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AB216484

Anti-RANKL antibody

5

(2 Reviews)

|

(26 Publications)

Rabbit Polyclonal RANKL antibody. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 26 publications. Immunogen corresponding to Synthetic Peptide within Human TNFSF11 aa 250 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CD254, OPGL, RANKL, TRANCE, TNFSF11, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, ODF

3 Images
Flow Cytometry (Intracellular) - Anti-RANKL antibody (AB216484)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RANKL antibody (AB216484)

Intracellular Flow Cytometry analysis of Raji cellslabeling RANKL using ab216484 at 1/100 for 30 minutes (green) compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANKL antibody (AB216484)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANKL antibody (AB216484)

Immunohistochemical analysis of formalin-fixed and paraffin embedded human gastric carcinoma tissue labeling RANKL using ab216484 at 1/200, followed by secondary detection and DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANKL antibody (AB216484)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANKL antibody (AB216484)

Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse spleen tissue labeling RANKL using ab216484 at 1/200, followed by secondary detection and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human TNFSF11 aa 250 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O14788

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Boil slides in 0.01M sodium citrate buffer (pH6) at 100°C for 15-20 minutes. Remove the slides from heat and allow them to stand at RT in buffer for 20 minutes.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Boil slides in 0.01M sodium citrate buffer (pH6) at 100°C for 15-20 minutes. Remove the slides from heat and allow them to stand at RT in buffer for 20 minutes.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (PubMed : 22664871). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (By similarity).
See full target information TNFSF11

Publications (26)

Recent publications for all applications. Explore the full list and refine your search

Journal for immunotherapy of cancer 13: PubMed40081943

2025

RANKL blockade inhibits cancer growth through reversing the tolerogenic profile of tumor-infiltrating (plasmacytoid) dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte Pilard,Patrick Roncarati,Marie Ancion,Margaux Luyckx,Michael Renard,Celia Reynders,Thomas Lerho,Florian Poulain,Diane Bruyere,Alizee Lebeau,Elodie Hendrick,Rebekah Crake,Raphael Peiffer,Marie-Julie Nokin,Olivier Peulen,Philippe Delvenne,Pascale Hubert,Michael Herfs

Biomedicines 13: PubMed40002839

2025

Angiotensin II Promotes Osteocyte RANKL Expression via AT1R Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayi Ren,Aseel Marahleh,Jinghan Ma,Fumitoshi Ohori,Takahiro Noguchi,Ziqiu Fan,Jin Hu,Kohei Narita,Angyi Lin,Hideki Kitaura

Journal of inflammation research 17:9725-9742 PubMed39624396

2024

Inhibition of EGFR Pathway Suppresses M1 Macrophage Polarization and Osteoclastogenesis, Mitigating Titanium Particle-Induced Bone Resorption.

Applications

Unspecified application

Species

Unspecified reactive species

Qiyu Jia,Lu Liu,Yunyuan Yu,Wuhuzi Wulamu,Lin Jia,Bo Liu,Hao Zheng,Zhenlei Peng,Xiaogang Zhang,Ruixia Zhu

Aging cell 23:e14254 PubMed39384404

2024

Cellular senescence by loss of Men1 in osteoblasts is critical for age-related osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuichiro Ukon,Takashi Kaito,Hiromasa Hirai,Takayuki Kitahara,Masayuki Bun,Joe Kodama,Daisuke Tateiwa,Shinichi Nakagawa,Masato Ikuta,Takuya Furuichi,Yuya Kanie,Takahito Fujimori,Shota Takenaka,Tadashi Yamamuro,Satoru Otsuru,Seiji Okada,Masakatsu Yamashita,Takeshi Imamura

Cellular and molecular life sciences : CMLS 81:423 PubMed39367914

2024

Eldecalcitol ameliorates diabetic osteoporosis and glucolipid metabolic disorder by promoting Treg cell differentiation through SOCE.

Applications

Unspecified application

Species

Unspecified reactive species

Yujun Jiang,Ruihan Gao,Qiaohui Ying,Xiaolin Li,Yaling Dai,Aimei Song,Hongrui Liu,Tomoka Hasegawa,Minqi Li

Redox biology 75:103255 PubMed39029270

2024

Inhibition of ferroptosis rescues M2 macrophages and alleviates arthritis by suppressing the HMGB1/TLR4/STAT3 axis in M1 macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuan Feng,Feiyang Meng,Fei Huo,Yumeng Zhu,Yifei Qin,Yu Gui,Hai Zhang,Peng Lin,Qian He,Yong Li,Jiejie Geng,Jiao Wu

Frontiers in bioengineering and biotechnology 12:1356158 PubMed38707505

2024

A submicron forest-like silicon surface promotes bone regeneration by regulating macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Guo Sun,Tianyu Shu,Shaoyang Ma,Meng Li,Zhiguo Qu,Ang Li

JOR spine 7:e1292 PubMed38222814

2024

Targeted screening of inflammatory mediators in spontaneous degenerative disc disease in dogs reveals an upregulation of the tumor necrosis superfamily.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Bitterli,David Schmid,Ladina Ettinger,Olga Krupkova,Frances C Bach,Marianna A Tryfonidou,Björn P Meij,Antonio Pozzi,Frank Steffen,Karin Wuertz-Kozak,Lucas A Smolders

Nature communications 14:4417 PubMed37537159

2023

Single-cell transcriptomics of human cholesteatoma identifies an activin A-producing osteoclastogenic fibroblast subset inducing bone destruction.

Applications

Unspecified application

Species

Unspecified reactive species

Kotaro Shimizu,Junichi Kikuta,Yumi Ohta,Yutaka Uchida,Yu Miyamoto,Akito Morimoto,Shinya Yari,Takashi Sato,Takefumi Kamakura,Kazuo Oshima,Ryusuke Imai,Yu-Chen Liu,Daisuke Okuzaki,Tetsuya Hara,Daisuke Motooka,Noriaki Emoto,Hidenori Inohara,Masaru Ishii

Iranian journal of basic medical sciences 26:785-790 PubMed37396943

2023

Therapeutic effects of diosgenin on alveolar bone loss and apoptosis in diabetic rats with experimental periodontitis.

Applications

Unspecified application

Species

Unspecified reactive species

Alper Kızıldağa,Aysan Lektemür Alpanb,Melih Özdedec,Tuğba Aydınd,Özlem Özmene
View all publications

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