AB65024

Anti-RANKL antibody

Name: Anti-RANKL antibody (ab65024) | Abcam
Brand: Abcam Limited
SKU: AB65024
Rating: 3 (2 reviews)

(2 Reviews)

(10 Publications)

Rabbit Polyclonal RANKL antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human TNFSF11.

View Alternative Names

CD254, OPGL, RANKL, TRANCE, TNFSF11, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, ODF

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-RANKL antibody (AB65024)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RANKL antibody (AB65024)

ab65024 at 1/500 - 1/1000 dilution staining RANKL in HepG2 cells by Immunofluorescence, in the absence (-) or presence (+) of the immunising peptide.

Unknown

Western blot - Anti-RANKL antibody (AB65024)

The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.

All lanes:

Western blot - Anti-RANKL antibody (ab65024) at 1/500 dilution

Lane 1:

HuvEc cell extract

Lane 2:

HuvEc cell extract with immunising peptide

Predicted band size: 35 kDa

Observed band size: 40 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human TNFSF11. The exact immunogen used to generate this antibody is proprietary information.

O14788

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (PubMed : 22664871). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (By similarity).

See full target information TNFSF11

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Aging 16:6334-6347 PubMed38575308

2024

45 activates SHP2 through inhibition of oxidative stress to regulate osteoblast and osteoclast differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Yaming Yang,Zheng Yan,Qi Xie,Yong Wang,Zhiying Liu,Min Lei

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 38:597-614 PubMed36680558

2023

Bone Deterioration in Response to Chronic High-Altitude Hypoxia Is Attenuated by a Pulsed Electromagnetic Field Via the Primary Cilium/HIF-1α Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxia Hao,Dan Wang,Zedong Yan,Yuanjun Ding,Juan Zhang,Juan Liu,Xi Shao,Xiyu Liu,Lu Wang,Erping Luo,Jing Cai,Da Jing

Bioengineered 13:13280-13292 PubMed35635031

2022

Convallatoxin suppresses osteosarcoma cell proliferation, migration, invasion, and enhances osteogenic differentiation by downregulating parathyroid hormone receptor 1 (PTHR1) expression and inactivating Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Liu,Ze Geng,Xiangyong Ding,Yan Lou,Xingquan Zhang

Evidence-based complementary and alternative medicine : eCAM 2021:7114139 PubMed34754319

2021

QiangGuYin Modulates the OPG/RANKL/RANK Pathway by Increasing Secretin Levels during Treatment of Primary Type I Osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xuefei Li,Longkang Cui,Wenhua Chen,Yuan Fang,Gaobo Shen,Zhen Li,Bingbing Zhang,Lianguo Wu

Molecular medicine reports 21:2113-2122 PubMed32323761

2020

The roles of mechanosensitive ion channels and associated downstream MAPK signaling pathways in PDLC mechanotransduction.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Shen,Yongchu Pan,Shuyu Guo,Lian Sun,Chi Zhang,Lin Wang

PloS one 9:e108920 PubMed25302610

2014

Identification of a subpopulation of marrow MSC-derived medullary adipocytes that express osteoclast-regulating molecules: marrow adipocytes express osteoclast mediators.

Applications

Species

Human

Vance Holt,Arnold I Caplan,Stephen E Haynesworth

International forum of allergy & rhinology 4:716-24 PubMed25060295

2014

Periostin and receptor activator of nuclear factor κ-B ligand expression in allergic fungal rhinosinusitis.

Applications

Unspecified application

Species

Unspecified reactive species

Adrienne M Laury,Roland Hilgarth,Asma Nusrat,Sarah K Wise

Annals of the rheumatic diseases 74:928-35 PubMed24431391

2014

Expression of FcRL4 defines a pro-inflammatory, RANKL-producing B cell subset in rheumatoid arthritis.

Applications

Unspecified application

Species

Human

L Yeo,H Lom,M Juarez,M Snow,C D Buckley,A Filer,K Raza,D Scheel-Toellner

Free radical biology & medicine 61:72-84 PubMed23500899

2013

Homocysteine alters the osteoprotegerin/RANKL system in the osteoblast to promote bone loss: pivotal role of the redox regulator forkhead O1.

Applications

Unspecified application

Species

Unspecified reactive species

Viji Vijayan,Mayuri Khandelwal,Kapil Manglani,Rajiv Ranjan Singh,Sarika Gupta,Avadhesha Surolia

Annals of the rheumatic diseases 70:2022-8 PubMed21742639

2011

Cytokine mRNA profiling identifies B cells as a major source of RANKL in rheumatoid arthritis.

Applications

IHC-Fr

Species

Human

Lorraine Yeo,Kai-Michael Toellner,Mike Salmon,Andrew Filer,Christopher D Buckley,Karim Raza,Dagmar Scheel-Toellner

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RANKL antibody

Immunocytochemistry/ Immunofluorescence - Anti-RANKL antibody (AB65024)

Western blot - Anti-RANKL antibody (AB65024)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (10)

Product promise