Anti-RanBP1 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RanBP1 antibody (AB97659)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling Ran BP1 protein at cytoplasm using ab97659 at a 1/500 dilution (Green).
alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at a 1/10000 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue).

• WB

Unknown

Western blot - Anti-RanBP1 antibody (AB97659)

12% SDS-PAGE.

All lanes:

Western blot - Anti-RanBP1 antibody (ab97659) at 1/1000 dilution

All lanes:

HeLa S3 whole cell lysate at 30 µg

Predicted band size: 23 kDa

false

• WB

Supplier Data

Western blot - Anti-RanBP1 antibody (AB97659)

Samples were separated by 12% SDS PAGE.

All lanes:

Western blot - Anti-RanBP1 antibody (ab97659) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 23 kDa

false

• WB

Supplier Data

Western blot - Anti-RanBP1 antibody (AB97659)

Samples were separated by 12% SDS PAGE.

All lanes:

Western blot - Anti-RanBP1 antibody (ab97659) at 1/1000 dilution

All lanes:

Mouse brain tissue lysate at 50 µg

Predicted band size: 24 kDa

false

• WB

Supplier Data

Western blot - Anti-RanBP1 antibody (AB97659)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-RanBP1 antibody (ab97659) at 1/2000 dilution

Lane 1:

Wild-type (WT) 293T cell extracts at 30 µg

Lane 2:

RanBP1 knockout (KO) 293T cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 23 kDa

false