Rabbit Recombinant Monoclonal RAP1A antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.
Images
![Western blot - Anti-RAP1A + RAP1B antibody [EP879Y] - BSA and Azide free (AB247412), expandable thumbnail](https://content.abcam.com/products/images/rap1a-rap1b-antibody-ep879y-bsa-and-azide-free-ab247412--western-blot-img136552.gif "Western blot - Anti-RAP1A + RAP1B antibody [EP879Y] - BSA and Azide free (AB247412)")
![Western blot - Anti-RAP1A + RAP1B antibody [EP879Y] - BSA and Azide free (AB247412), expandable thumbnail](https://content.abcam.com/products/images/rap1a-rap1b-antibody-ep879y-bsa-and-azide-free-ab247412--western-blot-img141973.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RAP1A + RAP1B antibody [EP879Y] - BSA and Azide free (AB247412)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Flow Cyt | WB | |
|---|---|---|---|
| Human | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Tested |
| Rat | Not recommended | Not recommended | Predicted |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Target data
Function
Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together with ITGB1BP1, regulates KRIT1 localization to microtubules and membranes (PubMed:17916086). Plays a role in nerve growth factor (NGF)-induced neurite outgrowth. Plays a role in the regulation of embryonic blood vessel formation. Involved in the establishment of basal endothelial barrier function. Facilitates the progressive accumulation of CDH1 at mature desmosome junctions via cAMP-dependent signaling and its interaction with PKP3 (PubMed:25208567). May be involved in the regulation of the vascular endothelial growth factor receptor KDR expression at endothelial cell-cell junctions.
Additional Targets
RAP1B
Alternative names
KREV1, RAP1A, Ras-related protein Rap-1A, C21KG, G-22K, GTP-binding protein smg p21A, Ras-related protein Krev-1
Recommended products
Rabbit Recombinant Monoclonal RAP1A antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP879Y
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab247412 is the carrier-free version of Anti-RAP1A + RAP1B antibody [EP879Y] ab75871.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-RAP1A + RAP1B antibody [EP879Y] - BSA and Azide free (ab247412)
This data was developed using Anti-RAP1A + RAP1B antibody [EP879Y] ab75871, the same antibody clone in a different buffer formulation.
Secondary antibody - goat anti-rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) ab6721).
All lanes: Western blot - Anti-RAP1A + RAP1B antibody [EP879Y] (Anti-RAP1A + RAP1B antibody [EP879Y] ab75871) at 1/500 dilution
All lanes: human brain lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
![Western blot - Anti-RAP1A + RAP1B antibody [EP879Y] - BSA and Azide free (ab247412), expandable thumbnail](https://content.abcam.com/products/images/rap1a-rap1b-antibody-ep879y-bsa-and-azide-free-ab247412--western-blot-img141973.jpg "Western blot - Anti-RAP1A + RAP1B antibody [EP879Y] - BSA and Azide free (ab247412)")
Western blot - Anti-RAP1A + RAP1B antibody [EP879Y] - BSA and Azide free (ab247412)
This data was developed using Anti-RAP1A + RAP1B antibody [EP879Y] ab75871, the same antibody clone in a different buffer formulation.
Secondary antibody - goat anti-rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) ab6721).
All lanes: Western blot - Anti-RAP1A + RAP1B antibody [EP879Y] (Anti-RAP1A + RAP1B antibody [EP879Y] ab75871) at 1/1000 dilution
All lanes: Mouse brain
Predicted band size: 21 kDa
Downloads
Product protocols
- Visit the general protocols
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