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Rabbit Recombinant Monoclonal RanBP1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-RanBP1 antibody [EPR6796(B)] - BSA and Azide free (AB248549), expandable thumbnail
  • Western blot - Anti-RanBP1 antibody [EPR6796(B)] - BSA and Azide free (AB248549), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-P
Human
Not recommended
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Plays a role in RAN-dependent nucleocytoplasmic transport. Alleviates the TNPO1-dependent inhibition of RAN GTPase activity and mediates the dissociation of RAN from proteins involved in transport into the nucleus (By similarity). Induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins (PubMed:20485264). Promotes the disassembly of the complex formed by RAN and importin beta. Promotes dissociation of RAN from a complex with KPNA2 and CSE1L (By similarity). Required for normal mitotic spindle assembly and normal progress through mitosis via its effect on RAN (PubMed:17671426). Does not increase the RAN GTPase activity by itself, but increases GTP hydrolysis mediated by RANGAP1 (PubMed:7882974). Inhibits RCC1-dependent exchange of RAN-bound GDP by GTP (PubMed:7616957, PubMed:7882974).

Alternative names

Ran-specific GTPase-activating protein, Ran-binding protein 1, RanBP1, RANBP1

Recommended products

Rabbit Recombinant Monoclonal RanBP1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR6796(B)
Purification technique
Affinity purification
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248549 is the carrier-free version of Anti-RanBP1 antibody [EPR6796(B)] ab133550.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RanBP1 also known as Ran-binding protein 1 is an important player in the regulation of the Ran GTPase cycle integral to transport processes across the nuclear envelope. This protein has a mass of about 23 kDa. In humans RanBP1 shows expression in various tissues with higher levels observed in testis and brain. It directly interacts with Ran a small GTPase helping to modulate its activity by promoting the conversion between the active GTP-bound form and the inactive GDP-bound form.

Biological function summary

RanBP1 functions closely with Ran GTPase in the nucleocytoplasmic transport system where it is part of a complex with Ran and other factors like importins and exportins. It aids in the stabilization of the Ran-GTPase complex facilitating the release of cargo molecules and ensuring efficient transport cycles within the cells. RanBP1 in conjunction with Ran regulates the dynamic transport of proteins and RNA essential for maintaining cellular compartmentalization.

Pathways

The RanBP1 protein plays a role in the Ran GTPase cycle and nucleocytoplasmic transport pathways. These pathways are essential to cellular dynamics affecting overall transport between the cytoplasm and the nucleus. Furthermore RanBP1 affects interactions with proteins such as RanGAP1 contributing to the regulation of Ran activity cycles and supporting cellular functions including mitosis and RNA processing.

Associated diseases and disorders

Research indicates that alterations in RanBP1 expression and function relate to cancer progression and neurodegenerative conditions. Misregulation of RanBP1 can affect nuclear transport balance impacting cancer-promoting pathways. Its interaction with Ran and its pathway-related proteins like importin beta suggests a role in tumorigenesis by contributing to aberrant cell cycle regulation and mitotic errors while its importance in neuron function suggests involvement in neurodegenerative disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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