Rabbit Polyclonal RANKL antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human TNFSF11.
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Publications
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- Evidence-based complementary and alternative medicine : eCAM 2021:71141392021QiangGuYin Modulates the OPG/RANKL/RANK Pathway by Increasing Secretin Levels during Treatment of Primary Type I Osteoporosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuefei Li,Longkang Cui,Wenhua Chen,Yuan Fang,Gaobo Shen,Zhen Li,Bingbing Zhang,Lianguo WuPubMed 34754319
- Molecular medicine reports 21:2113-21222020The roles of mechanosensitive ion channels and associated downstream MAPK signaling pathways in PDLC mechanotransduction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun Shen et. al.PubMed 32323761
- PloS one 9:e1089202014Identification of a subpopulation of marrow MSC-derived medullary adipocytes that express osteoclast-regulating molecules: marrow adipocytes express osteoclast mediators.Applications:IFReactive species:HumanVance Holt,Arnold I Caplan,Stephen E HaynesworthPubMed 25302610
- International forum of allergy & rhinology 4:716-242014Periostin and receptor activator of nuclear factor κ-B ligand expression in allergic fungal rhinosinusitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdrienne M Laury et. al.PubMed 25060295
- Annals of the rheumatic diseases 74:928-352014Expression of FcRL4 defines a pro-inflammatory, RANKL-producing B cell subset in rheumatoid arthritis.
- Free radical biology & medicine 61:72-842013Homocysteine alters the osteoprotegerin/RANKL system in the osteoblast to promote bone loss: pivotal role of the redox regulator forkhead O1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesViji Vijayan,Mayuri Khandelwal,Kapil Manglani,Rajiv Ranjan Singh,Sarika Gupta,Avadhesha SuroliaPubMed 23500899
- Annals of the rheumatic diseases 70:2022-82011Cytokine mRNA profiling identifies B cells as a major source of RANKL in rheumatoid arthritis.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TNFSF11. The exact immunogen used to generate this antibody is proprietary information. Database link O14788
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (PubMed:22664871). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (By similarity).
Alternative names
CD254, OPGL, RANKL, TRANCE, TNFSF11, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, ODF
Recommended products
Rabbit Polyclonal RANKL antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human TNFSF11.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TNFSF11. The exact immunogen used to generate this antibody is proprietary information. Database link O14788
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Western blot - Anti-RANKL antibody (ab65024)
The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.
All lanes: Western blot - Anti-RANKL antibody (ab65024) at 1/500 dilution
Lane 1: HuvEc cell extract
Lane 2: HuvEc cell extract with immunising peptide
Predicted band size: 35 kDa
Observed band size: 40 kDa
Immunocytochemistry/ Immunofluorescence - Anti-RANKL antibody (ab65024)
ab65024 at 1/500 - 1/1000 dilution staining RANKL in HepG2 cells by Immunofluorescence, in the absence (-) or presence (+) of the immunising peptide.
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