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AB317209

Anti-RanBP1 antibody [HL2358]

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Rabbit Monoclonal RanBP1 antibody. Suitable for ICC/IF, WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human RANBP1.

View Alternative Names

Ran-specific GTPase-activating protein, Ran-binding protein 1, RanBP1, RANBP1

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-RanBP1 antibody [HL2358] (AB317209)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RanBP1 antibody [HL2358] (AB317209)

ab317209 detects RanBP1 protein at cytoplasm by immunofluorescent analysis. Sample : HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green : RanBP1 stained by ab317209 diluted at 1 : 500. Blue : Fluoroshield with DAPI.

Western blot - Anti-RanBP1 antibody [HL2358] (AB317209)
  • WB

Supplier Data

Western blot - Anti-RanBP1 antibody [HL2358] (AB317209)

Wild-type (WT) and RanBP1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE and the membrane was blotted with ab317209 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-RanBP1 antibody [HL2358] (ab317209) at 1/1000 dilution

Lane 1:

Wild-type (WT) 293T cell extracts at 30 µg

Lane 2:

RanBP1 knockout (KO) 293T cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-RanBP1 antibody [HL2358] (AB317209)
  • WB

Supplier Data

Western blot - Anti-RanBP1 antibody [HL2358] (AB317209)

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE and the membrane was blotted with ab317209 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-RanBP1 antibody [HL2358] (ab317209) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-RanBP1 antibody [HL2358] (AB317209)
  • WB

Supplier Data

Western blot - Anti-RanBP1 antibody [HL2358] (AB317209)

Various tissue extracts (50 ug) were separated by 12% SDS-PAGE and the membrane was blotted with ab317209 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-RanBP1 antibody [HL2358] (ab317209) at 1/1000 dilution

Lane 1:

Mouse testis tissue extracts at 50 µg

Lane 2:

Rat testis tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2358

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human RANBP1.

P43487

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RanBP1 also known as Ran-binding protein 1 is an important player in the regulation of the Ran GTPase cycle integral to transport processes across the nuclear envelope. This protein has a mass of about 23 kDa. In humans RanBP1 shows expression in various tissues with higher levels observed in testis and brain. It directly interacts with Ran a small GTPase helping to modulate its activity by promoting the conversion between the active GTP-bound form and the inactive GDP-bound form.
Biological function summary

RanBP1 functions closely with Ran GTPase in the nucleocytoplasmic transport system where it is part of a complex with Ran and other factors like importins and exportins. It aids in the stabilization of the Ran-GTPase complex facilitating the release of cargo molecules and ensuring efficient transport cycles within the cells. RanBP1 in conjunction with Ran regulates the dynamic transport of proteins and RNA essential for maintaining cellular compartmentalization.

Pathways

The RanBP1 protein plays a role in the Ran GTPase cycle and nucleocytoplasmic transport pathways. These pathways are essential to cellular dynamics affecting overall transport between the cytoplasm and the nucleus. Furthermore RanBP1 affects interactions with proteins such as RanGAP1 contributing to the regulation of Ran activity cycles and supporting cellular functions including mitosis and RNA processing.

Research indicates that alterations in RanBP1 expression and function relate to cancer progression and neurodegenerative conditions. Misregulation of RanBP1 can affect nuclear transport balance impacting cancer-promoting pathways. Its interaction with Ran and its pathway-related proteins like importin beta suggests a role in tumorigenesis by contributing to aberrant cell cycle regulation and mitotic errors while its importance in neuron function suggests involvement in neurodegenerative disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in RAN-dependent nucleocytoplasmic transport. Alleviates the TNPO1-dependent inhibition of RAN GTPase activity and mediates the dissociation of RAN from proteins involved in transport into the nucleus (By similarity). Induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins (PubMed : 20485264). Promotes the disassembly of the complex formed by RAN and importin beta. Promotes dissociation of RAN from a complex with KPNA2 and CSE1L (By similarity). Required for normal mitotic spindle assembly and normal progress through mitosis via its effect on RAN (PubMed : 17671426). Does not increase the RAN GTPase activity by itself, but increases GTP hydrolysis mediated by RANGAP1 (PubMed : 7882974). Inhibits RCC1-dependent exchange of RAN-bound GDP by GTP (PubMed : 7616957, PubMed : 7882974).
See full target information RANBP1
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Product promise

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