AB276057

Anti-RANKL antibody [101] - BSA and Azide free

Recombinant
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Rabbit Recombinant Monoclonal RANKL antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TNFSF11 aa 50 to C-terminus.

View Alternative Names

CD254, OPGL, RANKL, TRANCE, TNFSF11, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, ODF

2 Images

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Immunoprecipitation - Anti-RANKL antibody [101] - BSA and Azide free (AB276057)

RANKL was immunoprecipitated from 0.5 mg of Raji (human Burkitt's lymphoma cell line) whole cell lysate with 0.5 μL of ab276057 and 15 μl of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using ab276057 at 1/500 dilution. Rabbit IgG (H+L) Dylight 800-labeled antibody, was used as secondary antibody at 1/5000 dilution.

Developed using the odssey technique.
Performed under reducing conditions.

All lanes:

Immunoprecipitation - Anti-RANKL antibody [101] - BSA and Azide free (ab276057)

Predicted band size: 35 kDa

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Western blot - Anti-RANKL antibody [101] - BSA and Azide free (AB276057)

Developed using the Odyssey technique.
Performed under reducing conditions.

All lanes:

Western blot - Anti-RANKL antibody [101] - BSA and Azide free (ab276057) at 1/500 dilution

All lanes:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) membrane lysate at 30 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

101

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Recombinant Fragment Protein within Human TNFSF11 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O14788

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>0.2-1 μL/mg of lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (PubMed : 22664871). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (By similarity).

See full target information TNFSF11

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RANKL antibody [101] - BSA and Azide free

Immunoprecipitation - Anti-RANKL antibody [101] - BSA and Azide free (AB276057)

Western blot - Anti-RANKL antibody [101] - BSA and Azide free (AB276057)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise