Anti-RANKL antibody [12A668] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANKL antibody [12A668] - BSA and Azide free (AB45039)

Paraffin embedded human liver tissue stained for RANKL with ab45039 at a 1/500 dilution. Peroxidase-conjugate and DAB chromogen. A 2 hour incubation at RT was used.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RANKL antibody [12A668] - BSA and Azide free (AB45039)

ICC/IF image of ab45039 stained HeLa cells. The cells were 4% formaldehyde fixed (10 minutes) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45039, 10 μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor^® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 μM.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANKL antibody [12A668] - BSA and Azide free (AB45039)

Paraffin embedded human lymph node tissue stained for RANKL with ab45039 at 5 μg/ml.

• WB

Supplier Data

Western blot - Anti-RANKL antibody [12A668] - BSA and Azide free (AB45039)

Band detected at ~35kDa and ~28kDa. (Expected MW of 35.5kDa according to NP_003692.1 and of 27.7kDa according to NP_143026.1)

All lanes:

Western blot - Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039) at 0.5 µg/mL

All lanes:

human lymph node lysate in RIPA buffer at 35 µg

Predicted band size: 35 kDa

Observed band size: 28 kDa

false

• WB

Unknown

Western blot - Anti-RANKL antibody [12A668] - BSA and Azide free (AB45039)

All lanes:

Western blot - Anti-RANKL antibody [12A668] - BSA and Azide free (ab45039) at 2 µg

All lanes:

transfected cell lysate

Predicted band size: 35 kDa

Observed band size: 35 kDa

false