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AB245563

Anti-RanBP2 antibody

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(1 Publication)

Rabbit Polyclonal RanBP2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RANBP2 aa 2600-2650.

View Alternative Names

NUP358, RANBP2, E3 SUMO-protein ligase RanBP2, 358 kDa nucleoporin, Nuclear pore complex protein Nup358, Nucleoporin Nup358, Ran-binding protein 2, p270, RanBP2

2 Images
Immunoprecipitation - Anti-RanBP2 antibody (AB245563)
  • IP

Supplier Data

Immunoprecipitation - Anti-RanBP2 antibody (AB245563)

RanBP2 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).

ab245563 used for IP at 3 μg/mg lysate. For WB 0.1 μg/ml.

Lane 1 : ab245563 IP in HeLa whole cell lysate.
Lane 2 : Control IgG in HeLa whole cell lysate.
Chemiluminescence detection : 1 second.

All lanes:

Immunoprecipitation - Anti-RanBP2 antibody (ab245563)

Predicted band size: 358 kDa

false

Western blot - Anti-RanBP2 antibody (AB245563)
  • WB

Supplier Data

Western blot - Anti-RanBP2 antibody (AB245563)

All lanes:

Western blot - Anti-RanBP2 antibody (ab245563) at 0.04 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 358 kDa

false

Exposure time: 5s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human RANBP2 aa 2600-2650. The exact immunogen used to generate this antibody is proprietary information.

P49792

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab245563 was affinity purified using an epitope specific to RanBP2 immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RanBP2 also known as Nucleoporin 358 (Nup358) is a large protein with a molecular mass of approximately 358 kDa. It is an important component of the nuclear pore complex (NPC) and is found on the cytoplasmic side of the nuclear envelope. RanBP2 is expressed in a variety of tissues with higher levels in the brain and testis. Its alternates names include Nup358 due to its role as a nucleoporin. Mechanically RanBP2 facilitates nucleocytoplasmic transport by interacting with the small GTPase Ran.
Biological function summary

RanBP2 plays a significant role in multiple processes such as mitotic spindle assembly and chromatin condensation. It functions as part of the Ran GTPase system a critical complex for nucleocytoplasmic transport. In addition RanBP2 contributes to protein sumoylation where it acts as a scaffold for conjugating enzymes. It maintains nuclear envelope integrity and supports the positioning of centrosomes during cell division.

Pathways

RanBP2 is actively involved in the nucleocytoplasmic transport pathway ensuring the proper exchange of proteins and RNA between the nucleus and cytoplasm. It is critical to the Ran cycle where it works alongside proteins like RanGTP and RanGAP1 to regulate transport activities. The involvement of RanBP2 in the SUMOylation pathway also emphasizes its role in post-translational modification impacting various cellular events and signaling.

Changes in RanBP2 expression have been linked to neurodegenerative diseases and certain types of cancer. In neurodegenerative conditions such as hereditary spastic paraplegia RanBP2 forms a complex with proteins like atlastin-1 which further influences disease progression. In cancer RanBP2 interacts with oncogenic pathways through its role in cell cycle regulation highlighting its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I (PubMed : 11792325, PubMed : 12032081, PubMed : 15378033, PubMed : 15931224, PubMed : 22194619). Involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates (PubMed : 7775481). Binds single-stranded RNA (in vitro) (PubMed : 7775481). May bind DNA (PubMed : 7775481). Component of the nuclear export pathway (PubMed : 10078529). Specific docking site for the nuclear export factor exportin-1 (PubMed : 10078529). Inhibits EIF4E-dependent mRNA export (PubMed : 22902403). Sumoylates PML at 'Lys-490' which is essential for the proper assembly of PML-NB (PubMed : 22155184). Recruits BICD2 to the nuclear envelope and cytoplasmic stacks of nuclear pore complex known as annulate lamellae during G2 phase of cell cycle (PubMed : 20386726). Probable inactive PPIase with no peptidyl-prolyl cis-trans isomerase activity (PubMed : 20676357, PubMed : 23353830).
See full target information RANBP2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 94: PubMed31694957

2020

Foot-and-Mouth Disease Virus Capsid Protein VP1 Interacts with Host Ribosomal Protein SA To Maintain Activation of the MAPK Signal Pathway and Promote Virus Replication.

Applications

Unspecified application

Species

Unspecified reactive species

Zixiang Zhu,Weiwei Li,Xiangle Zhang,Congcong Wang,Lili Gao,Fan Yang,Weijun Cao,Kangli Li,Hong Tian,Xiangtao Liu,Keshan Zhang,Haixue Zheng
View all publications
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Product promise

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