Rabbit Polyclonal RANKL antibody. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human TNFSF11 aa 250 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- Nature communications 14:44172023Single-cell transcriptomics of human cholesteatoma identifies an activin A-producing osteoclastogenic fibroblast subset inducing bone destruction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKotaro Shimizu,Junichi Kikuta,Yumi Ohta,Yutaka Uchida,Yu Miyamoto,Akito Morimoto,Shinya Yari,Takashi Sato,Takefumi Kamakura,Kazuo Oshima,Ryusuke Imai,Yu-Chen Liu,Daisuke Okuzaki,Tetsuya Hara,Daisuke Motooka,Noriaki Emoto,Hidenori Inohara,Masaru IshiiPubMed 37537159
- Iranian journal of basic medical sciences 26:785-7902023Therapeutic effects of diosgenin on alveolar bone loss and apoptosis in diabetic rats with experimental periodontitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlper Kızıldağa,Aysan Lektemür Alpanb,Melih Özdedec,Tuğba Aydınd,Özlem ÖzmenePubMed 37396943
- Cureus 14:e287962022Effects of Vitamin C on the Oral-Nasal Mucosal Damage Caused by Favipiravir in Old and Young Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYücel Kurt,Özlem ÖzmenPubMed 36225521
- Life (Basel, Switzerland) 12:2022The Effect of IFT80 Deficiency in Osteocytes on Orthodontic Loading-Induced and Physiologic Bone Remodeling: In Vivo Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyeran Helen Jeon,Jessica Kang,Jiahui Madelaine Li,Douglas Kim,Gongsheng Yuan,Nicolette Almer,Min Liu,Shuying YangPubMed 36013326
- Journal of evolutionary biochemistry and physiology 58:430-4402022Evaluation of the Effects of Favipiravir Combined with Vitamin C on Alveolar Bone in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesA Lektemur Alpan,G Torumtay Cin,Ö Özmen,M F Doğan,Y ŞahinPubMed 35599639
- Frontiers in immunology 13:8399292022NLRP3 Is Involved in Neutrophil Mobilization in Experimental Periodontitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBanndith Cheat,Coralie Torrens,Asmaa Foda,Brigitte Baroukh,Jeremy Sadoine,Lotfi Slimani,Véronique Witko-Sarsat,Olivier Huck,Marjolaine Gosset,Jérôme BouchetPubMed 35281020
- Frontiers in physiology 12:6977382021AdipoRon Promotes the Osseointegration of Dental Implants in Mice With Type 2 Diabetes Mellitus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBoRui Huang,Wei Bi,Yang Sun,Ruixue Li,Xingwen Wu,Youcheng YuPubMed 36632609
- International journal of molecular sciences 22:2021Piezoelectric Microvibration Mitigates Estrogen Loss-Induced Osteoporosis and Promotes Piezo1, MicroRNA-29a, and Wnt3a Signaling in Osteoblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRe-Wen Wu,Wei-Shiung Lian,Yu-Shan Chen,Jih-Yang Ko,Shao-Yu Wang,Holger Jahr,Feng-Sheng WangPubMed 34502380
- The Angle orthodontist 91:664-6712021Osteoblast lineage cells and periodontal ligament fibroblasts regulate orthodontic tooth movement that is dependent on Nuclear Factor-kappa B (NF-kB) activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyeran Helen Jeon,Chia-Ying Yang,Min Kyung Shin,Jingyi Wang,Juhin Hiren Patel,Chun-Hsi Chung,Dana T GravesPubMed 33852725
- International journal of molecular medicine 47:2021Dihydroartemisinin attenuates osteoarthritis by inhibiting abnormal bone remodeling and angiogenesis in subchondral bone.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLong Ma et. al.PubMed 33448319
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TNFSF11 aa 250 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link O14788
Reactivity data
Select an application| IHC-P | Flow Cyt (Intra) | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/500.00000 | Notes Boil slides in 0.01M sodium citrate buffer (pH6) at 100°C for 15-20 minutes. Remove the slides from heat and allow them to stand at RT in buffer for 20 minutes. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Boil slides in 0.01M sodium citrate buffer (pH6) at 100°C for 15-20 minutes. Remove the slides from heat and allow them to stand at RT in buffer for 20 minutes. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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7 products for Alternative Product
Target data
Function
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (PubMed:22664871). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (By similarity).
Alternative names
CD254, OPGL, RANKL, TRANCE, TNFSF11, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, ODF
Recommended products
Rabbit Polyclonal RANKL antibody. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human TNFSF11 aa 250 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TNFSF11 aa 250 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link O14788
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANKL antibody (ab216484)
Immunohistochemical analysis of formalin-fixed and paraffin embedded human gastric carcinoma tissue labeling RANKL using ab216484 at 1/200, followed by secondary detection and DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANKL antibody (ab216484)
Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse spleen tissue labeling RANKL using ab216484 at 1/200, followed by secondary detection and DAB staining.
Flow Cytometry (Intracellular) - Anti-RANKL antibody (ab216484)
Intracellular Flow Cytometry analysis of Raji cellslabeling RANKL using ab216484 at 1/100 for 30 minutes (green) compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
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