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AB249315

Anti-Ran antibody [EPR10790(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal RAN antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.

View Alternative Names

ARA24, OK/SW-cl.81, RAN, GTP-binding nuclear protein Ran, Androgen receptor-associated protein 24, GTPase Ran, Ras-like protein TC4, Ras-related nuclear protein

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Ran antibody [EPR10790(B)] - BSA and Azide free (AB249315)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Ran antibody [EPR10790(B)] - BSA and Azide free (AB249315)

This data was developed using ab157213, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 293T cells, labeling Ran with ab157213 at 1/100 dilution.

Western blot - Anti-Ran antibody [EPR10790(B)] - BSA and Azide free (AB249315)
  • WB

Lab

Western blot - Anti-Ran antibody [EPR10790(B)] - BSA and Azide free (AB249315)

This data was developed using ab157213, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The bands observed at 30kDa, 60kDa and 150kDa should be monomer, dimer and polymer respectively.

Lanes 1 - 2:

Western blot - Anti-Ran antibody [EPR10790(B)] (<a href='/en-us/products/primary-antibodies/ran-antibody-epr10790b-ab157213'>ab157213</a>) at 1/1000 dilution

Lanes 1 - 2:

Western blot - Anti-Ran antibody [EPR10790(B)] - BSA and Azide free (ab249315) at 1/1000 dilution

Lane 1:

HEK-293T transfected with an empty vector whole cell lysate at 20 µg

Lane 2:

HEK-293T transfected with a human Ran expression vector containing a Flag tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 24 kDa,30 kDa,60 kDa,150 kDa

false

Exposure time: 7s

Western blot - Anti-Ran antibody [EPR10790(B)] - BSA and Azide free (AB249315)
  • WB

Unknown

Western blot - Anti-Ran antibody [EPR10790(B)] - BSA and Azide free (AB249315)

This data was developed using ab157213, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Ran antibody [EPR10790(B)] (<a href='/en-us/products/primary-antibodies/ran-antibody-epr10790b-ab157213'>ab157213</a>) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Predicted band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10790(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab249315 is the carrier-free version of ab157213.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ran also known as Ras-related nuclear protein is a small GTP-binding protein with a mass of approximately 24 kDa. It localizes mainly in the nucleus and cytoplasm of cells and is highly expressed in actively dividing cells including various types of tumors. Ran plays a mechanical role in nucleocytoplasmic transport by facilitating the transport of proteins and ribonucleic acids through the nuclear pore complex. This protein belongs to the Ras superfamily of small GTPases which cycle between active GTP-bound and inactive GDP-bound states.
Biological function summary

Ran is a central part of the nucleocytoplasmic transport complex and directly participates in the regulation of genome organization and cell cycle progression. Ran's GTP-bound state helps maintain nuclear import and export balance supporting chromatin condensation and mitotic spindle assembly. This protein's complex involvement in the chromosomal region maintenance highlights its importance in cellular homeostasis emphasizing its role in organisms' growth and development.

Pathways

The mechanical functions of Ran impact the Ran GTPase pathway known for regulating nucleocytoplasmic transport and mitotic spindle organization. In the Ran cycle the protein works closely with RCC1 its guanine nucleotide exchange factor and RanGAP1 the GTPase-activating protein. These interactions are essential during cell division particularly for spindle assembly. Ran also aligns with pathways involving importins and exportins affecting protein export and nuclear import rates vital for cellular cyclic processes.

Ran has implications in cancer and neurodegenerative conditions. The protein's overexpression or dysfunction often correlate with various cancers due to its role in cell division and genetic material organization making it a focal point for cancer research. Additionally changes in Ran's regulatory pathways may influence neurodegenerative diseases by affecting nucleocytoplasmic transport in neurons. In these disorders Ran interacts with proteins such as CRM1 and others involved in the transport machinery highlighting its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs (PubMed : 10400640, PubMed : 17209048, PubMed : 26272610, PubMed : 27306458, PubMed : 8276887, PubMed : 8636225, PubMed : 8692944, PubMed : 8896452, PubMed : 9351834, PubMed : 9428644, PubMed : 9822603). Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis (PubMed : 11336674, PubMed : 26272610, PubMed : 29040603, PubMed : 7819259, PubMed : 8636225, PubMed : 8692944, PubMed : 8896452, PubMed : 9351834, PubMed : 9428644, PubMed : 9822603). Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN, while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensures the directionality of the transport (PubMed : 8896452, PubMed : 9351834, PubMed : 9428644). Interaction with RANBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins (PubMed : 20485264). RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation (PubMed : 10408446, PubMed : 29040603). Required for normal progress through mitosis (PubMed : 12194828, PubMed : 29040603, PubMed : 8421051). The complex with BIRC5/survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed : 18591255). Acts as a negative regulator of the kinase activity of VRK1 and VRK2 (PubMed : 18617507). Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases (PubMed : 10400640).
See full target information RAN
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Product promise

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