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AB325015

Anti-RANK antibody [EPR29938-554]

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Rabbit Recombinant Monoclonal RANK antibody. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD265, RANK, TNFRSF11A, Tumor necrosis factor receptor superfamily member 11A, Osteoclast differentiation factor receptor, Receptor activator of NF-KB, ODFR

3 Images
Flow Cytometry - Anti-RANK antibody [EPR29938-554] (AB325015)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-RANK antibody [EPR29938-554] (AB325015)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling RANK with ab325015 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable lymphoid cells.
Cells were co-stained with anti-CD3 conjugated to Alexa Fluor®647.

Flow Cytometry - Anti-RANK antibody [EPR29938-554] (AB325015)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-RANK antibody [EPR29938-554] (AB325015)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling RANK with ab325015 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable lymphoid cells.
Cells were co-stained with anti-CD56 conjugated to Brilliant Violet 421.

Flow Cytometry - Anti-RANK antibody [EPR29938-554] (AB325015)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-RANK antibody [EPR29938-554] (AB325015)

Flow cytometric analysis of Daudi (human Burkitt's lymphoma lymphoblast, Left) / MJ [G11] (human Peripheral blood T lymphoblast, Right) cells labelling RANK with ab325015 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Negative control : Daudi.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29938-554

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis (PubMed : 9878548). Its interaction with EEIG1 promotes osteoclastogenesis via facilitating the transcription of NFATC1 and activation of PLCG2 (By similarity). Involved in the regulation of interactions between T-cells and dendritic cells (By similarity).
See full target information TNFRSF11A
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com