AB133550

Anti-RanBP1 antibody [EPR6796(B)]

RabMAb
Recombinant
KO Validated
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Rabbit Recombinant Monoclonal RanBP1 antibody. Suitable for WB and reacts with Human samples.

View Alternative Names

Ran-specific GTPase-activating protein, Ran-binding protein 1, RanBP1, RANBP1

2 Images

Lab

Western blot - Anti-RanBP1 antibody [EPR6796(B)] (AB133550)

Lanes 1-4 : Merged signal (red and green). Green - ab133550 observed at 30 kDa. Red - loading control ab8245 observed at 36 kDa.

ab133550 Anti-RanBP1 antibody [EPR6796(B)] was shown to specifically react with RanBP1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266202 (knockout cell lysate ab258168) was used. Wild-type and RanBP1 knockout samples were subjected to SDS-PAGE. ab133550 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-RanBP1 antibody [EPR6796(B)] (ab133550) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

RANBP1 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human RANBP1 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-ranbp1-knockout-hek-293t-cell-line-ab266202'>ab266202</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 23 kDa,54 kDa,78 kDa

Observed band size: 30 kDa,70-95 kDa,78 kDa

false

Unknown

Western blot - Anti-RanBP1 antibody [EPR6796(B)] (AB133550)

All lanes:

Western blot - Anti-RanBP1 antibody [EPR6796(B)] (ab133550) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 23 kDa

false

Carrier free

Anti-RanBP1 antibody [EPR6796(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6796(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RanBP1 also known as Ran-binding protein 1 is an important player in the regulation of the Ran GTPase cycle integral to transport processes across the nuclear envelope. This protein has a mass of about 23 kDa. In humans RanBP1 shows expression in various tissues with higher levels observed in testis and brain. It directly interacts with Ran a small GTPase helping to modulate its activity by promoting the conversion between the active GTP-bound form and the inactive GDP-bound form.

Biological function summary

RanBP1 functions closely with Ran GTPase in the nucleocytoplasmic transport system where it is part of a complex with Ran and other factors like importins and exportins. It aids in the stabilization of the Ran-GTPase complex facilitating the release of cargo molecules and ensuring efficient transport cycles within the cells. RanBP1 in conjunction with Ran regulates the dynamic transport of proteins and RNA essential for maintaining cellular compartmentalization.

Pathways

The RanBP1 protein plays a role in the Ran GTPase cycle and nucleocytoplasmic transport pathways. These pathways are essential to cellular dynamics affecting overall transport between the cytoplasm and the nucleus. Furthermore RanBP1 affects interactions with proteins such as RanGAP1 contributing to the regulation of Ran activity cycles and supporting cellular functions including mitosis and RNA processing.

Research indicates that alterations in RanBP1 expression and function relate to cancer progression and neurodegenerative conditions. Misregulation of RanBP1 can affect nuclear transport balance impacting cancer-promoting pathways. Its interaction with Ran and its pathway-related proteins like importin beta suggests a role in tumorigenesis by contributing to aberrant cell cycle regulation and mitotic errors while its importance in neuron function suggests involvement in neurodegenerative disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in RAN-dependent nucleocytoplasmic transport. Alleviates the TNPO1-dependent inhibition of RAN GTPase activity and mediates the dissociation of RAN from proteins involved in transport into the nucleus (By similarity). Induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins (PubMed : 20485264). Promotes the disassembly of the complex formed by RAN and importin beta. Promotes dissociation of RAN from a complex with KPNA2 and CSE1L (By similarity). Required for normal mitotic spindle assembly and normal progress through mitosis via its effect on RAN (PubMed : 17671426). Does not increase the RAN GTPase activity by itself, but increases GTP hydrolysis mediated by RANGAP1 (PubMed : 7882974). Inhibits RCC1-dependent exchange of RAN-bound GDP by GTP (PubMed : 7616957, PubMed : 7882974).

See full target information RANBP1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RanBP1 antibody [EPR6796(B)]

Western blot - Anti-RanBP1 antibody [EPR6796(B)] (AB133550)

Western blot - Anti-RanBP1 antibody [EPR6796(B)] (AB133550)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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