Rabbit Polyclonal RANKL antibody. Suitable for ELISA, WB, sELISA, IHC-P, ICC/IF and reacts with Recombinant full length protein, Human, Recombinant full length protein - Human samples. Cited in 67 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TNFSF11.
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Publications
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- Journal of clinical medicine 12:2023Jaw Bone Invasion of Oral Squamous Cell Carcinoma Is Associated with Osteoclast Count and Expression of Its Regulating Proteins in Patients and Organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWillem W B de Kort,Wisse E Haakma,Robert J J van Es,Debby Gawlitta,Else Driehuis,Merel Gansevoort,Stefan M WillemsPubMed 37762975
- BMC oral health 23:6392023Comparative analysis of anchorage strength and histomorphometric changes after implantation of miniscrews in adults and adolescents: an experimental study in Beagles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Zhao,TingTing Jia,Zhiqiang WangPubMed 37670309
- Medicina (Kaunas, Lithuania) 58:2022Osteoclast and Sclerostin Expression in Osteocytes in the Femoral Head with Risedronate Therapy in Patients with Hip Fractures: A Retrospective Comparative Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHwan-Hee Lee,Eun-Yong Choi,Hyun-Sik Jun,Young-Yul KimPubMed 36363523
- Journal of pineal research 73:e128142022The role of MEK1/2 and MEK5 in melatonin-mediated actions on osteoblastogenesis, osteoclastogenesis, bone microarchitecture, biomechanics, and bone formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFahima Munmun,Omair A Mohiuddin,Van T Hoang,Matthew E Burow,Bruce A Bunnell,Veronica M Sola,Agata R Carpentieri,Paula A Witt-EnderbyPubMed 35674448
- Journal of healthcare engineering 2022:93644672022lncRNA FGD5-AS1 Regulates Bone Marrow Stem Cell Proliferation and Apoptosis by Affecting miR-296-5p/STAT3 Axis in Steroid-Induced Osteonecrosis of the Femoral Head.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYadi Wu,Lun Fang,Yong Gao,Zhiqiu Zhao,Lu Zhou,Gang ZhangPubMed 35190765
- Bioactive materials 15:409-4252022Biocompatible reduced graphene oxide stimulated BMSCs induce acceleration of bone remodeling and orthodontic tooth movement through promotion on osteoclastogenesis and angiogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDelong Jiao,Jing Wang,Wenting Yu,Ke Zhang,Ning Zhang,Lingyan Cao,Xinquan Jiang,Yuxing BaiPubMed 35386350
- Oncology letters 23:962022Capecitabine inhibits epithelial-to-mesenchymal transition and proliferation of colorectal cancer cells by mediating the RANK/RANKL pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinghai Shao,Caiping Jiang,Changhui Yu,Haijian Jia,Yanli Wang,Xinli MaoPubMed 35154427
- Progress in orthodontics 23:12022Parathyroid hormone promotes maxillary expansion and reduces relapse in the repeated activation maxillary expansion rat model by regulating Wnt/β-catenin pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengting Xu,Yuan Li,Xiaoxia Feng,Wei Zheng,Zhihe Zhao,Yu LiPubMed 34978631
- Cancer diagnosis & prognosis 2:93-1002022Monostotic Fibrous Dysplasia in the Femur Strongly Expressing RANKL With Concomitant Osteoporotic Vertebral Compression Fracture: A Case Report.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEdelyn S Azurin,Norio Yamamoto,Katsuhiro Hayashi,Akihiko Takeuchi,Shinji Miwa,Kentaro Igarashi,Takashi Higuchi,Hirotaka Yonezawa,Sei Morinaga,Yohei Asano,Shiro Saito,Hiroyuki TsuchiyaPubMed 35400000
- Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 37:381-3962021RANKL Blockade Reduces Cachexia and Bone Loss Induced by Non-Metastatic Ovarian Cancer in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFabrizio Pin,Alexander J Jones,Joshua R Huot,Ashok Narasimhan,Teresa A Zimmers,Lynda F Bonewald,Andrea BonettoPubMed 34904285
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human TNFSF11. Database link O14788
Reactivity data
Select an application| ELISA | WB | sELISA | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Predicted | Tested | Expected | Expected | Expected |
| Recombinant full length protein | Expected | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant full length protein - Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info - | Notes To detect hsRANK-L by direct ELISA (using 100μl/well antibody solution) a concentration of at least 0.5μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hsRANK-L. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes To detect hsRANK-L by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANK-L is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein, Recombinant full length protein - Human | Dilution info - | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes To detect hsRANKL by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotin Anti-sRANKL antibody ab83145 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsRANKL. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein, Recombinant full length protein - Human | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein, Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (PubMed:22664871). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (By similarity).
Alternative names
CD254, OPGL, RANKL, TRANCE, TNFSF11, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, ODF
Recommended products
Rabbit Polyclonal RANKL antibody. Suitable for ELISA, WB, sELISA, IHC-P, ICC/IF and reacts with Recombinant full length protein, Human, Recombinant full length protein - Human samples. Cited in 67 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TNFSF11.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human TNFSF11. Database link O14788
- Purification technique
- Affinity purification Immunogen
- Reconstitution
- Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Although some customers have been successful in IHC we no longer batch test in this application.
We have received both positive and negative customer feedback on mouse reactivity for this antibody. Therefore, we do not guarantee this species.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-RANKL antibody (ab9957)
RANKL contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
All lanes: Western blot - Anti-RANKL antibody (ab9957) at 1 µg/mL
All lanes: Human spleen tissue lysate - total protein (ab29699) at 10 µg
SecondaryAll lanes: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 37 kDa, 72 kDa, 76 kDa
Exposure time: 90s
Sandwich ELISA - Anti-RANKL antibody (ab9957)
To detect human sRANKL by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of Biotin Anti-sRANKL antibody ab83145 is required. This biotinylated polyclonal antibody, in conjunction with an anti-human sRANKL (ab9957) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human sRANKL.
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