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AB248912

Anti-RanBP9 antibody [EPR9920(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal RanBP9 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

RANBPM, RANBP9, Ran-binding protein 9, RanBP9, BPM-L, BPM90, Ran-binding protein M, RanBP7, RanBPM

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-RanBP9 antibody [EPR9920(B)] - BSA and Azide free (AB248912)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RanBP9 antibody [EPR9920(B)] - BSA and Azide free (AB248912)

This data was developed using ab140627, the same antibody clone in a different buffer formulation.Immunofluorescent staining of HeLa cells labelling RanBP9 with ab140627 at 1/100 dilution.

Western blot - Anti-RanBP9 antibody [EPR9920(B)] - BSA and Azide free (AB248912)
  • WB

Unknown

Western blot - Anti-RanBP9 antibody [EPR9920(B)] - BSA and Azide free (AB248912)

This data was developed using ab140627, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RanBP9 antibody [EPR9920(B)] (<a href='/en-us/products/primary-antibodies/ranbp9-antibody-epr9920b-ab140627'>ab140627</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Fetal brain tissue lysate at 10 µg

Lane 3:

JAR cell lysate at 10 µg

Predicted band size: 78 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9920(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab248912 is the carrier-free version of ab140627.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RanBP9 also known as Ran-binding protein 9 is a multifunctional protein with a molecular mass of approximately 85 kDa. It plays a significant role in various cellular functions by interacting with Ran a small GTPase involved in nucleocytoplasmic transport. RanBP9 is expressed in multiple tissues with higher levels observed in the brain testis and kidney indicating its diverse involvement in cellular processes. The protein has functional domains that allow it to interact with other proteins and contribute to cell signaling and structure.
Biological function summary

This protein acts as an adaptor within a variety of cellular complexes where it mediates protein-protein interactions. It influences several processes including cell migration cytoskeletal organization and neurite outgrowth. RanBP9 forms complexes with several key signaling proteins and structural components which enables it to modulate cellular dynamics and developmental pathways. It also plays a role in apoptosis and gene transcription indicating its far-reaching effects on cellular behavior.

Pathways

Several cell signaling and transportation pathways involve RanBP9. It is notably active in the Wnt and MAPK pathways where it modulates signal transduction and cellular responses to environmental cues. In the Wnt pathway RanBP9 interacts with proteins like Dishevelled and β-Catenin influencing cell fate decisions and morphogenesis. In the MAPK pathway RanBP9 associates with key kinases that regulate cell growth differentiation and stress responses.

Alterations in RanBP9 expression or function are linked to conditions such as Alzheimer's disease and cancer. In Alzheimer's RanBP9 is thought to interact with amyloid precursor protein (APP) influencing amyloid-beta production a hallmark of the disease. In cancer RanBP9 modulates pathways that affect cell proliferation and survival involving proteins such as c-Met which is associated with tumor growth and metastasis. Understanding RanBP9's role in these diseases may offer targets for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as scaffolding protein, and as adapter protein to couple membrane receptors to intracellular signaling pathways (Probable). Acts as a mediator of cell spreading and actin cytoskeleton rearrangement (PubMed : 18710924). Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1 (PubMed : 29911972). May be involved in signaling of ITGB2/LFA-1 and other integrins (PubMed : 14722085). Enhances HGF-MET signaling by recruiting Sos and activating the Ras pathway (PubMed : 12147692). Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but not affect estrogen-induced transactivation (PubMed : 12361945, PubMed : 18222118). Stabilizes TP73 isoform Alpha, probably by inhibiting its ubiquitination, and increases its proapoptotic activity (PubMed : 15558019). Inhibits the kinase activity of DYRK1A and DYRK1B. Inhibits FMR1 binding to RNA.
See full target information RANBP9
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of personalized medicine 12: PubMed35207643

2022

Detyrosinated α-Tubulin, Vimentin and PD-L1 in Circulating Tumor Cells (CTCs) Isolated from Non-Small Cell Lung Cancer (NSCLC) Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Spyridoula D Katsarou,Ippokratis Messaritakis,Anastasia Voumvouraki,Stavros Kakavogiannis,Athanasios Κotsakis,Saad Alkahtani,Christos Stournaras,Stuart S Martin,Vassilis Georgoulias,Galatea Kallergi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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