AB64276

Anti-RanBP2 antibody

Name: Anti-RanBP2 antibody. KO tested (ab64276) | Abcam
Brand: Abcam Limited
SKU: AB64276
Price: 505 USD
Availability: InStock
Rating: 5 (4 reviews)

KO Validated
What is this?

(4 Reviews)

(38 Publications)

Rabbit Polyclonal RanBP2 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 38 publications. Immunogen corresponding to Synthetic Peptide within Human RANBP2 aa 1550-1650.

View Alternative Names

NUP358, RANBP2, E3 SUMO-protein ligase RanBP2, 358 kDa nucleoporin, Nuclear pore complex protein Nup358, Nucleoporin Nup358, Ran-binding protein 2, p270, RanBP2

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-RanBP2 antibody (AB64276)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RanBP2 antibody (AB64276)

ab64276 (1 : 2000) staining RanBP2 in Hela cells pre-extracted in 0.005% digitonin and fixed in 3.7% PFA/30 mM sucrose/PBS for 15 min (room temperature). Image kindly provided by Patrizia Lavia, Univ. "La Sapienza", CNR, Italy.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RanBP2 antibody (AB64276)

Immunofluorescent staining of paraformaldehyde fixed HeLa cells by ab64276 (1 : 2000).

Unknown

Western blot - Anti-RanBP2 antibody (AB64276)

WB performed on a 6%SDS-PAGE gel. Image Kindly provided by Patrizia Lavia, University "La Sapienza", CNR, Italy.

All lanes:

Western blot - Anti-RanBP2 antibody (ab64276) at 1/2000 dilution

All lanes:

Hela whole cell lysate

Predicted band size: 358 kDa

false

Exposure time: 1min

Lab

Western blot - Anti-RanBP2 antibody (AB64276)

Lanes 1 - 4 : Merged signal (red and green). Green - ab64276 observed at 450 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

ab64276 was shown to react with RanBP2 in wild-type HeLa cells in Western blot with loss of signal observed in RANBP2 knockout cell line ab265618 (RANBP2 knockout cell lysate ab257627). Wild-type HeLa and RANBP2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab64276 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 : 1000 dilution and 1 : 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 : 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-RanBP2 antibody (ab64276) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

RANBP2 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human RANBP2 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-ranbp2-knockout-hela-cell-line-ab265618'>ab265618</a>)

Lane 3:

Caco2 cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Predicted band size: 358 kDa

Observed band size: 450 kDa

false

Unknown

Western blot - Anti-RanBP2 antibody (AB64276)

All lanes:

Western blot - Anti-RanBP2 antibody (ab64276) at 1/1000 dilution

All lanes:

HeLa total cell lysate

Predicted band size: 358 kDa

Observed band size: 358 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IP, WB

applications

Immunogen

Synthetic Peptide within Human RANBP2 aa 1550-1650. The exact immunogen used to generate this antibody is proprietary information.

P49792

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

pH: 6 - 8.5 Preservative: 0.09% Sodium azide Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RanBP2 also known as Nucleoporin 358 (Nup358) is a large protein with a molecular mass of approximately 358 kDa. It is an important component of the nuclear pore complex (NPC) and is found on the cytoplasmic side of the nuclear envelope. RanBP2 is expressed in a variety of tissues with higher levels in the brain and testis. Its alternates names include Nup358 due to its role as a nucleoporin. Mechanically RanBP2 facilitates nucleocytoplasmic transport by interacting with the small GTPase Ran.

Biological function summary

RanBP2 plays a significant role in multiple processes such as mitotic spindle assembly and chromatin condensation. It functions as part of the Ran GTPase system a critical complex for nucleocytoplasmic transport. In addition RanBP2 contributes to protein sumoylation where it acts as a scaffold for conjugating enzymes. It maintains nuclear envelope integrity and supports the positioning of centrosomes during cell division.

Pathways

RanBP2 is actively involved in the nucleocytoplasmic transport pathway ensuring the proper exchange of proteins and RNA between the nucleus and cytoplasm. It is critical to the Ran cycle where it works alongside proteins like RanGTP and RanGAP1 to regulate transport activities. The involvement of RanBP2 in the SUMOylation pathway also emphasizes its role in post-translational modification impacting various cellular events and signaling.

Changes in RanBP2 expression have been linked to neurodegenerative diseases and certain types of cancer. In neurodegenerative conditions such as hereditary spastic paraplegia RanBP2 forms a complex with proteins like atlastin-1 which further influences disease progression. In cancer RanBP2 interacts with oncogenic pathways through its role in cell cycle regulation highlighting its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I (PubMed : 11792325, PubMed : 12032081, PubMed : 15378033, PubMed : 15931224, PubMed : 22194619). Involved in transport factor (Ran-GTP, karyopherin)-mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates (PubMed : 7775481). Binds single-stranded RNA (in vitro) (PubMed : 7775481). May bind DNA (PubMed : 7775481). Component of the nuclear export pathway (PubMed : 10078529). Specific docking site for the nuclear export factor exportin-1 (PubMed : 10078529). Inhibits EIF4E-dependent mRNA export (PubMed : 22902403). Sumoylates PML at 'Lys-490' which is essential for the proper assembly of PML-NB (PubMed : 22155184). Recruits BICD2 to the nuclear envelope and cytoplasmic stacks of nuclear pore complex known as annulate lamellae during G2 phase of cell cycle (PubMed : 20386726). Probable inactive PPIase with no peptidyl-prolyl cis-trans isomerase activity (PubMed : 20676357, PubMed : 23353830).

See full target information RANBP2

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Publications (38)

Recent publications for all applications. Explore the full list and refine your search

BJOG : an international journal of obstetrics and gynaecology 132:120-131 PubMed39992005

2025

Sigma-1 Receptor-Mediated High Mobility Group A1 Silencing Alleviates Endoplasmic Reticulum Stress-Induced Ovarian Granulosa Cell Apoptosis: An In Vitro Cell Experimental Study.

Applications

Unspecified application

Species

Unspecified reactive species

Lile Jiang,Shujun Yang,Cuilian Zhang

Journal of cellular biochemistry 125:e30573 PubMed38780165

2024

Nucleoporin Nup98 is an essential factor for ipo4 dependent protein import.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Guo,Tao Tao,Ting Wu,Jingjing Hou,Wenbo Lin

The Journal of cell biology 223: PubMed38652117

2024

UBAP2L ensures homeostasis of nuclear pore complexes at the intact nuclear envelope.

Applications

Unspecified application

Species

Unspecified reactive species

Yongrong Liao,Leonid Andronov,Xiaotian Liu,Junyan Lin,Lucile Guerber,Linjie Lu,Arantxa Agote-Arán,Evanthia Pangou,Li Ran,Charlotte Kleiss,Mengdi Qu,Stephane Schmucker,Luca Cirillo,Zhirong Zhang,Daniel Riveline,Monica Gotta,Bruno P Klaholz,Izabela Sumara

Nature communications 14:3782 PubMed37355754

2023

The HIV-1 capsid core is an opportunistic nuclear import receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Guangai Xue,Hyun Jae Yu,Cindy Buffone,Szu-Wei Huang,KyeongEun Lee,Shih Lin Goh,Anna T Gres,Mehmet Hakan Guney,Stefan G Sarafianos,Jeremy Luban,Felipe Diaz-Griffero,Vineet N KewalRamani

PLoS genetics 19:e1010642 PubMed36930595

2023

Role of Nesprin-2 and RanBP2 in BICD2-associated brain developmental disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Julie Yi,Xiaoxin Zhao,Crystal R Noell,Paige Helmer,Sozanne R Solmaz,Richard B Vallee

Nature communications 13:5881 PubMed36202822

2022

RNA export through the nuclear pore complex is directional.

Applications

Unspecified application

Species

Unspecified reactive species

Asaf Ashkenazy-Titelman,Mohammad Khaled Atrash,Alon Boocholez,Noa Kinor,Yaron Shav-Tal

mBio 13:e0171422 PubMed35880880

2022

MX2 Viral Substrate Breadth and Inhibitory Activity Are Regulated by Protein Phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Gilberto Betancor,Madeleine Bangham,Jun Ki Jeon,Kanisha Shah,Steven Lynham,Jose M Jimenez-Guardeño,Michael H Malim

PLoS pathogens 18:e1010588 PubMed35709296

2022

RANBP2 and USP9x regulate nuclear import of adenovirus minor coat protein IIIa.

Applications

Unspecified application

Species

Unspecified reactive species

Ashrafali M Ismail,Amrita Saha,Ji S Lee,David F Painter,Yinghua Chen,Gurdeep Singh,Gabriela N Condezo,James Chodosh,Carmen San Martín,Jaya Rajaiya

Frontiers in cell and developmental biology 9:755847 PubMed34977012

2021

Fragile X-Related Protein 1 Regulates Nucleoporin Localization in a Cell Cycle-Dependent Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Arantxa Agote-Arán,Junyan Lin,Izabela Sumara

PLoS pathogens 17:e1009484 PubMed34543344

2021

HIV-1 requires capsid remodelling at the nuclear pore for nuclear entry and integration.

Applications

Unspecified application

Species

Unspecified reactive species

Anabel Guedán,Callum D Donaldson,Eve R Caroe,Ophélie Cosnefroy,Ian A Taylor,Kate N Bishop

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-RanBP2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RanBP2 antibody (AB64276)

Immunocytochemistry/ Immunofluorescence - Anti-RanBP2 antibody (AB64276)

Western blot - Anti-RanBP2 antibody (AB64276)

Western blot - Anti-RanBP2 antibody (AB64276)

Western blot - Anti-RanBP2 antibody (AB64276)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (38)

Product promise