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AB9957

Anti-RANKL antibody

4

(4 Reviews)

|

(81 Publications)

Rabbit Polyclonal RANKL antibody. Suitable for ELISA, WB, sELISA, IHC-P, ICC/IF and reacts with Recombinant full length protein, Human, Recombinant full length protein - Human samples. Cited in 81 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TNFSF11.

View Alternative Names

CD254, OPGL, RANKL, TRANCE, TNFSF11, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, ODF

2 Images
Sandwich ELISA - Anti-RANKL antibody (AB9957)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-RANKL antibody (AB9957)

To detect human sRANKL by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab83145 is required. This biotinylated polyclonal antibody, in conjunction with an anti-human sRANKL (ab9957) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human sRANKL.

Western blot - Anti-RANKL antibody (AB9957)
  • WB

Unknown

Western blot - Anti-RANKL antibody (AB9957)

RANKL contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

All lanes:

Western blot - Anti-RANKL antibody (ab9957) at 1 µg/mL

All lanes:

Human spleen tissue lysate - total protein (<a href='/en-us/products/unavailable/human-spleen-tissue-lysate-total-protein-ab29699'>ab29699</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution

Predicted band size: 35 kDa

Observed band size: 37 kDa,72 kDa,76 kDa

true

Exposure time: 90s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, sELISA, ICC/IF, ELISA, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TNFSF11.

O14788

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p>To detect hsRANK-L by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANK-L is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.</p>", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Recombinant full length protein": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p>To detect hsRANK-L by direct ELISA (using 100μl/well antibody solution) a concentration of at least 0.5μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hsRANK-L.</p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant full length protein - Human": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p>To detect hsRANKL by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with <a href='/en-us/products/primary-antibodies/biotin-srankl-antibody-ab83145'>ab83145</a> as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsRANKL.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

Product details

Although some customers have been successful in IHC we no longer batch test in this application.

We have received both positive and negative customer feedback on mouse reactivity for this antibody. Therefore, we do not guarantee this species.

Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Purification technique
Affinity purification Immunogen
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (PubMed : 22664871). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (By similarity).
See full target information TNFSF11

Publications (81)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:910 PubMed39695085

2024

MiR-378 exaggerates angiogenesis and bone erosion in collagen-induced arthritis mice by regulating endoplasmic reticulum stress.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengmeng Yang,Nan Hou,Wenxiang Cheng,Xuan Lu,Ming Wang,Shanshan Bai,Yuejun Lin,Yaofeng Wang,Sien Lin,Peng Zhang,Micky D Tortorella,Lu Feng,Gang Li

JCI insight 9: PubMed39641271

2024

Activation of connexin hemichannels enhances mechanosensitivity and anabolism in disused and aged bone.

Applications

Unspecified application

Species

Unspecified reactive species

Dezhi Zhao,Chao Tu,Lidan Zhang,Teja Guda,Sumin Gu,Jean X Jiang

Life (Basel, Switzerland) 14: PubMed39337872

2024

The Potential of Enamel Matrix Derivative in Countering Bisphosphonate-Induced Effects in Osteoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Minah Kim,Minji Choi,Yong-Dae Kwon,Joo-Young Ohe,Junho Jung

Scientific reports 14:19972 PubMed39198460

2024

Functional elucidation of the EIF4A3-circR-4225-miR-507-TNFSF11 regulatory axis in LUAD and its role in tumor progression.

Applications

Unspecified application

Species

Unspecified reactive species

Guoqiang Wang,Zijuan Zhang,Jiaxing Wang,Lu Kang,Guanmin Zheng,Baoguang Liu,Jiezhi Yang,Yangang Sun,Huahui Zeng,Zhenqiang Zhang

Cell death discovery 10:195 PubMed38670955

2024

Unraveling ferroptosis in osteogenic lineages: implications for dysregulated bone remodeling during periodontitis progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yiqi Tang,Sihui Su,Rongcheng Yu,Chenxi Liao,Zhili Dong,Chengyao Jia,Vicky Yau,Liping Wu,Weimin Guo,Jinxuan Zheng

Journal of translational medicine 22:340 PubMed38594779

2024

Overexpression of TNFSF11 reduces GPX4 levels and increases sensitivity to ferroptosis inducers in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zizhen Li,Wenhua Lu,Feng Yin,Peiting Zeng,Heping Li,Amin Huang

Aging 16:6334-6347 PubMed38575308

2024

45 activates SHP2 through inhibition of oxidative stress to regulate osteoblast and osteoclast differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Yaming Yang,Zheng Yan,Qi Xie,Yong Wang,Zhiying Liu,Min Lei

Journal of clinical medicine 12: PubMed37762975

2023

Jaw Bone Invasion of Oral Squamous Cell Carcinoma Is Associated with Osteoclast Count and Expression of Its Regulating Proteins in Patients and Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Willem W B de Kort,Wisse E Haakma,Robert J J van Es,Debby Gawlitta,Else Driehuis,Merel Gansevoort,Stefan M Willems

BMC oral health 23:639 PubMed37670309

2023

Comparative analysis of anchorage strength and histomorphometric changes after implantation of miniscrews in adults and adolescents: an experimental study in Beagles.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhao,TingTing Jia,Zhiqiang Wang

Cell death discovery 9:185 PubMed37344459

2023

TRPS1 regulates the opposite effect of progesterone via RANKL in endometrial carcinoma and breast carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Linlin Yang,Qiong Fan,Jing Wang,Xiaoming Yang,Jiangjing Yuan,Yuhong Li,Xiao Sun,Yudong Wang
View all publications

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