AB209725

Anti-Ran antibody

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(1 Publication)

Rabbit Polyclonal RAN antibody. Suitable for WB, IHC-P and reacts with Zebrafish samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Zebrafish GTP-binding nuclear protein Ran aa 1 to C-terminus.

View Alternative Names

GTP-binding nuclear protein Ran, GTPase Ran, Ras-related nuclear protein, ran

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ran antibody (AB209725)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ran antibody (AB209725)

Immunohistochemical analysis of formalin fixed paraffin embedded Zebrafish tissue section labeling Ran with ab209725 at 1/300

Western blot - Anti-Ran antibody (AB209725)

WB

Supplier Data

Western blot - Anti-Ran antibody (AB209725)

12% SDS-PAGE

All lanes:

Western blot - Anti-Ran antibody (ab209725) at 1/1000 dilution

All lanes:

Zebrafish whole cell lysate at 30 µg

Predicted band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Zebrafish

Applications

WB, IHC-P

Immunogen

Recombinant Fragment Protein within Zebrafish GTP-binding nuclear protein Ran aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ran also known as Ras-related nuclear protein is a small GTP-binding protein with a mass of approximately 24 kDa. It localizes mainly in the nucleus and cytoplasm of cells and is highly expressed in actively dividing cells including various types of tumors. Ran plays a mechanical role in nucleocytoplasmic transport by facilitating the transport of proteins and ribonucleic acids through the nuclear pore complex. This protein belongs to the Ras superfamily of small GTPases which cycle between active GTP-bound and inactive GDP-bound states.

Biological function summary

Ran is a central part of the nucleocytoplasmic transport complex and directly participates in the regulation of genome organization and cell cycle progression. Ran's GTP-bound state helps maintain nuclear import and export balance supporting chromatin condensation and mitotic spindle assembly. This protein's complex involvement in the chromosomal region maintenance highlights its importance in cellular homeostasis emphasizing its role in organisms' growth and development.

Pathways

The mechanical functions of Ran impact the Ran GTPase pathway known for regulating nucleocytoplasmic transport and mitotic spindle organization. In the Ran cycle the protein works closely with RCC1 its guanine nucleotide exchange factor and RanGAP1 the GTPase-activating protein. These interactions are essential during cell division particularly for spindle assembly. Ran also aligns with pathways involving importins and exportins affecting protein export and nuclear import rates vital for cellular cyclic processes.

Ran has implications in cancer and neurodegenerative conditions. The protein's overexpression or dysfunction often correlate with various cancers due to its role in cell division and genetic material organization making it a focal point for cancer research. Additionally changes in Ran's regulatory pathways may influence neurodegenerative diseases by affecting nucleocytoplasmic transport in neurons. In these disorders Ran interacts with proteins such as CRM1 and others involved in the transport machinery highlighting its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs. Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis. Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN, while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensures the directionality of the transport. Interaction with RANBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins. RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation. Required for normal progress through mitosis.

See full target information GTP-binding nuclear protein Ran

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:23674 PubMed39389986

2024

Dietary Chlorella vulgaris supplementation modulates health, microbiota and the response to oxidative stress of Atlantic salmon.

Applications

Unspecified application

Species

Unspecified reactive species

Jonas Mueller,Doret R van Muilekom,Jannick Ehlers,Marvin Suhr,Stéphanie C Hornburg,Corinna Bang,Marie Wilkes,Thekla Schultheiß,Edmund Maser,Alexander Rebl,Tom Goldammer,Henrike Seibel,Carsten Schulz

View all publications

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Product promise

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