Anti-RanGAP1 antibody

• ICC/IF

Collaborator

Immunocytochemistry/ Immunofluorescence - Anti-RanGAP1 antibody (AB2081)

ab2081 (1/500) detecting RanGap1 in mitotic and interphase Hela cells (green). Dapi was used as counterstain in order to highlight the nucleus (blue) and cells were fixed both in Methanol (-20 °C/ 6 mins, followed by 3 washes in PBS) or Paraformaldehyde (3,7% PFA /10 min at room temperature, 0,1% Triton/PBS 5 min, glycine 5 min, followed by 3 washes in PBS); This antibody also gave good results in NIH-3T3 cells under both the above mentioned fixation procedures (data not shown).

Image courtesy of Marilena Ciciarello and Patrizia Lavia, University 'La Sapienza' CNR, Italy

• WB

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Western blot - Anti-RanGAP1 antibody (AB2081)

ab2081 at a concentration of 0.2μg/ml staining approximately 70kda RanGAP1 in 3T3 whole cell lysate (RIPA buffer, 35μg total protein per lane) by Western blot (ECL). Primary incubation = 1 hour. ab2081 at a concentration of 0.2µg/ml staining approximately 70kda RanGAP1 in 3T3 whole cell lysate (RIPA buffer, 35µg total protein per lane) by Western blot (ECL). Primary incubation = 1 hour.

All lanes:

Western blot - Anti-RanGAP1 antibody (ab2081)

Predicted band size: 63 kDa

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