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Rabbit Monoclonal RANK antibody. Suitable for WB and reacts with Human, Rat, Mouse samples.

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Images

Western blot - Anti-RANK antibody [EPR26196-15] (AB305233), expandable thumbnail
  • Western blot - Anti-RANK antibody [EPR26196-15] (AB305233), expandable thumbnail
  • Western blot - Anti-RANK antibody [EPR26196-15] (AB305233), expandable thumbnail
  • Western blot - Anti-RANK antibody [EPR26196-15] (AB305233), expandable thumbnail
  • Western blot - Anti-RANK antibody [EPR26196-15] (AB305233), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-PFlow Cyt (Intra)IP
Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Target data

Function

Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis (PubMed:20483727, PubMed:23478294, PubMed:9878548). Its interaction with EEIG1 promotes osteoclastogenesis via facilitating the transcription of NFATC1 and activation of PLCG2 (PubMed:23478294). Involved in the regulation of interactions between T-cells and dendritic cells (PubMed:9367155).

Alternative names

Recommended products

Rabbit Monoclonal RANK antibody. Suitable for WB and reacts with Human, Rat, Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR26196-15

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-RANK antibody [EPR26196-15] (ab305233), expandable thumbnail

    Western blot - Anti-RANK antibody [EPR26196-15] (ab305233)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-RANK antibody [EPR26196-15] (ab305233) at 1/1000 dilution

    Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) non-membrane fraction at 20 µg

    Lane 2: K-562 membrane fraction at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 80 kDa

    Exposure time: 3min

  • Western blot - Anti-RANK antibody [EPR26196-15] (ab305233), expandable thumbnail

    Western blot - Anti-RANK antibody [EPR26196-15] (ab305233)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-RANK antibody [EPR26196-15] (ab305233) at 1/1000 dilution

    Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Lane 2: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 3: Rat thymus tissue lysate at 20 µg

    Lane 4: Rat spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 80 kDa

    Exposure time: 3min

  • Western blot - Anti-RANK antibody [EPR26196-15] (ab305233), expandable thumbnail

    Western blot - Anti-RANK antibody [EPR26196-15] (ab305233)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: SW480, HCT 116 (PMID:33795653).

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-RANK antibody [EPR26196-15] (ab305233) at 1/1000 dilution

    Lane 1: HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: HCT 116 at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 80 kDa

    Exposure time: 3min

  • Western blot - Anti-RANK antibody [EPR26196-15] (ab305233), expandable thumbnail

    Western blot - Anti-RANK antibody [EPR26196-15] (ab305233)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-RANK antibody [EPR26196-15] (ab305233) at 1/1000 dilution

    Lane 1: C2C12 (mouse myoblast) whole cell lysate at 20 µg

    Lane 2: Mouse brain tissue lysate at 20 µg

    Lane 3: Mouse thymus tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 80 kDa

    Exposure time: 3min

  • Western blot - Anti-RANK antibody [EPR26196-15] (ab305233), expandable thumbnail

    Western blot - Anti-RANK antibody [EPR26196-15] (ab305233)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: MCF7 (PMID: 23696795).

    Exposure time: Lanes 1-2: 92 seconds;
    Lanes 3-4: 180 seconds.

    All lanes: Western blot - Anti-RANK antibody [EPR26196-15] (ab305233) at 1/1000 dilution

    Lanes 1 and 3: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lanes 2 and 4: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 80 kDa

    Exposure time: 92s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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