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Rabbit Recombinant Monoclonal RANTES antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANTES antibody [EPR26519-16] - BSA and Azide free (AB307713), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANTES antibody [EPR26519-16] - BSA and Azide free (AB307713), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANTES antibody [EPR26519-16] - BSA and Azide free (AB307713), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANTES antibody [EPR26519-16] - BSA and Azide free (AB307713), expandable thumbnail
  • Western blot - Anti-RANTES antibody [EPR26519-16] - BSA and Azide free (AB307713), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)IP
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) (PubMed:1380064, PubMed:15923218, PubMed:16791620, PubMed:8525373, PubMed:9516414). May also be an agonist of the G protein-coupled receptor GPR75, stimulating inositol trisphosphate production and calcium mobilization through its activation. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells (PubMed:23979485).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal RANTES antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR26519-16
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANTES antibody [EPR26519-16] - BSA and Azide free (ab307713), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANTES antibody [EPR26519-16] - BSA and Azide free (ab307713)

    This data was produced using Anti-RANTES antibody [EPR26519-16] ab307712, the same clone but in a different formulation.
    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling RANTES with Anti-RANTES antibody [EPR26519-16] ab307712 at 1/100 dilution (5.09 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Positive staining on immune cells of human colon cancer.

    The section was incubated with Anti-RANTES antibody [EPR26519-16] ab307712 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANTES antibody [EPR26519-16] - BSA and Azide free (ab307713), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANTES antibody [EPR26519-16] - BSA and Azide free (ab307713)

    This data was produced using Anti-RANTES antibody [EPR26519-16] ab307712, the same clone but in a different formulation.
    Immunohistochemical analysis of paraffin-embedded cells labeling RANTES with Anti-RANTES antibody [EPR26519-16] ab307712 at 1/500 dilution (1.018 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Positive staining on (A) NK-92 cells, no staining on (B) Raji cells.

    The section was incubated with Anti-RANTES antibody [EPR26519-16] ab307712 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANTES antibody [EPR26519-16] - BSA and Azide free (ab307713), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANTES antibody [EPR26519-16] - BSA and Azide free (ab307713)

    This data was produced using Anti-RANTES antibody [EPR26519-16] ab307712, the same clone but in a different formulation.
    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling RANTES with Anti-RANTES antibody [EPR26519-16] ab307712 at 1/100 dilution (5.09 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Positive staining on human spleen (PMID: 30986974).

    The section was incubated with Anti-RANTES antibody [EPR26519-16] ab307712 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANTES antibody [EPR26519-16] - BSA and Azide free (ab307713), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANTES antibody [EPR26519-16] - BSA and Azide free (ab307713)

    This data was produced using Anti-RANTES antibody [EPR26519-16] ab307712, the same clone but in a different formulation.
    Immunohistochemical analysis of paraffin-embedded human epityphlon tissue labeling RANTES with Anti-RANTES antibody [EPR26519-16] ab307712 at 1/100 dilution (5.09 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Positive staining on immune cells of human epityphlon lymph node.

    The section was incubated with Anti-RANTES antibody [EPR26519-16] ab307712 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-RANTES antibody [EPR26519-16] - BSA and Azide free (ab307713), expandable thumbnail

    Western blot - Anti-RANTES antibody [EPR26519-16] - BSA and Azide free (ab307713)

    This data was produced using Anti-RANTES antibody [EPR26519-16] ab307712, the same clone but in a different formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Raji.

    The identity of the higher MW bands between 25kDa and 100kDa are unknown.

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    Exposure time: 180 seconds.

    All lanes: Western blot - Anti-RANTES antibody [EPR26519-16] (Anti-RANTES antibody [EPR26519-16] ab307712) at 1/1000 dilution

    Lane 1: No-GFP-CD16.NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell) whole cell lysate, at 20 µg

    Lane 2: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 10 kDa

    Observed band size: 10 kDa

    Exposure time: 180s

  • Western blot - Anti-RANTES antibody [EPR26519-16] - BSA and Azide free (ab307713), expandable thumbnail

    Western blot - Anti-RANTES antibody [EPR26519-16] - BSA and Azide free (ab307713)

    This data was produced using Anti-RANTES antibody [EPR26519-16] ab307712, the same clone but in a different formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, anti- H3 antibody (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) loading control staining at 1/100000 dilution.

    Exposure time: 70 seconds.

    All lanes: Western blot - Anti-RANTES antibody [EPR26519-16] (Anti-RANTES antibody [EPR26519-16] ab307712) at 1/1000 dilution

    Lane 1: Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

    Lane 2: THP-1 treated with 100ng/ml PMA for 48 hours and 1ug/ml LPS for 8 hours, and 600ng/ml Brefeldin A for the 3 hours whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 10 kDa

    Observed band size: 10 kDa

    Exposure time: 70s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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