JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB140627

Anti-RanBP9 antibody [EPR9920(B)]

4

(4 Reviews)

|

(3 Publications)

Rabbit Recombinant Monoclonal RanBP9 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

RANBPM, RANBP9, Ran-binding protein 9, RanBP9, BPM-L, BPM90, Ran-binding protein M, RanBP7, RanBPM

2 Images
Western blot - Anti-RanBP9 antibody [EPR9920(B)] (AB140627)
  • WB

Unknown

Western blot - Anti-RanBP9 antibody [EPR9920(B)] (AB140627)

All lanes:

Western blot - Anti-RanBP9 antibody [EPR9920(B)] (ab140627) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Fetal brain tissue lysate at 10 µg

Lane 3:

JAR cell lysate at 10 µg

Predicted band size: 78 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-RanBP9 antibody [EPR9920(B)] (AB140627)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RanBP9 antibody [EPR9920(B)] (AB140627)

Immunofluorescent staining of HeLa cells labelling RanBP9 with ab140627 at 1/100 dilution.

  • Carrier free

    Anti-RanBP9 antibody [EPR9920(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9920(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RanBP9 also known as Ran-binding protein 9 is a multifunctional protein with a molecular mass of approximately 85 kDa. It plays a significant role in various cellular functions by interacting with Ran a small GTPase involved in nucleocytoplasmic transport. RanBP9 is expressed in multiple tissues with higher levels observed in the brain testis and kidney indicating its diverse involvement in cellular processes. The protein has functional domains that allow it to interact with other proteins and contribute to cell signaling and structure.
Biological function summary

This protein acts as an adaptor within a variety of cellular complexes where it mediates protein-protein interactions. It influences several processes including cell migration cytoskeletal organization and neurite outgrowth. RanBP9 forms complexes with several key signaling proteins and structural components which enables it to modulate cellular dynamics and developmental pathways. It also plays a role in apoptosis and gene transcription indicating its far-reaching effects on cellular behavior.

Pathways

Several cell signaling and transportation pathways involve RanBP9. It is notably active in the Wnt and MAPK pathways where it modulates signal transduction and cellular responses to environmental cues. In the Wnt pathway RanBP9 interacts with proteins like Dishevelled and β-Catenin influencing cell fate decisions and morphogenesis. In the MAPK pathway RanBP9 associates with key kinases that regulate cell growth differentiation and stress responses.

Alterations in RanBP9 expression or function are linked to conditions such as Alzheimer's disease and cancer. In Alzheimer's RanBP9 is thought to interact with amyloid precursor protein (APP) influencing amyloid-beta production a hallmark of the disease. In cancer RanBP9 modulates pathways that affect cell proliferation and survival involving proteins such as c-Met which is associated with tumor growth and metastasis. Understanding RanBP9's role in these diseases may offer targets for therapeutic interventions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

May act as scaffolding protein, and as adapter protein to couple membrane receptors to intracellular signaling pathways (Probable). Acts as a mediator of cell spreading and actin cytoskeleton rearrangement (PubMed : 18710924). Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1 (PubMed : 29911972). May be involved in signaling of ITGB2/LFA-1 and other integrins (PubMed : 14722085). Enhances HGF-MET signaling by recruiting Sos and activating the Ras pathway (PubMed : 12147692). Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but not affect estrogen-induced transactivation (PubMed : 12361945, PubMed : 18222118). Stabilizes TP73 isoform Alpha, probably by inhibiting its ubiquitination, and increases its proapoptotic activity (PubMed : 15558019). Inhibits the kinase activity of DYRK1A and DYRK1B. Inhibits FMR1 binding to RNA.
See full target information RANBP9
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:171 PubMed40223093

2025

An in vivo "turning model" reveals new RanBP9 interactions in lung macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuko Kajimura,Shuxin Dong,Anna Tessari,Arturo Orlacchio,Alexandra Thoms,Maria Concetta Cufaro,Federica Di Marco,Foued Amari,Min Chen,Shimaa H A Soliman,Lara Rizzotto,Liwen Zhang,Damu Sunilkumar,Joseph M Amann,David P Carbone,Amer Ahmed,Giuseppe Fiermonte,Mike A Freitas,Alessia Lodi,Piero Del Boccio,Lino Tessarollo,Dario Palmieri,Vincenzo Coppola

Scientific reports 10:7138 PubMed32346083

2020

Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin.

Applications

Unspecified application

Species

Unspecified reactive species

Shimaa H A Soliman,Aaron E Stark,Miranda L Gardner,Sean W Harshman,Chelssie C Breece,Foued Amari,Arturo Orlacchio,Min Chen,Anna Tessari,Jennifer A Martin,Rosa Visone,Michael A Freitas,Krista M D La Perle,Dario Palmieri,Vincenzo Coppola

PLoS genetics 10:e1004825 PubMed25474150

2014

RAN-binding protein 9 is involved in alternative splicing and is critical for male germ cell development and male fertility.

Applications

Unspecified application

Species

Unspecified reactive species

Jianqiang Bao,Chong Tang,Jiachen Li,Ying Zhang,Bhupal P Bhetwal,Huili Zheng,Wei Yan
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB205954

Anti-RanBP9 antibody

Immunoprecipitation - Anti-RanBP9 antibody (AB205954)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB187646

Anti-Uroplakin III antibody [EPR14420]

RabMAb|Recombinant|Trial Size

Flow Cytometry (Intracellular) - Anti-Uroplakin III antibody [EPR14420] (AB187646)

  • 0
  • 0
Primary Antibodies

AB133323

Anti-14-3-3 antibody [EPR6379]

RabMAb|Recombinant

Western blot - Anti-14-3-3 antibody [EPR6379] (AB133323)

  • 0
  • 0
Primary Antibodies

AB14715

Anti-SDHA antibody [2E3GC12FB2AE2]

KO Validated

Western blot - Anti-SDHA antibody [2E3GC12FB2AE2] (AB14715)

  • 5
  • 25
Primary Antibodies

AB267474

Anti-TFF2 antibody [EPR23231-208]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TFF2 antibody [EPR23231-208] (AB267474)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com