AB233817

Anti-RANK antibody [8H8B2]

Name: Anti-RANK antibody [8H8B2] (ab233817) | Abcam
Brand: Abcam Limited
SKU: AB233817

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(1 Publication)

Mouse Monoclonal RANK antibody. Suitable for Flow Cyt, WB and reacts with Human, Recombinant fragment - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TNFRSF11A aa 1-250.

View Alternative Names

CD265, RANK, TNFRSF11A, Tumor necrosis factor receptor superfamily member 11A, Osteoclast differentiation factor receptor, Receptor activator of NF-KB, ODFR

4 Images

Flow Cytometry - Anti-RANK antibody [8H8B2] (AB233817)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-RANK antibody [8H8B2] (AB233817)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling RANK with ab233817 at 1/200 dilution (green) compared with a negative control (red).

Flow Cyt

Supplier Data

Flow Cytometry - Anti-RANK antibody [8H8B2] (AB233817)

Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cells labeling RANK with ab233817 at 1/200 dilution (green) compared with a negative control (red).

Supplier Data

Western blot - Anti-RANK antibody [8H8B2] (AB233817)

Expected recombinant MW is 46 kDa.

All lanes:

Western blot - Anti-RANK antibody [8H8B2] (ab233817) at 1/500 dilution

All lanes:

Recombinant human RANK (aa 30-212) protein

Predicted band size: 66 kDa

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Supplier Data

Western blot - Anti-RANK antibody [8H8B2] (AB233817)

All lanes:

Western blot - Anti-RANK antibody [8H8B2] (ab233817) at 1/500 dilution

Lane 1:

Untransfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

RANK (aa 30-212)-hIgG-Fc-transfected HEK-293 cell lysate

Predicted band size: 66 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8H8B2

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

Flow Cyt, WB

applications

Immunogen

Recombinant Fragment Protein within Human TNFRSF11A aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6Q6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Recombinant fragment - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis (PubMed : 9878548). Its interaction with EEIG1 promotes osteoclastogenesis via facilitating the transcription of NFATC1 and activation of PLCG2 (By similarity). Involved in the regulation of interactions between T-cells and dendritic cells (By similarity).

See full target information TNFRSF11A

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Archives of medical science : AMS 20:1631-1654 PubMed39649262

2023

Oxymatrine inhibits the development of radioresistance in NSCLC cells by reversing EMT through the DcR3/AKT/GSK-3β pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianming Tang,Yu Cao,Hong Zhang,Rui Wang

View all publications

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Product promise

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