Rabbit Recombinant Monoclonal RanBP3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP3 antibody [EPR5088(2)] - BSA and Azide free (AB248660), expandable thumbnail](https://content.abcam.com/products/images/ranbp3-antibody-epr50882-bsa-and-azide-free-ab248660--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img42382.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP3 antibody [EPR5088(2)] - BSA and Azide free (AB248660)")
![Western blot - Anti-RanBP3 antibody [EPR5088(2)] - BSA and Azide free (AB248660), expandable thumbnail](https://content.abcam.com/products/images/ranbp3-antibody-epr50882-bsa-and-azide-free-ab248660--western-blot-img113054.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RanBP3 antibody [EPR5088(2)] - BSA and Azide free (AB248660)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP3 antibody [EPR5088(2)] - BSA and Azide free (AB248660), expandable thumbnail](https://content.abcam.com/products/images/ranbp3-antibody-epr50882-bsa-and-azide-free-ab248660--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57708.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP3 antibody [EPR5088(2)] - BSA and Azide free (AB248660)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Target data
Function
Acts as a cofactor for XPO1/CRM1-mediated nuclear export, perhaps as export complex scaffolding protein. Bound to XPO1/CRM1, stabilizes the XPO1/CRM1-cargo interaction. In the absence of Ran-bound GTP prevents binding of XPO1/CRM1 to the nuclear pore complex. Binds to CHC1/RCC1 and increases the guanine nucleotide exchange activity of CHC1/RCC1. Recruits XPO1/CRM1 to CHC1/RCC1 in a Ran-dependent manner. Negative regulator of TGF-beta signaling through interaction with the R-SMAD proteins, SMAD2 and SMAD3, and mediating their nuclear export.
Alternative names
Ran-binding protein 3, RanBP3, RANBP3
Recommended products
Rabbit Recombinant Monoclonal RanBP3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR5088(2)
- Purification technique
- Affinity purification Protein A
- Specificity
The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248660 is the carrier-free version of Anti-RanBP3 antibody [EPR5088(2)] ab134052.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RanBP3 also known as Ran-binding protein 3 functions as a Ran-dependent nuclear export factor. It has a molecular weight of around 58 kDa. It specifically facilitates the export of proteins and RNAs from the nucleus to the cytoplasm interacting directly with the Ran GTPase. RanBP3 is ubiquitously expressed in many tissue types indicating its significant role across various cellular processes. It serves as an adapter in the formation of export complexes binding to export receptors and the cargo.
- Biological function summary
RanBP3 modulates cargo recognition and assembly of export complexes. It plays a role in the nuclear transport of proteins impacting gene regulation and cell cycle progression. As part of a larger export complex RanBP3 works together with exportin-1 and the GTP-bound form of Ran to mediate the nuclear export process efficiently. This targeted export contributes to maintaining cellular homeostasis and influences cellular responses to environmental changes.
- Pathways
RanBP3 importantly participates in the nucleocytoplasmic transport pathway. This pathway is important for the regulation of gene expression and signal transduction. RanBP3 interacts with proteins such as exportin-1 (CRM1) and CAS which coordinate the nuclear export of various proteins and RNAs. The pathway ensures the proper distribution of cellular components which is essential for cell function and survival.
- Associated diseases and disorders
RanBP3 associates with conditions like cancer and viral infections. Alterations in RanBP3 expression or function can lead to dysregulation of nuclear export impacting cell proliferation and apoptosis as observed in cancer. RanBP3's interaction with importin-beta and exportin-1 can influence the cellular response to viral infections as viruses often exploit nuclear export pathways for replication. Understanding these associations could lead to potential therapeutic interventions targeting RanBP3 activities.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP3 antibody [EPR5088(2)] - BSA and Azide free (ab248660), expandable thumbnail](https://content.abcam.com/products/images/ranbp3-antibody-epr50882-bsa-and-azide-free-ab248660--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img42382.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP3 antibody [EPR5088(2)] - BSA and Azide free (ab248660)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP3 antibody [EPR5088(2)] - BSA and Azide free (ab248660)
This data was developed using Anti-RanBP3 antibody [EPR5088(2)] ab134052, the same antibody clone in a different buffer formulation.Immunohistochemistry of Human clear cell carcinoma of kidney tissues staining RanBP3 using Anti-RanBP3 antibody [EPR5088(2)] ab134052 (1/1000). a HRP conjugated Goat Anti-Rabbit IgG H&L was used as a secondary antibody. Antigen retrieval performed using heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). image shows nuclear and cytoplasmic staining on human clear cell carcinoma of kidney.
![Western blot - Anti-RanBP3 antibody [EPR5088(2)] - BSA and Azide free (ab248660), expandable thumbnail](https://content.abcam.com/products/images/ranbp3-antibody-epr50882-bsa-and-azide-free-ab248660--western-blot-img113054.jpg "Western blot - Anti-RanBP3 antibody [EPR5088(2)] - BSA and Azide free (ab248660)")
Western blot - Anti-RanBP3 antibody [EPR5088(2)] - BSA and Azide free (ab248660)
This data was developed using Anti-RanBP3 antibody [EPR5088(2)] ab134052, the same antibody clone in a different buffer formulation.
The double bands are consistent with what has been described in PMID 16314428.
We are unsure how to define the extra bands.
All lanes: Western blot - Anti-RanBP3 antibody [EPR5088(2)] (Anti-RanBP3 antibody [EPR5088(2)] ab134052) at 1/1000 dilution
Lane 1: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 2: MEF (Mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 3: Y79 (Human retinoblastoma retinoblastoma) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/200000 dilution
Predicted band size: 60 kDa
Observed band size: 70 kDa, 90 kDa
Exposure time: 180s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP3 antibody [EPR5088(2)] - BSA and Azide free (ab248660), expandable thumbnail](https://content.abcam.com/products/images/ranbp3-antibody-epr50882-bsa-and-azide-free-ab248660--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57708.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP3 antibody [EPR5088(2)] - BSA and Azide free (ab248660)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP3 antibody [EPR5088(2)] - BSA and Azide free (ab248660)
This data was developed using Anti-RanBP3 antibody [EPR5088(2)] ab134052, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling RanBP3 with Anti-RanBP3 antibody [EPR5088(2)] ab134052 at 1/100 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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