Anti-RANTES antibody [EPR26519-16]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANTES antibody [EPR26519-16] (AB307712)

Immunohistochemical analysis of paraffin-embedded human epityphlon tissue labeling RANTES with ab307712 at 1/100 dilution (5.09 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on immune cells of human epityphlon lymph node. The section was incubated with ab307712 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANTES antibody [EPR26519-16] (AB307712)

Immunohistochemical analysis of paraffin-embedded cells labeling RANTES with ab307712 at 1/500 dilution (1.018 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on (A) NK-92 cells, no staining on (B) Raji cells. The section was incubated with ab307712 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANTES antibody [EPR26519-16] (AB307712)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling RANTES with ab307712 at 1/100 dilution (5.09 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human spleen (PMID : 30986974). The section was incubated with ab307712 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANTES antibody [EPR26519-16] (AB307712)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling RANTES with ab307712 at 1/100 dilution (5.09 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on immune cells of human colon cancer. The section was incubated with ab307712 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-RANTES antibody [EPR26519-16] (AB307712)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. In Western blot, anti- H3 antibody (ab176842) loading control staining at 1/100000 dilution. Exposure time : 70 seconds.

All lanes:

Western blot - Anti-RANTES antibody [EPR26519-16] (ab307712) at 1/1000 dilution

Lane 1:

Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 2:

THP-1 treated with 100ng/ml PMA for 48 hours and 1ug/ml LPS for 8 hours, and 600ng/ml Brefeldin A for the 3 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 10 kDa

Observed band size: 10 kDa

false

Exposure time: 70s

• WB

Supplier Data

Western blot - Anti-RANTES antibody [EPR26519-16] (AB307712)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Negative control : Raji. The identity of the higher MW bands between 25kDa and 100kDa are unknown. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds.

All lanes:

Western blot - Anti-RANTES antibody [EPR26519-16] (ab307712) at 1/1000 dilution

Lane 1:

No-GFP-CD16.NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell) whole cell lysate, at 20 µg

Lane 2:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 10 kDa

Observed band size: 10 kDa

false

Exposure time: 180s