AB75871

Anti-RAP1A + RAP1B antibody [EP879Y]

Name: Anti-RAP1A + RAP1B antibody [EP879Y] (ab75871) | Abcam
Brand: Abcam Limited
SKU: AB75871
Price: 335 USD
Availability: InStock
Rating: 1 (3 reviews)

RabMAb
Recombinant
What is this?

(3 Reviews)

(4 Publications)

Rabbit Recombinant Monoclonal RAP1A antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 4 publications.

View Alternative Names

KREV1, RAP1A, Ras-related protein Rap-1A, C21KG, G-22K, GTP-binding protein smg p21A, Ras-related protein Krev-1

2 Images

Unknown

Western blot - Anti-RAP1A + RAP1B antibody [EP879Y] (AB75871)

Secondary antibody - goat anti-rabbit HRP (ab6721)

All lanes:

Western blot - Anti-RAP1A + RAP1B antibody [EP879Y] (ab75871) at 1/500 dilution

All lanes:

human brain lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

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Supplier Data

Western blot - Anti-RAP1A + RAP1B antibody [EP879Y] (AB75871)

Secondary antibody - goat anti-rabbit HRP (ab6721)

All lanes:

Western blot - Anti-RAP1A + RAP1B antibody [EP879Y] (ab75871) at 1/1000 dilution

All lanes:

Mouse brain

Predicted band size: 21 kDa

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Carrier free

Anti-RAP1A + RAP1B antibody [EP879Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP879Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together with ITGB1BP1, regulates KRIT1 localization to microtubules and membranes (PubMed : 17916086). Plays a role in nerve growth factor (NGF)-induced neurite outgrowth. Plays a role in the regulation of embryonic blood vessel formation. Involved in the establishment of basal endothelial barrier function. Facilitates the progressive accumulation of CDH1 at mature desmosome junctions via cAMP-dependent signaling and its interaction with PKP3 (PubMed : 25208567). May be involved in the regulation of the vascular endothelial growth factor receptor KDR expression at endothelial cell-cell junctions.

See full target information RAP1A

Additional targets

RAP1B

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35563009

2022

Enhanced cGMP Interactor Rap Guanine Exchange Factor 4 (EPAC2) Expression and Activity in Degenerating Photoreceptors: A Neuroprotective Response?

Applications

Unspecified application

Species

Unspecified reactive species

Michel Rasmussen,Jiaming Zhou,Frank Schwede,Per Ekström

Structure (London, England : 1993) 29:320-329.e4 PubMed33275877

2020

Phosphorylation of RIAM by src promotes integrin activation by unmasking the PH domain of RIAM.

Applications

Unspecified application

Species

Unspecified reactive species

Eun-Ah Cho,Pingfeng Zhang,Vikas Kumar,Mikhail Kavalchuk,Hao Zhang,Qingqiu Huang,James S Duncan,Jinhua Wu

Brain research bulletin 142:163-175 PubMed30031816

2018

GABAB receptor mediate hippocampal neuroinflammation in adolescent male and female mice after cold expose.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Xu,Shuai Lian,Shi-Ze Li,Jing-Ru Guo,Jian-Fa Wang,Di Wang,Li-Ping Zhang,Huan-Min Yang

Molecular biology of the cell 25:3749-64 PubMed25208567

2014

Plakophilin 3 mediates Rap1-dependent desmosome assembly and adherens junction maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Viktor Todorovic,Jennifer L Koetsier,Lisa M Godsel,Kathleen J Green

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RAP1A + RAP1B antibody [EP879Y]

Western blot - Anti-RAP1A + RAP1B antibody [EP879Y] (AB75871)

Western blot - Anti-RAP1A + RAP1B antibody [EP879Y] (AB75871)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Additional targets

Publications (4)

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