AB53775

Anti-Ran antibody

5

(2 Reviews)

|

(10 Publications)

Rabbit Polyclonal RAN antibody. Suitable for ELISA, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human RAN.

View Alternative Names

ARA24, OK/SW-cl.81, RAN, GTP-binding nuclear protein Ran, Androgen receptor-associated protein 24, GTPase Ran, Ras-like protein TC4, Ras-related nuclear protein

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ran antibody (AB53775)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ran antibody (AB53775)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab53775 at a 1/50 dilution.

Left image un-treated.

Right image treated with immunizing peptide.

Western blot - Anti-Ran antibody (AB53775)

WB

Unknown

Western blot - Anti-Ran antibody (AB53775)

All lanes:

Western blot - Anti-Ran antibody (ab53775) at 1/500 dilution

Lane 1:

Extracts from LOVO cells

Lane 2:

Extracts from LOVO cells, plus immunising peptide

Predicted band size: 24 kDa

Observed band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Rat, Mouse

Applications

ICC/IF, WB, IHC-P, ELISA

Immunogen

Synthetic Peptide within Human RAN. The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Alternative Products

Primary Antibodies

AB155103

Anti-Ran antibody [EPR10791(B)]

RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-Ran antibody [EPR10791(B)] (AB155103)

0
0

Primary Antibodies

AB209725

Anti-Ran antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ran antibody (AB209725)

0
0

Primary Antibodies

AB233762

Anti-Ran antibody [8D1H12]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ran antibody [8D1H12] (AB233762)

0
0

Primary Antibodies

AB226195

Anti-Ran antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ran antibody (AB226195)

0
0

Primary Antibodies

AB157213

Anti-Ran antibody [EPR10790(B)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Ran antibody [EPR10790(B)] (AB157213)

0
0

Primary Antibodies

AB31118

Anti-Ran antibody

0
0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

5
20

Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

5
88

Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

5
104

Primary Antibodies

AB133323

Anti-14-3-3 antibody [EPR6379]

RabMAb|Recombinant

Western blot - Anti-14-3-3 antibody [EPR6379] (AB133323)

0
0

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ran also known as Ras-related nuclear protein is a small GTP-binding protein with a mass of approximately 24 kDa. It localizes mainly in the nucleus and cytoplasm of cells and is highly expressed in actively dividing cells including various types of tumors. Ran plays a mechanical role in nucleocytoplasmic transport by facilitating the transport of proteins and ribonucleic acids through the nuclear pore complex. This protein belongs to the Ras superfamily of small GTPases which cycle between active GTP-bound and inactive GDP-bound states.

Biological function summary

Ran is a central part of the nucleocytoplasmic transport complex and directly participates in the regulation of genome organization and cell cycle progression. Ran's GTP-bound state helps maintain nuclear import and export balance supporting chromatin condensation and mitotic spindle assembly. This protein's complex involvement in the chromosomal region maintenance highlights its importance in cellular homeostasis emphasizing its role in organisms' growth and development.

Pathways

The mechanical functions of Ran impact the Ran GTPase pathway known for regulating nucleocytoplasmic transport and mitotic spindle organization. In the Ran cycle the protein works closely with RCC1 its guanine nucleotide exchange factor and RanGAP1 the GTPase-activating protein. These interactions are essential during cell division particularly for spindle assembly. Ran also aligns with pathways involving importins and exportins affecting protein export and nuclear import rates vital for cellular cyclic processes.

Ran has implications in cancer and neurodegenerative conditions. The protein's overexpression or dysfunction often correlate with various cancers due to its role in cell division and genetic material organization making it a focal point for cancer research. Additionally changes in Ran's regulatory pathways may influence neurodegenerative diseases by affecting nucleocytoplasmic transport in neurons. In these disorders Ran interacts with proteins such as CRM1 and others involved in the transport machinery highlighting its potential as a therapeutic target.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs (PubMed : 10400640, PubMed : 17209048, PubMed : 26272610, PubMed : 27306458, PubMed : 8276887, PubMed : 8636225, PubMed : 8692944, PubMed : 8896452, PubMed : 9351834, PubMed : 9428644, PubMed : 9822603). Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis (PubMed : 11336674, PubMed : 26272610, PubMed : 29040603, PubMed : 7819259, PubMed : 8636225, PubMed : 8692944, PubMed : 8896452, PubMed : 9351834, PubMed : 9428644, PubMed : 9822603). Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN, while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensures the directionality of the transport (PubMed : 8896452, PubMed : 9351834, PubMed : 9428644). Interaction with RANBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins (PubMed : 20485264). RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation (PubMed : 10408446, PubMed : 29040603). Required for normal progress through mitosis (PubMed : 12194828, PubMed : 29040603, PubMed : 8421051). The complex with BIRC5/survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed : 18591255). Acts as a negative regulator of the kinase activity of VRK1 and VRK2 (PubMed : 18617507). Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases (PubMed : 10400640).

See full target information RAN

style

left-column

Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceuticals (Basel, Switzerland) 17: PubMed39770503

2025

The Small GTPase Ran Increases Sensitivity of Ovarian Cancer Cells to Oncolytic Vesicular Stomatitis Virus.

Applications

Unspecified application

Species

Unspecified reactive species

Karen Geoffroy,Mélissa Viens,Emma Mary Kalin,Zied Boudhraa,Dominic Guy Roy,Jian Hui Wu,Diane Provencher,Anne-Marie Mes-Masson,Marie-Claude Bourgeois-Daigneault

The Journal of cell biology 220: PubMed33909044

2021

O-GlcNAc modification of nuclear pore complexes accelerates bidirectional transport.

Applications

Unspecified application

Species

Unspecified reactive species

Tae Yeon Yoo,Timothy J Mitchison

International journal of biological sciences 16:1941-1953 PubMed32398961

2020

Lin28A Regulates Stem-like Properties of Ovarian Cancer Cells by Enriching RAN and HSBP1 mRNA and Up-regulating its Protein Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yancheng Zhong,Lanqin Cao,Haotian Ma,Qian Wang,Pingpin Wei,Juan Yang,Yuqing Mo,Lihua Cao,Cijun Shuai,Shuping Peng

Cancer management and research 12:2291-2300 PubMed32273766

2020

MiR-802 Suppresses Colorectal Cancer Cell Viability, Migration and Invasion by Targeting RAN.

Applications

Unspecified application

Species

Unspecified reactive species

Hailong Feng,Lingling Liu,Laijing Xu,Haili Wang,Qiuju Hua,Peng He

eLife 6: PubMed28994651

2017

acts in the PLK1-dependent spindle positioning pathway and supports neural development.

Applications

Unspecified application

Species

Unspecified reactive species

Marisa Connell,Helen Chen,Jihong Jiang,Chia-Wei Kuan,Abbas Fotovati,Tony Lh Chu,Zhengcheng He,Tess C Lengyell,Huaibiao Li,Torsten Kroll,Amanda M Li,Daniel Goldowitz,Lucien Frappart,Aspasia Ploubidou,Millan S Patel,Linda M Pilarski,Elizabeth M Simpson,Philipp F Lange,Douglas W Allan,Christopher A Maxwell

Human molecular genetics 26:790-800 PubMed28040728

2017

Cytoplasmic poly-GA aggregates impair nuclear import of TDP-43 in C9orf72 ALS/FTLD.

Applications

Unspecified application

Species

Unspecified reactive species

Bahram Khosravi,Hannelore Hartmann,Stephanie May,Christoph Möhl,Helena Ederle,Meike Michaelsen,Martin H Schludi,Dorothee Dormann,Dieter Edbauer

PloS one 8:e53349 PubMed23308199

2013

Reduced RAN expression and disrupted transport between cytoplasm and nucleus; a key event in Alzheimer's disease pathophysiology.

Applications

IHC-P, WB, ICC/IF

Species

Human, Human, Human

Diego Mastroeni,Leonidas Chouliaras,Andrew Grover,Winnie S Liang,Kevin Hauns,Joseph Rogers,Paul D Coleman

Diabetes 62:1738-45 PubMed23274905

2013

Genetic and molecular insights into the role of PROX1 in glucose metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Sophie Lecompte,Gianni Pasquetti,Xavier Hermant,Benjamin Grenier-Boley,Marcela Gonzalez-Gross,Stephan De Henauw,Denes Molnar,Peter Stehle,Laurent Béghin,Luis A Moreno,Philippe Amouyel,Jean Dallongeville,Aline Meirhaeghe

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 25:948-59 PubMed21106934

2010

A mitochondrial enzyme degrades carotenoids and protects against oxidative stress.

Applications

WB

Species

Mouse

Jaume Amengual,Glenn P Lobo,Marcin Golczak,Hua Nan M Li,Tatyana Klimova,Charles L Hoppel,Adrian Wyss,Krzysztof Palczewski,Johannes von Lintig

The Journal of biological chemistry 285:27891-9 PubMed20573961

2010

Beta,beta-carotene decreases peroxisome proliferator receptor gamma activity and reduces lipid storage capacity of adipocytes in a beta,beta-carotene oxygenase 1-dependent manner.

Applications

WB

Species

Mouse

Glenn P Lobo,Jaume Amengual,Hua Nan M Li,Marcin Golczak,M Luisa Bonet,Krzysztof Palczewski,Johannes von Lintig

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com