AB2938

Anti-RanBP2 antibody

Name: Anti-RanBP2 antibody (ab2938) | Abcam
Brand: Abcam Limited
SKU: AB2938
Price: 545 USD
Availability: InStock

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(9 Publications)

Rabbit Polyclonal RanBP2 antibody. Suitable for WB, IHC-P and reacts with Human, Cow samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human RANBP2 aa 1-50.

View Alternative Names

NUP358, RANBP2, E3 SUMO-protein ligase RanBP2, 358 kDa nucleoporin, Nuclear pore complex protein Nup358, Nucleoporin Nup358, Ran-binding protein 2, p270, RanBP2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP2 antibody (AB2938)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP2 antibody (AB2938)

Immunohistochemical staining of RanBP2 in bovine retina using ab2938.

Unknown

Western blot - Anti-RanBP2 antibody (AB2938)

Western blot of RanBP2 from human retinal extracts using ab2938.

All lanes:

Western blot - Anti-RanBP2 antibody (ab2938)

Predicted band size: 358 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Cow, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human RANBP2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P49792

Specificity

Detects Ran binding protein 2 (RanBP 2) from human and bovine retina.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p>Detects a band of approximately 360 kDa representing RanBP 2 from bovine retinal extracts.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p>Immunohistochemical staining of bovine retina results in cytoplasmic staining of cells within the inner nuclear layer as well as the ganglion cells.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: 99% PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RanBP2 also known as Nucleoporin 358 (Nup358) is a large protein with a molecular mass of approximately 358 kDa. It is an important component of the nuclear pore complex (NPC) and is found on the cytoplasmic side of the nuclear envelope. RanBP2 is expressed in a variety of tissues with higher levels in the brain and testis. Its alternates names include Nup358 due to its role as a nucleoporin. Mechanically RanBP2 facilitates nucleocytoplasmic transport by interacting with the small GTPase Ran.

Biological function summary

RanBP2 plays a significant role in multiple processes such as mitotic spindle assembly and chromatin condensation. It functions as part of the Ran GTPase system a critical complex for nucleocytoplasmic transport. In addition RanBP2 contributes to protein sumoylation where it acts as a scaffold for conjugating enzymes. It maintains nuclear envelope integrity and supports the positioning of centrosomes during cell division.

Pathways

RanBP2 is actively involved in the nucleocytoplasmic transport pathway ensuring the proper exchange of proteins and RNA between the nucleus and cytoplasm. It is critical to the Ran cycle where it works alongside proteins like RanGTP and RanGAP1 to regulate transport activities. The involvement of RanBP2 in the SUMOylation pathway also emphasizes its role in post-translational modification impacting various cellular events and signaling.

Changes in RanBP2 expression have been linked to neurodegenerative diseases and certain types of cancer. In neurodegenerative conditions such as hereditary spastic paraplegia RanBP2 forms a complex with proteins like atlastin-1 which further influences disease progression. In cancer RanBP2 interacts with oncogenic pathways through its role in cell cycle regulation highlighting its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I (PubMed : 11792325, PubMed : 12032081, PubMed : 15378033, PubMed : 15931224, PubMed : 22194619). Involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates (PubMed : 7775481). Binds single-stranded RNA (in vitro) (PubMed : 7775481). May bind DNA (PubMed : 7775481). Component of the nuclear export pathway (PubMed : 10078529). Specific docking site for the nuclear export factor exportin-1 (PubMed : 10078529). Inhibits EIF4E-dependent mRNA export (PubMed : 22902403). Sumoylates PML at 'Lys-490' which is essential for the proper assembly of PML-NB (PubMed : 22155184). Recruits BICD2 to the nuclear envelope and cytoplasmic stacks of nuclear pore complex known as annulate lamellae during G2 phase of cell cycle (PubMed : 20386726). Probable inactive PPIase with no peptidyl-prolyl cis-trans isomerase activity (PubMed : 20676357, PubMed : 23353830).

See full target information RANBP2

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research 38:33 PubMed30678687

2019

Nucleolar and spindle associated protein 1 promotes metastasis of cervical carcinoma cells by activating Wnt/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Han Li,Weijing Zhang,Ming Yan,Jiaqi Qiu,Jueming Chen,Xiaoying Sun,Xiangfu Chen,Libing Song,Yanna Zhang

Journal of virology 90:5808-5823 PubMed27076642

2016

Roles of Capsid-Interacting Host Factors in Multimodal Inhibition of HIV-1 by PF74.

Applications

Unspecified application

Species

Unspecified reactive species

Akatsuki Saito,Damien Ferhadian,Gregory A Sowd,Erik Serrao,Jiong Shi,Upul D Halambage,Samantha Teng,Juan Soto,Mohammad Adnan Siddiqui,Alan N Engelman,Christopher Aiken,Masahiro Yamashita

PLoS pathogens 10:e1003868 PubMed24415937

2014

In vivo functions of CPSF6 for HIV-1 as revealed by HIV-1 capsid evolution in HLA-B27-positive subjects.

Applications

Species

Human

Matthew S Henning,Brittany N Dubose,Mallori J Burse,Christopher Aiken,Masahiro Yamashita

PLoS pathogens 7:e1001313 PubMed21423673

2011

HIV integration targeting: a pathway involving Transportin-3 and the nuclear pore protein RanBP2.

Applications

Species

Human

Karen E Ocwieja,Troy L Brady,Keshet Ronen,Alyssa Huegel,Shoshannah L Roth,Torsten Schaller,Leo C James,Greg J Towers,John A T Young,Sumit K Chanda,Renate König,Nirav Malani,Charles C Berry,Frederic D Bushman

FEBS letters 585:791-6 PubMed21310149

2011

Mice lacking Ran binding protein 1 are viable and show male infertility.

Applications

IHC

Species

Mouse

Masahiro Nagai,Tetsuji Moriyama,Rashid Mehmood,Keizo Tokuhiro,Masahito Ikawa,Masaru Okabe,Hiromitsu Tanaka,Yoshihiro Yoneda

PloS one 5:e15620 PubMed21179483

2010

Perturbation of host nuclear membrane component RanBP2 impairs the nuclear import of human immunodeficiency virus -1 preintegration complex (DNA).

Applications

Species

Human

Ruonan Zhang,Rajeev Mehla,Ashok Chauhan

Cancer letters 278:41-8 PubMed19171422

2009

SAGE analysis highlights the importance of p53csv, ddx5, mapkapk2 and ranbp2 to multiple myeloma tumorigenesis.

Applications

IHC-P

Species

Human

Roberta S Felix,Gisele W B Colleoni,Otavia L Caballero,Mihoko Yamamoto,Manuella S S Almeida,Valéria C C Andrade,Maria de Lourdes L F Chauffaille,Wilson A da Silva,Maria Dirlei Begnami,Fernando Augusto Soares,Andrew J Simpson,Marco Antonio Zago,André L Vettore

Gastroenterology 134:1961-71, 1971.e1-4 PubMed18439914

2008

Regulation of Wnt signaling by the nuclear pore complex.

Applications

Unspecified application

Species

Unspecified reactive species

Miki Shitashige,Reiko Satow,Kazufumi Honda,Masaya Ono,Setsuo Hirohashi,Tesshi Yamada

The Journal of biological chemistry 282:34581-93 PubMed17897946

2007

Identification of human T cell leukemia virus type 1 tax amino acid signals and cellular factors involved in secretion of the viral oncoprotein.

Applications

Dot

Species

Unspecified reactive species

Pooja Jain,Kate Mostoller,Katherine E Flaig,Jaya Ahuja,Veronique Lepoutre,Timothy Alefantis,Zafar K Khan,Brian Wigdahl

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-RanBP2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP2 antibody (AB2938)

Western blot - Anti-RanBP2 antibody (AB2938)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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