMed : 8692944, PubMed : 8896452, PubMed : 9351834, PubMed : 9428644, PubMed : 9822603). Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN, while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensures the directionality of the transport (PubMed : 8896452, PubMed : 9351834, PubMed : 9428644). Interaction with RANBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins (PubMed : 20485264). RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation (PubMed : 10408446, PubMed : 29040603). Required for normal progress through mitosis (PubMed : 12194828, PubMed : 29040603, PubMed : 8421051). The complex with BIRC5/survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed : 18591255). Acts as a negative regulator of the kinase activity of VRK1 and VRK2 (PubMed : 18617507). Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases (PubMed : 10400640).

See full target information RAN

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biology direct 17:41 PubMed36528654

2022

LINC00858 stabilizes RAN expression and promotes metastasis of gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yunxin Lu,Qi Meng,Long Bai,Ruobing Wang,Yong Sun,Jiaqi Li,Jun Fan,Tian Tian

Immunity, inflammation and disease 10:e697 PubMed36169256

2022

Inhibiting P2Y12 receptor relieves LPS-induced inflammation and endothelial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuxia Han

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