AB2937

Anti-RanBP1 antibody

Name: Anti-RanBP1 antibody (ab2937) | Abcam
Brand: Abcam Limited
SKU: AB2937
Price: 525 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal RanBP1 antibody. Suitable for ICC/IF, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human RANBP1 aa 1-50.

View Alternative Names

Ran-specific GTPase-activating protein, Ran-binding protein 1, RanBP1, RANBP1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-RanBP1 antibody (AB2937)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RanBP1 antibody (AB2937)

Immunocytochemistry analysis of HeLa cells labeling RanB1 with ab2937 at 2 μg/mL. Cells were fixed in 4% Paraformaldehyde, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 1% BSA for 1 hour at RT. Cells were incubated for 3 hours at RT and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor^® 488 conjugate at 1 : 2000 dilution for 45 minutes at RT (Panel A : green). Nuclei (Panel B : blue) were stained with DAPI. F-actin (Panel C : red) was stained with Alexa Fluor^® 555 Rhodamine Phalloidin at 1 : 300 dilution. Panel D is a merged image showing cytoplasmic localization. Panel E shows no primary antibody control. The images were captured at 60X magnification.

Supplier Data

Western blot - Anti-RanBP1 antibody (AB2937)

All lanes:

Western blot - Anti-RanBP1 antibody (ab2937) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 25 µg

Lane 2:

Human brain tissue lysate at 25 µg

Lane 3:

NIH-3T3 cell lysate at 25 µg

Secondary

All lanes:

HRP conjugated anti-rabbit

Predicted band size: 23 kDa

Observed band size: 27 kDa

true

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human RANBP1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P43487

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2 µg/mL", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RanBP1 also known as Ran-binding protein 1 is an important player in the regulation of the Ran GTPase cycle integral to transport processes across the nuclear envelope. This protein has a mass of about 23 kDa. In humans RanBP1 shows expression in various tissues with higher levels observed in testis and brain. It directly interacts with Ran a small GTPase helping to modulate its activity by promoting the conversion between the active GTP-bound form and the inactive GDP-bound form.

Biological function summary

RanBP1 functions closely with Ran GTPase in the nucleocytoplasmic transport system where it is part of a complex with Ran and other factors like importins and exportins. It aids in the stabilization of the Ran-GTPase complex facilitating the release of cargo molecules and ensuring efficient transport cycles within the cells. RanBP1 in conjunction with Ran regulates the dynamic transport of proteins and RNA essential for maintaining cellular compartmentalization.

Pathways

The RanBP1 protein plays a role in the Ran GTPase cycle and nucleocytoplasmic transport pathways. These pathways are essential to cellular dynamics affecting overall transport between the cytoplasm and the nucleus. Furthermore RanBP1 affects interactions with proteins such as RanGAP1 contributing to the regulation of Ran activity cycles and supporting cellular functions including mitosis and RNA processing.

Research indicates that alterations in RanBP1 expression and function relate to cancer progression and neurodegenerative conditions. Misregulation of RanBP1 can affect nuclear transport balance impacting cancer-promoting pathways. Its interaction with Ran and its pathway-related proteins like importin beta suggests a role in tumorigenesis by contributing to aberrant cell cycle regulation and mitotic errors while its importance in neuron function suggests involvement in neurodegenerative disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in RAN-dependent nucleocytoplasmic transport. Alleviates the TNPO1-dependent inhibition of RAN GTPase activity and mediates the dissociation of RAN from proteins involved in transport into the nucleus (By similarity). Induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins (PubMed : 20485264). Promotes the disassembly of the complex formed by RAN and importin beta. Promotes dissociation of RAN from a complex with KPNA2 and CSE1L (By similarity). Required for normal mitotic spindle assembly and normal progress through mitosis via its effect on RAN (PubMed : 17671426). Does not increase the RAN GTPase activity by itself, but increases GTP hydrolysis mediated by RANGAP1 (PubMed : 7882974). Inhibits RCC1-dependent exchange of RAN-bound GDP by GTP (PubMed : 7616957, PubMed : 7882974).

See full target information RANBP1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular & cellular proteomics : MCP 24:100936 PubMed40044042

2025

Upregulation of Protein O-GlcNAcylation Levels Promotes Zebrafish Fin Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Liyuan Jia,Hanxue Zheng,Juantao Feng,Yi Ding,Xiaotian Sun,Yuan Yu,Xue Hao,Junxiang Wang,Xinyu Zhang,Yuanfeng Tian,Fulin Chen,Jihong Cui

Scientific reports 6:33996 PubMed27665936

2016

TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins.

Applications

WB, ICC/IF

Species

Unspecified reactive species, Unspecified reactive species

S Prpar Mihevc,Marco Baralle,Emanuele Buratti,Boris Rogelj

Neuroscience 293:157-70 PubMed25743254

2015

Proteomic analyses reveal that loss of TDP-43 affects RNA processing and intracellular transport.

Applications

ICC/IF, WB

Species

Unspecified reactive species, Unspecified reactive species

M Štalekar,X Yin,K Rebolj,S Darovic,C Troakes,M Mayr,C E Shaw,B Rogelj

Journal of proteome research 6:3752-9 PubMed17705417

2007

Proteomic analysis of peripheral blood mononuclear cells: selective protein processing observed in patients with rheumatoid arthritis.

Applications

Species

Human

Martin Schulz,Helmut Dotzlaw,Stefan Mikkat,Martin Eggert,Gunther Neeck

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-RanBP1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RanBP1 antibody (AB2937)

Western blot - Anti-RanBP1 antibody (AB2937)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise